In vitro Trans Translation mediated by alanine-charged 10sa RNA

Himeno, Hyouta; Sato, Masakazu; Tadaki, Toshimasa; Fukushima, Masaaki; Ushida, Chisato; Muto, Akira

doi:10.1006/jmbi.1997.1011

Cited by 86 publications

(90 citation statements)

References 26 publications

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“…As shown earlier (19,29), amino acids were incorporated in a stoichiometrical fashion in the absence of paromomycin (Fig. 3a).…”

Section: Various Effects Of Paromomycin On Trans-translationsupporting

confidence: 77%

“…This would relieve stalled translation from mRNAs lacking a stop codon or possessing a cluster of rare codons with the addition of a specific tag peptide as a degradation signal to the truncated C termini of the polypeptides decoded (4,7,17,18). This trans-translation model has also been supported by several other findings showing that the function as a tRNA is a prerequisite for the function as an mRNA in vitro (19), that an amino acid aminoacylated to tmRNA is actually incorporated into the tag peptide (20), and that tmRNA binds predominantly to 70 S ribosomes (4,21,22). A series of these processes may promote recycling of ribosomes and prevent accumulation of abortively synthesized polypeptides, providing some advantage to the cell for survival (16,23,24).…”

supporting

confidence: 60%

“…This mutant has no alanine acceptor activity because of the lack of an identity determinant for recognition by alanyl-tRNA synthetase, thereby having no trans-translation activity (19). At any concentration of paromomycin, no detectable incorporations of amino acids other than phenylalanine were observed in the 3A mutant.…”

Section: Exogenous Addition Of Smpb Enhanced the Initiation-shifted Tmentioning

confidence: 96%

“…It interacts with G 19 and A 334 or their surroundings in the lower portion of the tRNA domain of tmRNA to enhance aminoacylation efficiency, protect tmRNA from degradation in the cells, and mediate ribosome binding to tmRNA (30,35). The binding sites of SmpB and paromomycin appear to be close to each other.…”

Section: Effects Of Paromomycin On Aminoacylation Of Tmrna In Thementioning

confidence: 99%

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Various Effects of Paromomycin on tmRNA-directed trans-Translation

Takahashi

Konno

Muto

et al. 2003

Journal of Biological Chemistry

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“…As shown earlier (19,29), amino acids were incorporated in a stoichiometrical fashion in the absence of paromomycin (Fig. 3a).…”

Section: Various Effects Of Paromomycin On Trans-translationsupporting

confidence: 77%

supporting

confidence: 60%

Section: Exogenous Addition Of Smpb Enhanced the Initiation-shifted Tmentioning

confidence: 96%

Section: Effects Of Paromomycin On Aminoacylation Of Tmrna In Thementioning

confidence: 99%

See 2 more Smart Citations

Various Effects of Paromomycin on tmRNA-directed trans-Translation

Takahashi

Konno

Muto

et al. 2003

Journal of Biological Chemistry

Self Cite

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“…Himeno et al, 1997;Williams and Bartel, 1998;Zweib et al, 1998). It is charged with alanine (Komine et al, 1994;Ushida et al, 1994) and also has a short reading frame coding for 10 amino acids (Tu et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

Presence and location of modified nucleotides in Escherichia coli tmRNA: structural mimicry with tRNA acceptor branches

et al. 1998

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Escherichia coli tmRNA functions uniquely as both tRNA and mRNA and possesses structural elements similar to canonical tRNAs. To test whether this mimicry extends to post-transcriptional modification, the technique of combined liquid chromatography/ electrospray ionization mass spectrometry (LC/ESIMS) and sequence data were used to determine the molecular masses of all oligonucleotides produced by RNase T 1 hydrolysis with a mean error of 0.1 Da. Thus, this allowed for the detection, chemical characterization and sequence placement of modified nucleotides which produced a change in mass. Also, chemical modifications were used to locate mass-silent modifications. The native E.coli tmRNA contains two modified nucleosides, 5-methyluridine and pseudouridine. Both modifications are located within the proposed tRNAlike domain, in a seven-nucleotide loop mimicking the conserved sequence of T loops in canonical tRNAs. Although tmRNA acceptor branches (acceptor stem and T stem-loop) utilize different architectural rules than those of canonical tRNAs, their conformations in solution may be very similar. A comparative structural and functional analysis of unmodified tmRNA made by in vitro transcription and native E.coli tmRNA suggests that one or both of these post-transcriptional modifications may be required for optimal stability of the acceptor branch which is needed for efficient aminoacylation.

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Proteostasis regulation through ribosome quality control and no‐go‐decay

et al. 2023

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Cell functionality relies on the existing pool of proteins and their folding into functional conformations. This is achieved through the regulation of protein synthesis, which requires error‐free mRNAs and ribosomes. Ribosomes are quality control hubs for mRNAs and proteins. Problems during translation elongation slow down the decoding rate, leading to ribosome halting and the eventual collision with the next ribosome. Collided ribosomes form a specific disome structure recognized and solved by ribosome quality control (RQC) mechanisms. RQC pathways orchestrate the degradation of the problematic mRNA by no‐go decay and the truncated nascent peptide, the repression of translation initiation, and the recycling of the stalled ribosomes. All these events maintain protein homeostasis and return valuable ribosomes to translation. As such, cell homeostasis and function are maintained at the mRNA level by preventing the production of aberrant or unnecessary proteins. It is becoming evident that the crosstalk between RQC and the protein homeostasis network is vital for cell function, as the absence of RQC components leads to the activation of stress response and neurodegenerative diseases. Here, we review the molecular events of RQC discovered through well‐designed stalling reporters. Given the impact of RQC in proteostasis, we discuss the relevance of identifying endogenous mRNA regulated by RQC and their preservation in stress conditions.This article is categorized under: RNA Turnover and Surveillance > Turnover/Surveillance Mechanisms Translation > Regulation

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In vitro Trans Translation mediated by alanine-charged 10sa RNA

Cited by 86 publications

References 26 publications

Various Effects of Paromomycin on tmRNA-directed trans-Translation

Various Effects of Paromomycin on tmRNA-directed trans-Translation

Presence and location of modified nucleotides in Escherichia coli tmRNA: structural mimicry with tRNA acceptor branches

Proteostasis regulation through ribosome quality control and no‐go‐decay

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