Effect of ultraviolet radiation on the Bacillus subtilis phages SPO2, SPP1 and φ29 and their DNAs

Freeman, Abbie G.; Schweikart, Karen; Larcom, Lyndon L.

doi:10.1016/0167-8817(87)90016-2

Cited by 3 publications

(2 citation statements)

References 59 publications

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“…The inactivation curve for PCV-2 demonstrated that UV-C radiation inactivated this virus; however, even at the highest dose (9000 J/L), residual levels of PCV-2 were recovered. The presence of a resistant viral subpopulation has been attributed to the ability of some viruses to take advantage of host cell repair mechanisms [52], or in some cases they can code for their own repair machinery [53]. It has also been argued that viral particle clumping, particularly with other cells or debris, may shield some virus from UV-C radiation [50].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the effectiveness of the SurePure Turbulator ultraviolet-C irradiation equipment on inactivation of different enveloped and non-enveloped viruses inoculated in commercially collected liquid animal plasma

et al. 2019

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The objective of this study was to evaluate the effectiveness of the SurePure Turbulator ultraviolet-C (UV-C, 254 nm wavelength) irradiation equipment on inactivation of different enveloped and non-enveloped viruses in commercially collected liquid animal plasma. Specifically, Pseudorabies virus (PRV), Porcine reproductive and respiratory syndrome virus (PRRSV), Porcine epidemic diarrhea virus (PEDV), Bovine viral diarrhea virus (BVDV), Classical swine fever virus (CSFV), Swine influenza virus (SIV) as enveloped viruses and Porcine parvovirus (PPV), Swine vesicular disease virus (SVDV), Porcine circovirus type 2 (PCV-2) and Senecavirus A (SVA) as non-enveloped viruses, were inoculated in bovine or porcine plasma and subjected to different UV-C irradiation doses (0, 750, 1500, 3000, 6000 and 9000 J/L) using an UV-C device developed for opaque liquid working under turbulent flow. The enveloped viruses tested were inactivated at < 3000 J/L of UV-C, being the dose needed to inactivate 4 log TCID 50 (4D) of 1612 J/L for PRV,1004 J/L for PRRSV, 1953 J/L for PEDV, 1639 J/L for SIV, 1641 J/L for CSFV and 1943 J/L for BVDV. The non-enveloped viruses tended to have higher 4D values: 2161 J/L for PPV, 3223 J/L for SVA and 3708 J/L for SVDV. Because the initial viral concentration was <4.0 Log for PCV-2, it was not possible to calculate the 4D value for this virus. In conclusion, these results demonstrated that the SurePure Turbulator UV-C treatment system is capable of inactivating significant levels of swine viruses inoculated in commercially collected porcine or bovine plasma. It was concluded that irradiation with UV-C can provide an additional redundant biosafety feature in the manufacturing process of spray-dried animal plasma.

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Section: Discussionmentioning

confidence: 99%

Evaluation of the effectiveness of the SurePure Turbulator ultraviolet-C irradiation equipment on inactivation of different enveloped and non-enveloped viruses inoculated in commercially collected liquid animal plasma

et al. 2019

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show abstract

“…The discrepancies in results also demonstrate the complexities associated with studies to evaluate UVC inactivation of viruses, as the outcome of such experiments is dependent on the cell systems used to assay UVC-damaged viruses. For example, some viruses may experience UVC-induced DNA damage but appear UVCresistant because they code for their own repair enzymes [22] or take advantage of host cell repair mechanisms [23]. As PCR is less prone to the variations associated with cell-based assays, and PCR assay conditions are relatively easy to define and control, PCR may be a more reliable method for using to analyse and compare the UVC results from different laboratories.…”

Section: Figmentioning

confidence: 99%