In vitro Transformation of Hamster Kidney Cells by Human Adenovirus Type 12.

McBride, William David; Wiener, A.S.

doi:10.3181/00379727-115-29060

Cited by 65 publications

(20 citation statements)

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“…The effect of Ad 12 on DNA synthesis is quite different in hamster and CKT cells; in the former cellular DNA synthesis is induced, while in the latter its synthesis is inhibited. Hamster cells are transformed by Ad 12 in vitro [13,14]. Iwameji [5] reported that an attempt to transform CKT cells by Ad 12 was not successful.…”

Section: Discussionmentioning

confidence: 99%

Responses of Calf Kidney Cells to Human Adenovirus Type 12

Kimura

1971

Japanese Journal of Microbiology

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The process of infection of established calf kidney (CKT) cells with human adenovirus type 12 (Ad 12) was compared with that with type 5 (Ad 5). Ad 5 replicated in CKT cells but Ad 12 did not. In CKT cells infected with Ad 5, as much virion antigen was detected by complement-fixation (CF) test as from HeLa cells, and the rate of DNA synthesis increased to about 7 times that in uninfected cells. In CKT cells infected with Ad 12, production of T-antigen was detected by CF test, but neither production of virion antigen nor increase in DNA synthesis was observed. Confluent monolayers of CKT cells incorporated 3H-thymidine progressively into DNA after growth medium was replaced with maintenance medium. This increase in host cell DNA synthesis after changing media was inhibited by infection with Ad 12 or with ultraviolet-irradiated Ad 12.

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Section: Discussionmentioning

confidence: 99%

Responses of Calf Kidney Cells to Human Adenovirus Type 12

Kimura

1971

Japanese Journal of Microbiology

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“…Highly oncogenic human adenovirus type 12 (Ad12) transforms rodent cells in vitro (15). The transformed cells synthesize virus-specific RNA and virus-specific T antigen (2,16) and induce tumors in newborn rodents (8).…”

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Transforming DNA sequences in rat cells transformed by DNA fragments of highly oncogenic human adenovirus type 12

Sawada¹,

Ojima²,

Shimojo³

et al. 1979

J Virol

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Rat cell lines transformed by viral DNA fragments, EcoRI-C and HindIII-G, of adenovirus type 12 DNA were analyzed for the viral transforming DNA sequences present in cell DNAs. Cell lines transformed by the EcoRI-C fragment of adenovirus type 12 DNA (leftmost 16.5% of the viral genome) contain most of the HindIII-G sequences (leftmost 6.8% of the viral genome; the smallest DNA fragment with complete transforming ability) at two to five copies per haploid cell DNA. Cell lines transformed by the HindIII-G fragment contain nearly all of the DNA sequences of the HindIII-G fragment, but at a different frequency depending on the portions of the fragment. The sequence of the AccI-H fragment of adenovirus type 12 DNA (the left part of the HindIII-G; leftmost 4.5% of the viral genome) was detected dominantly in cells transformed by the HindIII-G fragment. Southern blot analysis showed that viral DNA sequences are present at multiple integration sites in high-molecular-weight cell DNA from cells transformed by the EcoRI-C or HindIII-G fragment of adenovirus type 12 DNA. These results suggest that most of the HindIII-G sequences in cells transformed by the HindIII-G fragment are present as fragmented forms.

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“…'-4 Primarily for this reason it has also been suspected of being involved in human neoplasia.5 In cell culture, adenoviruses have been shown to transform hamster, rat, and rabbit embryo cells. [6][7][8][9] The transformants are recognized by their altered morphology and by their ability to grow on top of one another. They form multilayered foci and can be readily distinguished from the background of untransformed cells.…”

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Human cell strains susceptible to focus formation by human adenovirus type 12.

Todaro¹,

Aaronson²

1968

Proc. Natl. Acad. Sci. U.S.A.

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Adenovirus type 12 has been shown to produce tumors when inoculated into newborn animals of various species.'-4 Primarily for this reason it has also been suspected of being involved in human neoplasia.5 In cell culture, adenoviruses have been shown to transform hamster, rat, and rabbit embryo cells.6-9 The transformants are recognized by their altered morphology and by their ability to grow on top of one another. They form multilayered foci and can be readily distinguished from the background of untransformed cells. The adenovirus type 12 tumors and transformed lines contain a virus-specific new intranuclear tumor (T) antigen'0 and virus-specific messenger RNA." The cells transformed in culture produce tumors when inoculated into the appropriate host, and these tumors continue to show the presence of virus-specific genetic and antigenic material.'The present experiments demonstrate that adenovirus type 12 is able to produce altered foci in certain "susceptible" human cell cultures. Discrete adenovirus-induced foci are first seen 10-15 days after infection. The system, using human diploid cells, provides a method of quantitating the focus-forming ability of at least one type of human adenovirus.Materials and Methods.-Cells: Human fibroblast strains were derived from embryo lung, newborn foreskin, and adult skin. The last were initiated from 4-mm skin-punch biopsies. The tissue was finely minced and inoculated into 20 cm2 plastic Petri dishes containing Dulbecco and Vogt's modification of Eagle's medium supplemented with 10% calf serum. After approximately 2 weeks there generally was sufficient growth to allow subculture. The cells were then passaged at a 1:2 to 1 :10 dilution every 4-5 days and were used in the assays before the tenth transfer generation. Skin fibroblast cultures have been obtained from normal individuals and from individuals with genetic diseases associated with an increased risk of tumor formation. In particular, cell strains have been initiated from individuals with Fanconi's anemia, an autosomal recessive disease associated with a high risk of leukemia.12 1" These strains have previously been shown to be particularly susceptible to transformation in culture by another oncogenic DNA virus, SV40.14 Virus: Adenovirus type 12 (Huie strain), titering 106 5 PFU/0.1 ml on human embryonic kidney (HEK) cells, was obtained from Dr. W. Rowe (NIH), and one pool was used in all experiments.Experimental: Previous studies with SV40 transformation of the mouse line 3T315and human diploid strains'4 have shown that the efficiency of transformation is increased if rapidly dividing cultures rather than stationary, nonproliferating cultures are infected, and also if the cells are allowed several divisions subsequent to infection before they become arrested by contact inhibition of cell division. A similar requirement for several cell divisions for the expression of transformation has been shown for adenovirus type 12 focus formation in hamster embryo cells.'6 For this reason rapidly dividing cultures were inocula...

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In vitro Transformation of Hamster Kidney Cells by Human Adenovirus Type 12.

Cited by 65 publications

References 1 publication

Responses of Calf Kidney Cells to Human Adenovirus Type 12

Responses of Calf Kidney Cells to Human Adenovirus Type 12

Transforming DNA sequences in rat cells transformed by DNA fragments of highly oncogenic human adenovirus type 12

Human cell strains susceptible to focus formation by human adenovirus type 12.

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