In vitro synergic activity of diethyldithiocarbamate and 4-nitrochalcone loaded in beeswax nanoparticles against melanoma (B16F10) cells

Cordeiro, Arthur Poester; Feuser, Paulo Emílio; Figueiredo, Pedro Graupen; Cunha, Elizabeth Sousa da; Martinez, Gláucia Regina; Machado-de-Ávila, Ricardo Andrez; Rocha, Maria Eliane Merlin; Araújo, Pedro Henrique Hermes de; Sayer, Cláudia

doi:10.1016/j.msec.2020.111651

Cited by 7 publications

(3 citation statements)

References 45 publications

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“…In 1128 and 2063 cm −1 , we can observe the stretching C=S and, between 600 and 1100 cm −1 , we can observe the stretching C–S, C–C, C–N, CH 2 , and CH 3 . A region below 600 cm −1 corresponds to altered torsions [ 23 , 24 , 25 ]. The physical mixture presented important differences compared with NPD, mainly the intensity of the bands and the bathochromic shift of the C=O bond of the carbonyl of ester band and the overlapping by DETC bands that occurred in the physical mixture.…”

Section: Resultsmentioning

confidence: 99%

“…Cordeiro et al (2021) showed the FTIR spectrum of the free DETC, and the free DETC spectra were similar to this study, with characteristic peaks at 837, 909, and 985 cm −1 , representing the C-S bond, 1070 and 2104 for C=S, and also 1423 cm −1 for N–CS 2. Furthermore, DETC was co-encapsulated with 4-nitrohalcone in beeswax nanoparticles, and a greater intensity in the peaks of these compounds was observed according to the amount present in the formulation [ 25 ] . Furthermore, the same peaks in the FTIR were observed by Mazur et al when developing nanoemulsions of DETC and beeswax, but they were less intense than our findings because they used a shorter concentration of DETC than in this experiment [ 31 ].…”

Section: Discussionmentioning

confidence: 99%

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In Vitro Validation of Antiparasitic Activity of PLA-Nanoparticles of Sodium Diethyldithiocarbamate against Trypanosoma cruzi

et al. 2022

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Trypanosoma cruzi is a protozoan parasite responsible for Chagas disease, which affects millions around the world and is not treatable in its chronic stage. Sodium diethyldithiocarbamate is a compound belonging to the carbamate class and, in a previous study, demonstrated high efficacy against T. cruzi, showing itself to be a promising compound for the treatment of Chagas disease. This study investigates the encapsulation of sodium diethyldithiocarbamate by poly-lactic acid in nanoparticles, a system of biodegradable nanoparticles that is capable of reducing the toxicity caused by free DETC against cells and maintaining the antiparasitic activity. The nanosystem PLA-DETC was fabricated using nanoprecipitation, and its physical characterization was measured via DLS, SEM, and AFM, demonstrating a small size around 168 nm and a zeta potential of around −19 mv. Furthermore, the toxicity was determined by MTT reduction against three cell lines (VERO, 3T3, and RAW), and when compared to free DETC, we observed a reduction in cell mortality, demonstrating the importance of DETC nanoencapsulation. In addition, the nanoparticles were stained with FITC and put in contact with cells for 24 h, followed by confirmation of whether the nanosystem was inside the cells. Lastly, the antiparasitic activity against different strains of T. cruzi in trypomastigote forms was determined by resazurin reduction and ROS production, which demonstrated high efficacy towards T. cruzi equal to that of free DETC.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

In Vitro Validation of Antiparasitic Activity of PLA-Nanoparticles of Sodium Diethyldithiocarbamate against Trypanosoma cruzi

et al. 2022

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“…Recently Cordeiro and collaborators provided to the simultaneous encapsulation of a hydrophilic drug, sodium diethyldithiocarbamate (DETC), and a hydrophobic drug, 4-nitrochalcone (4NC), in beeswax nanoparticles (BNs) with the aim to evaluate the in vitro synergic activity of these drugs against melanoma (B16F10) cells [ 55 ]. So BNs were prepared by water/oil/water double emulsion in the absence of organic solvents.…”

Section: Nanoparticles Useful For Melanoma Treatmentmentioning

confidence: 99%

Recent Advances in Nanotechnology for the Treatment of Melanoma

et al. 2021

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Melanoma is one of the most aggressive forms of skin cancer, with few possibilities for therapeutic approaches, due to its multi-drug resistance and, consequently, low survival rate for patients. Conventional therapies for treatment melanoma include radiotherapy, chemotherapy, targeted therapy, and immunotherapy, which have various side effects. For this reason, in recent years, pharmaceutical and biomedical research has focused on new sito-specific alternative therapeutic strategies. In this regard, nanotechnology offers numerous benefits which could improve the life expectancy of melanoma patients with very low adverse effects. This review aims to examine the latest advances in nanotechnology as an innovative strategy for treating melanoma. In particular, the use of different types of nanoparticles, such as vesicles, polymers, metal-based, carbon nanotubes, dendrimers, solid lipid, microneedles, and their combination with immunotherapies and vaccines will be discussed.

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Temozolomide associated to gold nanoparticles promoted a synergic effect and apoptosis when exposed to melanoma cells

et al. 2022

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In vitro synergic activity of diethyldithiocarbamate and 4-nitrochalcone loaded in beeswax nanoparticles against melanoma (B16F10) cells

Cited by 7 publications

References 45 publications

In Vitro Validation of Antiparasitic Activity of PLA-Nanoparticles of Sodium Diethyldithiocarbamate against Trypanosoma cruzi

In Vitro Validation of Antiparasitic Activity of PLA-Nanoparticles of Sodium Diethyldithiocarbamate against Trypanosoma cruzi

Recent Advances in Nanotechnology for the Treatment of Melanoma

Temozolomide associated to gold nanoparticles promoted a synergic effect and apoptosis when exposed to melanoma cells

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