In vitro transcriptional probes of heterotic rat liver chromatin

Tallman, Gary; Butcher, D.F.; Kaczmarczyk, Walter; Ulrich, V.

doi:10.1093/oxfordjournals.jhered.a108872

Cited by 5 publications

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“…Biochemical studies of hybrids during development reveal that some parameters are correlated with heterosis and others are not. The liver chromatin of heterotic rats manifested no consistent correlation in the RNA/DNA, nonhistone/DNA and histone/DNA ratios (Tallman et al 1977(Tallman et al , 1978(Tallman et al , 1979. Although nonhistone electrophoretic patterns of inbreds and hybrids were swain-specific less variation was observed in the patterns of the hybrid (Tallman et al 1979).…”

Section: Introductionmentioning

confidence: 88%

Nuclease accessibility of chromatin from a heterotic hybrid and from parental inbreds

Palmer¹,

Ulrich²

1992

Biol. plant.

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The DNAase II, Mg 2+ procedure was used to fractionate the seedling chromatin of a heterotic maize hybrid and its parental inbreds FRM017 and FRN28. The hybrid and the more vigorous maize inbred FRN28 have 14 and I 1 percent soluble chromatin (euchromatin) respectively, while the less vigorous FRM017 contains 30 percent. The unfractionated chromatin of the hybrid contains less protein than either inbred. The RNA contents of the unfractionated chromatin of the hybrid and of FRN28 are similar and are one-half that of less vigorous FRM017. Hybrid euchromatin contains relatively more protein and RNA than DNA as well as higher proportions of protein and RNA than heterochromatin, unfractionated chromatin, or inbred euchromatin; this suggests a more "efficient" type of activity, as reflected by the low amount of euchromatin and a high proportion of RNA and chromosomal proteins.

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Section: Introductionmentioning

confidence: 88%

Nuclease accessibility of chromatin from a heterotic hybrid and from parental inbreds

Palmer¹,

Ulrich²

1992

Biol. plant.

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Comparisons of liver chromatin proteins and template activities in parental and heterotic rats during postweaned development

Amero

Kaczmarczyk

et al. 1983

Biochem Genet

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Electrophoretic profiles of acid-extractable proteins from Holtzman rat liver chromatin display four minor and five major histone bands through certain stages of postweaned development but are qualitatively different from the chromatin protein profiles previously reported during postweaned development for the Fisher 344 rat strain and the F344 X Holtzman heterotic progeny [Tallman, G., et al. (1979). Biochem, Genet. 17:185]. The protein profiles from the heterotic progeny do not reflect and are not combinations of the profiles from the parental strains. Levels of in vitro transcription with Escherichia coli RNA polymerase of total chromatin and of acid-extracted chromatin from Holtzman rat liver tissue fluctuate in an age-specific manner during postweaned development and are higher than previously published levels determined for the Fisher and F344 X H strains during the same developmental period [Tallman, G., et al. (1978). J. Hered. 69:282]. The degrees of stimulation in the transcription assays resulting from the acid treatment vary with the age of the animal but are similar for the maternal Holtzman and hybrid strains. These studies suggest that regulation of heterotic growth may involve dominant, or maternal genetic influences.

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Electrophoretic comparisons of liver chromatin proteins isolated from heterotic rats during postweaning development

et al. 1979

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Electrophoretic comparisons of histones and other acid-extractable chromatin proteins isolated from the livers of growing F344 inbred rats and a heterotic paternal hybrid derived by crossing F344 males with Holtzman females reveal significant heterogeneity among a class of nonhistone components soluble in acid. These variations appear to be age and line specific and show more variation in the inbred than the hybrid. Comparisons of the acid-insoluble nonhistone proteins by sodium dodecylsulfate gel electrophoresis reveal significant quantitative changes in a 28,600 dalton polypeptide present in large quantities at 30, 35, and 50 days of age in the hybrid and at 40 and 45 days only in the inbred. Other minor variations were noted in a class of proteins of 40,000--45,000 molecular weight and in those of very high molecular weight (100,000-200,000). Such variations could be a reflection of or prelude to changes in genetic activity and could ultimately be important in the control of growth patterns of developing heterotic animals.

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In vitro transcriptional probes of heterotic rat liver chromatin

Cited by 5 publications

References 0 publications

Nuclease accessibility of chromatin from a heterotic hybrid and from parental inbreds

Nuclease accessibility of chromatin from a heterotic hybrid and from parental inbreds

Comparisons of liver chromatin proteins and template activities in parental and heterotic rats during postweaned development

Electrophoretic comparisons of liver chromatin proteins isolated from heterotic rats during postweaning development

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