Electrophoretic comparisons of liver chromatin proteins isolated from heterotic rats during postweaning development

Abstract

Electrophoretic comparisons of histones and other acid-extractable chromatin proteins isolated from the livers of growing F344 inbred rats and a heterotic paternal hybrid derived by crossing F344 males with Holtzman females reveal significant heterogeneity among a class of nonhistone components soluble in acid. These variations appear to be age and line specific and show more variation in the inbred than the hybrid. Comparisons of the acid-insoluble nonhistone proteins by sodium dodecylsulfate gel electrophoresis reveal significant quantitative changes in a 28,600 dalton polypeptide present in large quantities at 30, 35, and 50 days of age in the hybrid and at 40 and 45 days only in the inbred. Other minor variations were noted in a class of proteins of 40,000--45,000 molecular weight and in those of very high molecular weight (100,000-200,000). Such variations could be a reflection of or prelude to changes in genetic activity and could ultimately be important in the control of growth patterns of developing heterotic animals.

The nuclear proteins in the liver of rabbits with an inherited motorial failure

“…The changes in the composition of the liver nuclear proteins observed in our experiment may also be elucidated by genetical differences between rabbits with an inborn defect and normal ones. As it is known, the content and the electrophoretic patterns of nonhistone proteins depend not only on the species (C hiu and H nilica 1978) but also on the breed and genetical line of the animals (T allman et al 1979; S arsenov 1981). Similar species and intraspecies differences were also demonstrated in the case of histones, especially H1 histone in insects, fish and birds (B erdnikov 1981; Z alensky et al 1981; M artinez and McD aniel , 1981) and H2A histone in the sperm of sea urchin (D e P etrocellis et al 1980; S trickland et al 1981).…”

“…It is known that nuclear proteins play an important role in the maintenance of the structural and functional organization of the eukaryotic genome. The composition of those proteins changes according to the genotype (T allman et al 1979; B erdnikov 1981; L uboń et al 1982) and the functional condition of cells and tissues (S ziklai et al 1981; R avi and G anguli 1980).…”

Nuclease accessibility of chromatin from a heterotic hybrid and from parental inbreds

“…Biochemical studies of hybrids during development reveal that some parameters are correlated with heterosis and others are not. The liver chromatin of heterotic rats manifested no consistent correlation in the RNA/DNA, nonhistone/DNA and histone/DNA ratios (Tallman et al 1977(Tallman et al , 1978(Tallman et al , 1979. Although nonhistone electrophoretic patterns of inbreds and hybrids were swain-specific less variation was observed in the patterns of the hybrid (Tallman et al 1979).…”

“…The liver chromatin of heterotic rats manifested no consistent correlation in the RNA/DNA, nonhistone/DNA and histone/DNA ratios (Tallman et al 1977(Tallman et al , 1978(Tallman et al , 1979. Although nonhistone electrophoretic patterns of inbreds and hybrids were swain-specific less variation was observed in the patterns of the hybrid (Tallman et al 1979). Chromatographer hybrid histone H1 showed quantitative and qualitative variation over development, but when electrophoresed, inbred H1 patterns were more variable (Tallmann et al 1978, Amero et al 1983.…”