Comparative in vitro study of the effectiveness of Green tea extract and common storage media on periodontal ligament fibroblast viability

Abstract

Objective:Green tea extract (GTE) was shown to be effective in preserving periodontal ligament fibroblasts (PDLFs) of avulsed teeth. This study aimed at determining the potential of GTE in preserving the viability of PDLFs comparing with different storage media.Materials and Methods:Periodontal ligament cells were obtained from freshly extracted healthy impacted third molars and cultured in Dulbecco's Modified Eagle Medium (DMEM). Cell viability was determined by storing the cells in seven media; DMEM, tap water, Hank's balanced salt solution (HBSS), whole milk, hypotonic sucrose solution, GTE, and GTE + sucrose for 1, 2, 4, and 24 h at 37°C using tetrazolium salt-based colorimetric (3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyl tetrazolium bromide) assay. Statistical analysis was performed by one-way analysis of variance and post hoc tests.Results:GTE showed significantly higher protective effect than HBSS at 2, 4, and 24 h (P = 0.009, P = 0.02, P = 0.016), DMED at 2 h (P = 0.003), and milk at 4 h (P = 0.039).Conclusion:Although with undesirable osmolality and pH, GTE had a good ability in preserving the PDLFs comparing with other studied media.

Which is the most recommended medium for the storage and transport of avulsed teeth? A systematic review

“…Overall, 22 studies exclusively recommended natural products as storage media, while two studies recommended them in addition to HBSS and Ringer's lactate . A further three studies also recommend milk and HBSS along with different natural products .…”

“…Among the natural products other than milk, six studies recommended propolis, and five studies proposed coconut water as storage media for transporting avulsed teeth . There were four recommendations for green tea extract . The rest of the media from this category were recommended in either only one or two studies.…”

“…20 Sour milk should not be used as it harmful to the PDL cells. 82 Recent studies 26,28,30,32 have favored other natural media (other than milk) for storage of avulsed teeth, but the low cost, presence of nutrients, and ease of availability make milk a more practical choice than any other medium.…”

“…15,16,18,26,35 There were four recommendations for green tea extract. 17,23,30,35 The rest of the media from this category were recommended in either only one or two studies. Although natural products other than milk had the largest number of studies recommending them as a group, when the single most recommended medium was explored, the number of recommendations for any specific medium was greatly diluted.…”

Effect of cDMEM media containing Ectoine on human periodontal ligament mesenchymal stem cell survival and differentiation

“…The viability of periodontal ligament cells is essential for the clinical healing of replanted avulsed teeth. [14][15][16][17][18][19][20][21] This can be achieved by immediate replantation or by placing the tooth in a transport medium that is able to preserve the viability, mitogenicity, and clonogenic capacity of the injured periodontal ligament cells and their progenitors. 22,23 In studies regarding the storage conditions for avulsed teeth, a variety of different experimental setups have been tested to find an ideal storage medium.…”