In vivo absorptomics: Identification of bovine milk-derived peptides in human plasma after milk intake

Caira, Simonetta; Pinto, Gabriella; Picariello, Gianluca; Vitaglione, Paola; Pascale, Sabrina De; Scaloni, Andrea; Addeo, Francesco

doi:10.1016/j.foodchem.2022.132663

Cited by 22 publications

(11 citation statements)

References 38 publications

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“…β‐Casein and its hydrolysis products are closely associated with immune system diseases, digestive system diseases, blood system diseases, and neurological diseases, among others. β‐Casein hydrolyzed by enzymes from some sources releases numerous bioactive peptides that possess unique physiological functions (Asledottir et al., 2017; Caira et al., 2022). β‐Casein bioactive peptides from bovine milk sources confirmed by research have the effects of regulating sleep, scavenging free radicals to achieve antioxidant, regulating blood pressure of organism angiotensin‐converting enzyme (ACE), and regulating immune function and so on.…”

Section: Bovine Milk Casein and β‐Caseinmentioning

confidence: 99%

Bovine milk β‐casein: Structure, properties, isolation, and targeted application of isolated products

Chen,

Fan,

Wang

et al. 2024

Comp Rev Food Sci Food Safe

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Abstractβ‐Casein, an important protein found in bovine milk, has significant potential for application in the food, pharmaceutical, and other related industries. This review first introduces the composition, structure, and functional properties of β‐casein. It then reviews the techniques for isolating β‐casein. Chemical and enzymatic isolation methods result in inactivity of β‐casein and other components in the milk, and it is difficult to control the production conditions, limiting the utilization range of products. Physical technology not only achieves high product purity and activity but also effectively preserves the biological activity of the components. The isolated β‐casein needs to be utilized effectively and efficiently for various purity products in order to achieve optimal targeted application. Bovine β‐casein, which has a purity higher than or close to that of breast β‐casein, can be used in infant formulas. This is achieved by modifying its structure through dephosphorylation, resulting in a formula that closely mimics the composition of breast milk. Bovine β‐casein, which is lower in purity than breast β‐casein, can be maximized for the preparation of functional peptides and for use as natural carriers. The remaining byproducts can be utilized as food ingredients, emulsifiers, and carriers for encapsulating and delivering active substances. Thus, realizing the intensive processing and utilization of bovine β‐casein isolation. This review can promote the industrial production process of β‐casein, which is beneficial for the sustainable development of β‐casein as a food and material. It also provides valuable insights for the development of other active substances in milk.

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Section: Bovine Milk Casein and β‐Caseinmentioning

confidence: 99%

Bovine milk β‐casein: Structure, properties, isolation, and targeted application of isolated products

Chen,

Fan,

Wang

et al. 2024

Comp Rev Food Sci Food Safe

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“…There are both conformational and linear epitopes, widely spread all along the protein molecules [ 133 , 134 ]. Bovine milk-derived peptides encrypting possible bioactive and/or immunogenic molecules originating from caseins, β-lactoglobulin, and minor milk proteins have recently been detected in human plasma after intake of pasteurized cow’s milk [ 135 ]. Cow’s milk protein allergy is a common condition encountered in young children [ 136 , 137 ], but may rarely also occur in adults [ 138 ].…”

Section: Potential Milk-related Factors Promoting Dlbclmentioning

confidence: 99%

Potential Pathogenic Impact of Cow’s Milk Consumption and Bovine Milk-Derived Exosomal MicroRNAs in Diffuse Large B-Cell Lymphoma

Melnik

Stadler

Weiskirchen

et al. 2023

IJMS

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Epidemiological evidence supports an association between cow’s milk consumption and the risk of diffuse large B-cell lymphoma (DLBCL), the most common non-Hodgkin lymphoma worldwide. This narrative review intends to elucidate the potential impact of milk-related agents, predominantly milk-derived exosomes (MDEs) and their microRNAs (miRs) in lymphomagenesis. Upregulation of PI3K-AKT-mTORC1 signaling is a common feature of DLBCL. Increased expression of B cell lymphoma 6 (BCL6) and suppression of B lymphocyte-induced maturation protein 1 (BLIMP1)/PR domain-containing protein 1 (PRDM1) are crucial pathological deviations in DLBCL. Translational evidence indicates that during the breastfeeding period, human MDE miRs support B cell proliferation via epigenetic upregulation of BCL6 (via miR-148a-3p-mediated suppression of DNA methyltransferase 1 (DNMT1) and miR-155-5p/miR-29b-5p-mediated suppression of activation-induced cytidine deaminase (AICDA) and suppression of BLIMP1 (via MDE let-7-5p/miR-125b-5p-targeting of PRDM1). After weaning with the physiological termination of MDE miR signaling, the infant’s BCL6 expression and B cell proliferation declines, whereas BLIMP1-mediated B cell maturation for adequate own antibody production rises. Because human and bovine MDE miRs share identical nucleotide sequences, the consumption of pasteurized cow’s milk in adults with the continued transfer of bioactive bovine MDE miRs may de-differentiate B cells back to the neonatal “proliferation-dominated” B cell phenotype maintaining an increased BLC6/BLIMP1 ratio. Persistent milk-induced epigenetic dysregulation of BCL6 and BLIMP1 expression may thus represent a novel driving mechanism in B cell lymphomagenesis. Bovine MDEs and their miR cargo have to be considered potential pathogens that should be removed from the human food chain.

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“…Many of these endogenous peptides are biologically active, with 271 homologous milk peptides sharing 80% sequence similarity with known bioactive peptides including ACE inhibition and antibacterial and antioxidant properties. After consuming cow milk, human plasma levels of peptides originating from bovine milk were investigated by Caira and colleagues . In this study, plasma was collected from three volunteers, separated by SEC and SPE, and analyzed using a combination of mass spectrometry (LC-MS/MS) and bioinformatics.…”

Section: Investigation Of the Effects Of Peptidomics-based Bioactivit...mentioning

confidence: 99%

“…After consuming cow milk, human plasma levels of peptides originating from bovine milk were investigated by Caira and colleagues. 126 In this study, plasma was collected from three volunteers, separated by SEC and SPE, and analyzed using a combination of mass spectrometry (LC-MS/ MS) and bioinformatics. 758, 273, and 212 milk-derived peptides from 15, 15, and 18 bovine milk proteins, respectively, were identified in the plasma of participants 1, 2, and 3, respectively.…”

Section: Enrichment Of Endogenous Peptides In Milkmentioning

confidence: 99%

Proteomics and Peptidomics As a Tool to Compare the Proteins and Endogenous Peptides in Human, Cow, and Donkey Milk

Ning,

Yang,

Liu

et al. 2023

J. Agric. Food Chem.

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Cow’s milk is the most widely used ingredient in infant formulas. However, its specific protein composition can cause allergic reactions. Finding alternatives to replace cow’s milk and fill the nutritional gap with human milk is essential for the health of infants. Proteomic and peptidomic techniques have supported the elucidation of milk’s nutritional ingredients. Recently, omics approaches have attracted increasing interest in the investigation of milk because of their high throughput, precision, sensitivity, and reproducibility. This review offers a significant overview of recent developments in proteomics and peptidomics used to study the differences in human, cow, and donkey milk. All three types of milks were identified to have critical biological functions in human health, particularly in infants. Donkey milk proteins were closer in composition to human milk, were less likely to cause allergic reactions, and may be developed as novel raw materials for formula milk powders.

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In vivo absorptomics: Identification of bovine milk-derived peptides in human plasma after milk intake

Cited by 22 publications

References 38 publications

Bovine milk β‐casein: Structure, properties, isolation, and targeted application of isolated products

Bovine milk β‐casein: Structure, properties, isolation, and targeted application of isolated products

Potential Pathogenic Impact of Cow’s Milk Consumption and Bovine Milk-Derived Exosomal MicroRNAs in Diffuse Large B-Cell Lymphoma

Proteomics and Peptidomics As a Tool to Compare the Proteins and Endogenous Peptides in Human, Cow, and Donkey Milk

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