The effect of green tea extract as a natural and ecofriendly antibacterial additive on the healing effect of chitosan/ polyethylene oxide wound dressing nanofiber was investigated. Three different polymeric nanofiber composite including chitosan, chitosan/polyethylene oxide and chitosan/polyethylene oxide/green tea extract, was produced by electrospinning method and was used as wound dressing. Affecting parameters on electrospinning method such as voltage, feed rate, nozzlecollector distance and content of green tea in nanofiber structure were studied and optimized. The morphology of electrospun nanofibers was studied with use of scanning electron microscope. The average diameters of prepared nanofibers was determined by Image J software. The optimum conditions for preparation of electrospun nanofibers were: Voltage; 20 kV, feed rate; 0.5 ml/h, nozzle-collector distance; 10 cm, and chitosan/polyethylene oxide weight ratio; 0.9. Stability, water absorption and degradation of prepared nanofibers were investigated by placing the prepared nanofibers for 24 hours in a solution of acetate buffer with a pH of 5.5 and a temperature of 37 o C. Moreover, the antibacterial activity of composite scaffolds were tested by Agar plate method by two type bacteria including Escherichia coli and Staphylococcus aureus. Furthermore, the healing ability of the prepared nanofibers was studied on the rat's wound. Chitosan/polyethylene oxide/ green tea showed the best healing effects in comparison with the other prepared wound dressings. These results confirmed that green tea extract helps to keep wound surface moist, reduces inflammation and increases the speed of recovery and healing.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.