In vitro synthesis of aortic glycosaminoglycans effect of diet, lipoproteins and diabetes

Telner, Adam; Kalant, N.

doi:10.1016/0021-9150(74)90081-1

Cited by 6 publications

(2 citation statements)

References 26 publications

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“…Disturbances in the intercellular matrix have been described in various types of diabetes at the level of glycosaminoglycans (Berenson et al, 1972;Malathy & Kurup, 1972;Telner & Kalant, 1974;Kern et al, 1976b), glycoproteins (Kern et al, 1972(Kern et al, , 1976aRobert etal., 1972), collagens (Berenson etal., 1972;Ramamurthy et al, 1972;Kern et al, 1972Kern et al, , 1976aFrancis et al, 1974) and basement membranes (Spiro, 1973;Kefalides, 1974;Westberg, 1976;Beisswenger, 1976;Ristelli et al, 1976). Alterations of the cells synthesizing these macromolecules have also been demonstrated in diabetic subjects (Goldstein etal., 1969; Vracko & Benditt, 1975).…”

Section: Discussionmentioning

confidence: 99%

“…We have postulated that alterations of collagen and/or glycoprotein components of the basement membranes would be only one aspect of a general disturbance of the regulation of intercellular-matrix biosynthesis in diabetes (Robert, 1971;Kern et al, 1972). Several authors and ourselves have shown qualitative and quantitative alterations at the levels of collagens (Berenson et al, 1972;Ramamurthy et al, 1972;Francis et al, 1974;Kern et al, 1976a), glycosaminoglycans and glycoproteins (Berenson et al, 1972;Kern et al, 1972;Malathy & Kurup, 1972;Robert et al, 1972;Telner & Kalant, 1974;Kern et al, 1976b) in several connective tissues and in various types of diabetes.…”

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confidence: 98%

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Biosynthesis of skin collagens in normal and diabetic mice

Kern¹,

Moczar²,

Robert³

1979

Biochemical Journal

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Synthesis of collagens in vitro was studied on minced mouse skins incubated with [3H]-proline in organ-culture conditions. A comparative study was carried out on genetically diabetic mice (KK strain) and control mice (Swiss strain). After incubation, neutral-salt-soluble and acid-soluble collagens were extracted. The insoluble dermis was digested by pepsin and type I and type III collagens separated by differential precipitation in neutral salt solutions. Type I and Type III collagens were characterized by ion-exchange and molecular-sieve chromatography, amino acid analysis and by the characterization of CNBr peptides. In diabetic-mouse skin, the relative proportion of type III collagen was significantly higher than in control-mouse skin. The incorporation of radioactively labelled proline into hydroxyproline of type III collagen was significantly faster in diabetic-mouse skin than in control-mouse skin. No significant modifications in the total collagen content of the skin or of their rates of synthesis were observed between the two strains. Alteration in the ratio of type III to type I collagen in the diabetic-mouse skin can be interpreted as a sign of alteration of the regulation of collagen biosynthesis and may be related to the structural alterations observed in the diabetic intercellular matrix.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 98%

Biosynthesis of skin collagens in normal and diabetic mice

Kern¹,

Moczar²,

Robert³

1979

Biochemical Journal

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Interaction of Low Density Lipoproteins with Arterial Constituents: Its Relationship with Atherogenesis

Camejo

1976

Low Density Lipoproteins

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Influence of streptozotocin- and alloxan-induced diabetes on the metabolism of glycosaminoglycans

Mohanam

Bose

1984

Acta diabet. lat

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GAG metabolism was investigated in rats with experimentally induced diabetes. In comparison to control animals, the uptake of 35S-sulfate was diminished in tissues of diabetic animals. Streptozotocin-induced diabetes showed a significant decrease in the content of GAG fractions except that of non-sulfated GAG in liver and kidney which was unchanged as compared to the control group. In rats rendered diabetic by alloxan, non-sulfated GAG increased appreciably in liver and kidney whereas highly sulfated GAG remained unchanged. In the skins of alloxan-diabetic rats both total and sulfated GAG decreased significantly. The activities of liver beta-glucuronidase, beta-N-acetyl glucosaminidase and cathepsin D were significantly increased in rats treated with streptozotocin and alloxan. In streptozotocin-diabetic rats, renal beta-glucuronidase and beta-N-acetyl glucosaminidase activities were reduced while cathepsin D activity was similar to that of controls. The renal beta-N-acetyl glucosaminidase and cathepsin D activities of alloxan-treated rats were not significantly different from normal but their beta-glucuronidase was significantly increased. In the spleen of streptozotocin-diabetic rats all the enzymes were increased except beta-N-acetyl glucosaminidase which remained unaltered. Increased excretion of uronic acid was observed in diabetic groups. These results collectively indicate that both streptozotocin- and alloxan-induced diabetes altered the synthesis and catabolism of GAG.

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In vitro synthesis of aortic glycosaminoglycans effect of diet, lipoproteins and diabetes

Cited by 6 publications

References 26 publications

Biosynthesis of skin collagens in normal and diabetic mice

Biosynthesis of skin collagens in normal and diabetic mice

Interaction of Low Density Lipoproteins with Arterial Constituents: Its Relationship with Atherogenesis

Influence of streptozotocin- and alloxan-induced diabetes on the metabolism of glycosaminoglycans

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