Evaluation of the effectiveness of the SurePure Turbulator ultraviolet-C irradiation equipment on inactivation of different enveloped and non-enveloped viruses inoculated in commercially collected liquid animal plasma

Blázquez, Elena; Rodrı́guez, Carmen; Ródenas, Jesús; Navarro, Núria; Riquelme, Cristina; Rosell, Rosa; Campbell, Joy; Crenshaw, J. D.; Segalés, Joaquím; Pujols, Joan; Polo, Javier

doi:10.1371/journal.pone.0212332

Cited by 38 publications

(44 citation statements)

References 56 publications

(83 reference statements)

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“…The World Health Organization (WHO) (WHO, 2004) guidelines on viral inactivation and removal procedures intended to assure the viral safety of human blood plasma products establishes that a robust inactivation process should be capable of removing or inactivating at least 4 logs of a wide range of viruses (WHO, 2004). In previous studies, the UV-C irradiation of liquid plasma was able to inactive 4 logs of different bacteria and viruses (Polo et al, 2015;Blázquez et al, 2017Blázquez et al, , 2019 and the present study also demonstrated the lack of transmission of any virus after being UV irradiated at a minimum of 3000 J/L. Therefore, UV-C irradiation complies with the WHO definition of a robust inactivation process.…”

Section: Discussionsupporting

confidence: 83%

UV-C irradiation is able to inactivate pathogens found in commercially collected porcine plasma as demonstrated by swine bioassay

Blázquez¹,

Rodrı́guez²,

Ródenas³

et al. 2019

Veterinary Microbiology

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Section: Discussionsupporting

confidence: 83%

UV-C irradiation is able to inactivate pathogens found in commercially collected porcine plasma as demonstrated by swine bioassay

Blázquez¹,

Rodrı́guez²,

Ródenas³

et al. 2019

Veterinary Microbiology

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“…To validate the effectiveness of the plasma UV-C irradiation measured by means of the viral load reduction in cell culture, a bioassay was done with different groups of pigs injected intraperitoneally with UV-C irradiated commercial liquid plasma at 0 J/L (untreated plasma), 3000 J/L, and at 9000 J/L [77]. The results of the bioassay showed that none of the pigs in the groups that received liquid plasma irradiated by UV-C at either dose became infected or seroconverted against the different virus genomes that were detected in the initial plasma (PCV-2, PRRSV (European strains), SIV, PPV, HEV, Rotavirus A (RVA); thus, confirming the efficacy of UV-C demonstrated in vitro in a previous study [74]. Detection of a viral genome in the untreated liquid plasma does not imply infectivity by a given virus.…”

Section: Uv Irradiation Effect On Bacteria Survival In Sdpsupporting

confidence: 85%

“…The effect of UV-C on different viruses of interest in the swine industry was also determined [74]. Viruses selected for testing was based on their different physicochemical characteristics, types of genome (DNA or RNA), genome lengths (long and short genomes), presence or absence of envelope and resistance to other inactivation processes.…”

Section: Uv Irradiation Effect On Bacteria Survival In Sdpmentioning

confidence: 99%

Biosafety steps in the manufacturing process of spray-dried plasma: a review with emphasis on the use of ultraviolet irradiation as a redundant biosafety procedure

et al. 2020

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Spray dried plasma (SDP) is a functional protein source obtained from blood of healthy animals, approved by the veterinary authorities from animals declared to be fit for slaughter for human consumption. Blood of these animals is collected at the slaughterhouse, treated with an anticoagulant, chilled and transported to industrial facilities in which blood is centrifuged to separate the red blood cells from the plasma fraction. Plasma is then concentrated, and spray dried at high temperatures (80°C throughout its substance) to convert it in a powder. Such method preserves the biological activity of its proteins, mainly albumins and globulins. SDP is mainly used in pig feed diets to significantly improve daily gain, feed intake, production efficiency, and to reduce post-weaning lag caused by the appearance of post-weaning diarrhea. Although SDP is considered a safe product and its manufacturing process consists of several biosafety steps, the security of the SDP is often questioned due to its nature as raw blood by-product, especially when emergent or re-emergent pathogens appear. This review provides an evaluation and validation of the different safety steps present in the manufacturing process of SDP, with special focus on a new redundant pathogen inactivation step, the UV-C irradiation, that may be implemented in the manufacturing process of the SDP. Overall results showed that the manufacturing process of SDP is safe and the UV-C radiation was effective in inactivating a wide range of bacteria and viruses spiked and naturally present in commercially collected liquid animal plasma and it can be implemented as a redundant biosafety step in the manufacturing process of the SDP. Background Spray-dried plasma (SDP) is a functional protein source obtained from blood of healthy animals approved to be sacrificed for human consumption after veterinary inspection. Blood of these animals is collected at the slaughterhouse, treated with an anticoagulant, chilled and transported to industrial facilities where the blood is centrifuged to separate the red blood cells (RBC) from the plasma fraction. Alternatively, the blood may be centrifuged in the abattoir and then the chilled plasma transported to the manufacturing plant. Plasma is subsequently concentrated either by membrane filtration or vacuum evaporation and spray-dried at high temperatures (80°C throughout its substance) to convert it to powder. This process preserves the biological activity of

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“…We instead applied multiple categorical predictors. With inactivation of viruses in blood products without describing how attenuation was considered in their reported doses (10,(56)(57)(58). Although these doses are likely representative for these fluids, they cannot be extrapolated to other matrices.…”

Section: Numerous Uv254 Rate Constants Are Available But Only For a mentioning

confidence: 99%

Predictive modeling of virus inactivation by UV

Rockey

Henderson

Chin

et al. 2020

Preprint

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Disinfection strategies are commonly applied to inactivate pathogenic viruses in water, food, air, and on surfaces to prevent the spread of infectious diseases. Determining how quickly viruses are inactivated to mitigate health risks is not always feasible due to biosafety restrictions or difficulties with virus culturability. Therefore, methods that would rapidly predict kinetics of virus inactivation by UV254 would be valuable, particularly for emerging and difficult-to-culture viruses. We conducted a rapid systematic literature review to collect high-quality inactivation rate constants for a wide range of viruses. Using these data and basic virus information (e.g., genome sequence attributes), we developed and evaluated four different model classes, including linear and non-linear approaches, to find the top performing prediction model. For both the (+) ssRNA and dsDNA virus types, multiple linear regressions were the top performing model classes. In both cases, the cross-validated root mean squared relative prediction errors were similar to those associated with experimental rate constants. We tested the models by predicting and measuring inactivation rate constants for two viruses that were not identified in our systematic review, including a (+) ssRNA mouse coronavirus and a dsDNA marine bacteriophage; the predicted rate constants were within 7% and 71% of the experimental rate constants, respectively. Finally, we applied our models to predict the UV254 rate constants of several viruses for which high-quality UV254 inactivation data are not available. Our models will be valuable for predicting inactivation kinetics of emerging or difficult-to-culture viruses.

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Evaluation of the effectiveness of the SurePure Turbulator ultraviolet-C irradiation equipment on inactivation of different enveloped and non-enveloped viruses inoculated in commercially collected liquid animal plasma

Cited by 38 publications

References 56 publications

UV-C irradiation is able to inactivate pathogens found in commercially collected porcine plasma as demonstrated by swine bioassay

UV-C irradiation is able to inactivate pathogens found in commercially collected porcine plasma as demonstrated by swine bioassay

Biosafety steps in the manufacturing process of spray-dried plasma: a review with emphasis on the use of ultraviolet irradiation as a redundant biosafety procedure

Predictive modeling of virus inactivation by UV

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