In vitro viability of human periodontal ligament cells in green tea extract

Abstract

Context:Delayed replantation of avulsed teeth may be successful if the majority of periodontal ligament cells (PDL) survive. A proper transport medium is required when immediate replantation is not possible. Green tea extract (GTE) may be effective in preserving the cells because of its special properties.Aims:This study was done to evaluate the potential of GTE in periodontal ligament cells preservation.Materials and Methods:Fifty-four extracted human teeth with closed apices were randomly divided into three groups each with 18 teeth as follow: GTE, water (negative control), and Hank's balanced salt solution (HBSS) (positive control). The specimens were immersed in the media for 1, 3, and 15 hours at 4°C (n = 6) and treated with collagenase 1A for 45 minutes. Cell viability was determined using the trypan blue exclusion technique.Statistical Analysis:Data were analyzed by one-way analysis of variance (ANOVA), post hoc Tukey and paired t-test at significance level of P < 0.05.Results:Means (standard deviation, SD) of viable cells in HBSS, water, and GTE were estimated 348.33 ± 88.49, 101 ± 14.18, and 310.56 ± 56.97 at 1 hours; 273.4 ± 44.80, 64.16 ± 16.44, and 310.2 ± 11.21 at 3 hours; and 373.72 ± 67.81, 14.41 ± 2.88 and 315.24 ± 34.48 at 15 hours; respectively. No significant differences were found between HBSS and GTE at all the time intervals. Both these solutions could preserve the cells more than water significantly.Conclusion:GTE and HBSS were equally effective in preserving the cells and were significantly superior to water.

Comparative in vitro study of the effectiveness of Green tea extract and common storage media on periodontal ligament fibroblast viability

“…Furthermore, Ghasempour et al . have shown that GT and HBSS equally preserve the viability of PDLs in 1, 3, and 15 h.[ 9 ]…”

“…An ideal storage medium should keep alive and functional the PDL cells. [ 9 ] For this purpose, a medium with physiologic osmolality and pH, containing essential nutrients for cell growth is needed that is also easily available. [ 9 ]…”

“…[ 13 ] These polyphenols have anti-inflammatory and antioxidant effects. [ 13 ] GTE contains some important elements for cell growth such as calcium (Ca), selenium, zinc, magnesium (Mg), iron, and fluoride as well as carbohydrates and Vitamins B, C, and E.[ 9 ] The pH and osmolality of this solution were reported lower than physiologic range. [ 9 12 ] However, it was shown that GTE in combination with low-dose cyclosporine A can prolong allograft survival in mice.…”

Assessment of the viability of human periodontal ligament cells in black tea, lime juice, and passion fruit concentrate – A comparative in vitro study

“…The cell line (L929 fibroblasts) was propagated in Dulbecco modified minimum essential medium in a humidified atmosphere of 5% CO 2 and 95% O 2 at 37°C with antibiotic and anti-mycotic solutions, to prevent contamination and were incubated for 48 h and then visualized under inverted microcope. [ 7 ]…”

Network Meta-Analysis of 10 Storage Mediums for Preserving Avulsed Teeth

“…We obtained 31 full texts to further verify their eligibility. After evaluating the full texts, 11 studies were excluded due to six reasons: ineligible topic (n = 5), no full text available (n = 1), duplicate records (n = 2), ineligible media (n = 1), ineligible outcome (n = 1), and inadequate data (n = 1), and then 20 eligible studies (6,(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35)(36)(37)(38)(39)(40)(41) including 31 reports were considered to be eligible for our inclusion criteria. The identification and selection of studies were Figure 1.…”