In vivo and in situ real-time fluorescence imaging of peripheral nerves in the NIR-II window

Feng, Zhujun; Yang, Yimeng; Zhang, Jian; Wang, Kan; Li, Yunxia; Xu, Heng; Wang, Zhen; Biskup, Ewelina; Dong, Shixian; Yang, Xing; Hao, Yuefeng; Yan, Wei

doi:10.1007/s12274-019-2552-z

Cited by 21 publications

(16 citation statements)

References 42 publications

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“…The second near-infrared window (NIR-II, 1,000-1,700 nm) has made its debut by virtue of minimal scattering, high signal-to-noise ratio (SNR) and low self-fluorescence background compared with visible light and the first near-infrared window (NIR-I, 700-900 nm) (Welsher et al, 2011;Li et al, 2014;Hong et al, 2015;Chen et al, 2016;Wan et al, 2018). Lead sulfide quantum dots (PbS QDs) with emission in NIR-II window have been used to label Schwann cells when bound with motor neuron-specific protein Agrin to direct peripheral nerve imaging in vivo (Feng et al, 2019). Furthermore, stem cells were tracked by PbS QDs to observe the fate of injected cells during rotator cuff repair (Yang et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Nerve Suture Combined With ADSCs Injection Under Real-Time and Dynamic NIR-II Fluorescence Imaging in Peripheral Nerve Regeneration in vivo

et al. 2021

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Peripheral nerve injury gives rise to devastating conditions including neural dysfunction, unbearable pain and even paralysis. The therapeutic effect of current treatment for peripheral nerve injury is unsatisfactory, resulting in slow nerve regeneration and incomplete recovery of neural function. In this study, nerve suture combined with ADSCs injection was adopted in rat model of sciatic nerve injury. Under real-time visualization of the injected cells with the guidance of NIR-II fluorescence imaging in vivo, a spatio-temporal map displaying cell migration from the proximal injection site (0 day post-injection) of the nerve to the sutured site (7 days post-injection), and then to the distal section (14 days post-injection) was demonstrated. Furthermore, the results of electromyography and mechanical pain threshold indicated nerve regeneration and functional recovery after the combined therapy. Therefore, in the current study, the observed ADSCs migration in vivo, electrophysiological examination results and pathological changes all provided robust evidence for the efficacy of the applied treatment. Our approach of nerve suture combined with ADSCs injection in treating peripheral nerve injury under real-time NIR-II imaging monitoring in vivo added novel insights into the treatment for peripheral nerve injury, thus further enhancing in-depth understanding of peripheral nerve regeneration and the mechanism behind.

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Section: Introductionmentioning

confidence: 99%

Nerve Suture Combined With ADSCs Injection Under Real-Time and Dynamic NIR-II Fluorescence Imaging in Peripheral Nerve Regeneration in vivo

et al. 2021

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“…Moreover, the NIR II fluorescence imaging of gastrointestinal tract in mice by PbS/ CdS QDs was also realized at a rather low dosage. 27 Hao et al 28 exhibited a novel in vivo and in situ real-time imaging of peripheral nerves system based on NIR II emitting PbS QDs functionalized with motor neuron-derived protein (Agrin). Due to the potential binding sites on Agrin, the QDs could directly recognize peripheral nerves for targeted fluorescence imaging in a nude mouse model.…”

Section: Fluorescence Imagingmentioning

confidence: 99%

Recent advances of near infrared inorganic fluorescent probes for biomedical applications

et al. 2020

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“…AAVs N/A Local Biomolecules [23] BDA N/A Local Carbocyanine dye [23] 42] Bacterial toxins Alexa Fluor/gold NPs Local Antibody [44] Agrin antibody QDs Systemic…”

Section: Sfv Gfp Localmentioning

confidence: 99%

“…[ 43 ] Given this, Hao's group labeled the nerve‐specific antibody agrin with NIR‐II‐emitting PbS quantum dots (QDs) and visualized the PNs after systemic administration of the nanoprobe (Figure 5F‐G). [ 44 ] Zhang's group synthesized NIR‐II‐emitting PbS QDs and conjugated the QDs to the NP41 peptide via a cross‐linker containing several N ‐hydroxysuccinimide (NHS) ester maleimide groups ( Figure ). They showed that after intravenous injection of the probes in mice, the brachial plexus and sciatic nerves were noninvasively highlighted at the NIR‐II window.…”

Section: Optical Nerve Imaging Using Fluorescent Probesmentioning

confidence: 99%

Recent Advances in Intraoperative Nerve Bioimaging: Fluorescence‐Guided Surgery for Nerve Preservation

2020

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Accidental transection or injury of nerves during surgeries leads to severe patient morbidity and lowers the life quality of patients. The current imaging modality for nerves, such as magnetic resonance imaging (MRI), computerized tomography (CT), and high-resolution ultrasound, cannot provide real-time guidance for surgeons during surgical operations. Previously, intraoperative nerve recognition mainly depended on the surgeon's experience. But now, optical imaging techniques that exploit fluorescent light enable the direct intraoperative visualization of the areas of interest on a surgical site, which has the potential to improve real-time nerve identification during surgeries. Herein, a brief introduction is provided to the structure of peripheral nerve (PN) fiber and fluorescent probes for nerve labeling that are based on these features are summarized. Then, the recent progress in fluorescence-guided surgeries for nerve preservation is systematically summarized. Finally, the challenges and future opportunities for in vivo real-time nerve identification are discussed.

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In vivo and in situ real-time fluorescence imaging of peripheral nerves in the NIR-II window

Cited by 21 publications

References 42 publications

Nerve Suture Combined With ADSCs Injection Under Real-Time and Dynamic NIR-II Fluorescence Imaging in Peripheral Nerve Regeneration in vivo

Nerve Suture Combined With ADSCs Injection Under Real-Time and Dynamic NIR-II Fluorescence Imaging in Peripheral Nerve Regeneration in vivo

Recent advances of near infrared inorganic fluorescent probes for biomedical applications

Recent Advances in Intraoperative Nerve Bioimaging: Fluorescence‐Guided Surgery for Nerve Preservation

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