In Vitro α-Glucosidase Inhibitory Activity of Ethanol Extract of Buas-buas (Premna serratifolia Linn)

Hadiarti, Dini

doi:10.22146/tradmedj.27917

Cited by 2 publications

(2 citation statements)

References 5 publications

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“…The flavonoid group that plays a role in inhibiting the α-glucosidase enzyme is 3', 4'-hydroxy in ring B which plays a role in interaction with the active site of the enzyme. While the 3-OH on the carbon ring functions to maintain proper binding to the flavonoid molecule 24 .…”

Section: Antidiabeticmentioning

confidence: 99%

Phytochemistry and Pharmacological Potentials of Premna serratifolia L.: Traditional Medicinal Plant Used by Local People in Kalimantan

Khairunnisa

Febriyanti²,

Muhaimin³

2022

ijbp

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Millions of people worldwide have been using herbal medicines for thousands of years, due to their great interest in traditional medicines. One of the plants in Indonesia which has medicinal properties but is not widely known by the public and used is Bebuas plant. Bebuas (Premna serratifolia Linn) has been observed growing in West Kalimantan. The people of West Kalimantan used bebuas leaves to reduce cholesterol levels, the young leaves used as a traditional medicine to expel wind in the body quickly, treat intestinal worms, and increase breast milk for nursing mothers, and the decoction of fresh leaves is used for colds and fever medicine. Several studies report bebuas (Premna serratifolia L.) had many pharmacological activities. Bebuas contains flavonoids, saponins, tannins and triterpenoids/steroids that have a role in pharmacological activity. The present review aims to summarize the current state of scientific evidence on the pharmacological potentials of P. serratifolia. The subject of literature published from 2012 to 2022 were obtained frominternational research databases including PubMed, Google Scholar, EBSCO, SCOPUS, and Cochrane, using the keywords Premna serratifolia L., traditional medicine, medicinal plants, and pharmacology. The pharmacological activities which are discussed further in the review including antioxidant, anti-arthritic, anticholesterol, anti-inflammatory, antimicrobial, antihelmintic, antidiabetic, and anticancer/cytotoxic. The study suggests that Premna serratifolia L. is a potent source for further development of phytomedicine.

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Section: Antidiabeticmentioning

confidence: 99%

Phytochemistry and Pharmacological Potentials of Premna serratifolia L.: Traditional Medicinal Plant Used by Local People in Kalimantan

Khairunnisa

Febriyanti²,

Muhaimin³

2022

ijbp

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“…Traditionally, P. serratifolia leaves have been used as a tonic, treating headache, stomachache, diabetes, hypertension, difficulty in breathing, increasing breast milk production, and malaria (Dianita and Jantan, 2017;Hasanah et al, 2015). The scientific report showed that P. serratifolia leaves have been confirmed as hepatoprotection (Vadivu et al, 2009), antifungal (Wahyuni et al, 2014), antilarval (Lestari and Yanti, 2014), antioxidant (Isnindar et al, 2016), and α-glucosidase inhibitor (Hadiarti, 2017;Hadiarti et al, 2021;Timotius et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Profiling of α-glucosidase inhibitors from ethyl acetate fraction of Buas-buas (Premna serratifolia) leaves using UHPLC-Q-Orbitrap HRMS and protein-ligand interaction with molecular docking

Hadiarti¹,

Haryadi²,

Matsjeh³

et al. 2022

J App Pharm Sc

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The Buas-buas (Premna serratifolia) leaves' extract from maceration, percolation, and decontamination have proven to be α-glucosidase inhibitors. Since the efforts to isolate phytochemical constituents from P. serratifolia leaves as α-glucosidase inhibitors have not been carried out, a study is needed to determine the responsible compound for this activity which has been proven in vitro and in silico. Isolation of active compounds from ethyl acetate fraction has the best inhibition utilizing column chromatography and semipreparative high-performance liquid chromatography (HPLC). Identifying compounds from the isolate was investigated by ultra-high-performance liquid chromatography-Q exactive hybrid quadrupole-orbitrap high-resolution mass spectrometry (UHPLC-Q-Orbitrap HRMS). Interaction of α-glucosidase and active compounds was studied by molecular docking utilizing N-terminal maltase-glucoamylase [protein data bank (PDB) code: 2QMJ], C-terminal maltase-glucoamylase (PDB code: 3TOP), and isomaltase (PDB code: 3A4A). Analyzed by UHPLC-Q-Orbitrap HRMS, nine flavonoids were detected, which are centaureidin, chrysin, pectolinaringenin, glycitein, kaempferide, syringetin, tricin, casticin, and 3,5,4ʹ-trimethoxy-6,7-methylenedioxyflavone (estimated to be a new compound). Casticin-2QMJ, tricin-3TOP, and centaureidin-3A4A complexes have the lower binding energies of −5.29, −6.77, and −8.02 kcal/mol and inhibitory constant (Ki) of 131.54, 10.89, and 0.34 µM, sequentially.

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In Vitro α-Glucosidase Inhibitory Activity of Ethanol Extract of Buas-buas (Premna serratifolia Linn)

Abstract: 0,125, 0,5, 1, 1,5, 2, dan 2,5 2% (b/v) were obtained the absorptions of 37,95, 74,77, 86,15, 91,03 and 91,69 %, respectively 0,125, 0,5, 1, 1,5, 2, dan 2,5% (b/v) diperoleh absorbsi secara berturut-turut 37,95, 74,77, 86,15, 91,03, dan

Cited by 2 publications

References 5 publications

Phytochemistry and Pharmacological Potentials of Premna serratifolia L.: Traditional Medicinal Plant Used by Local People in Kalimantan

Phytochemistry and Pharmacological Potentials of Premna serratifolia L.: Traditional Medicinal Plant Used by Local People in Kalimantan

Profiling of α-glucosidase inhibitors from ethyl acetate fraction of Buas-buas (Premna serratifolia) leaves using UHPLC-Q-Orbitrap HRMS and protein-ligand interaction with molecular docking

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