In vitro synthesis of late bacteriophage phi 29 RNA

Abstract

A crude P-100 fraction prepared from Bacillus subtilis 21 min after infection with wild-type phage 4)29 supported the in vitro synthesis of late 4)29 RNA by added RNA polymerase. Synthesis of late RNA was also detected when purified 4)29 DNA was transcribed by RNA polymerase in the presence of an S-150 fraction obtained by lysis of 4)29-infected cells in the presence of 1 M NaCl. Late 4)29 RNA was not synthesized when either the P-100 or the S-150 fraction was prepared from cultures infected with 4)29 having a mutation in gene 4.