In Vitro susceptibility ofHelicobacter pylori to botanical extracts used traditionally for the treatment of gastrointestinal disorders

Abstract

The gram-negative bacterium Helicobacter pylori (HP), identified in 1982, is now recognized as the primary etiological factor associated with the development of gastritis and peptic ulcer disease. In addition, HP infections are also associated with chronic gastritis, gastric carcinoma and primary gastric B-cell lymphoma. For centuries, herbals have been used in traditional medicine to treat a wide range of ailments, including gastrointestinal (GI) disorders such as dyspepsia, gastritis and peptic ulcer disease (PUD). However, the mechanism of action by which these botanicals exert their therapeutic effects has not been completely elucidated. As part of an ongoing screening program, the study assessed the in vitro susceptibility of 15 HP strains to botanical extracts, which have a history of traditional use in the treatment of GI disorders. Methanol extracts of Myristica fragrans (seed) had a MIC of 12.5 microg/mL; Zingiber officinale (ginger rhizome/root) and Rosmarinus officinalis (rosemary leaf) had an MIC of 25 microg/mL. Methanol extracts of botanicals with a MIC of 50 microg/mL included Achillea millefolium, Foeniculum vulgare (seed), Passiflora incarnata (herb), Origanum majorana (herb) and a (1:1) combination of Curcuma longa (root) and ginger rhizome. Botanical extracts with a MIC of 100 microg/mL included Carum carvi (seed), Elettaria cardamomum (seed), Gentiana lutea (roots), Juniper communis (berry), Lavandula angustifolia (flowers), Melissa officinalis (leaves), Mentha piperita (leaves) and Pimpinella anisum (seed). Methanol extracts of Matricaria recutita (flowers) and Ginkgo biloba (leaves) had a MIC > 100 microg/mL.

Bioactivity and potential therapeutic benefits of some medicinal plants from the Caatinga (semi-arid) vegetation of Northeast Brazil: a review of the literature

“…It was found that gingerol inhibited the growth of H. pylori strains in vitro and this activity may contribute to its chemopreventive effects against colon cancer (Mahady et al, 2003). This anti-H. pylori action was further confirmed by Mahady et al (2005) and Nostro et al (2006). Recently, Weng et al (2010) demonstrated that [6]-shogaol and [6]-gingerol exerted anti-invasive activity against human hepatocarcinoma cells.…”

In vitro antifungal activity and mechanism of essential oil from fennel (Foeniculum vulgare L.) on dermatophyte species

“…The essential oils obtained by steam distillation of the seeds show antioxidant (Ruberto et al, 2000), antithrombotic (Tognolini et al, 2007), antidiabetic (El-Soud et al, 2011) and acaricidal (Lee, 2004) activities. Fennel seed essential oil (FSEO) showed broad-spectrum antibacterial effects against pathogens such as Escherichia coli, Bacillus spp., Staphylococcus aureus, Micrococcus luteus, Pseudomonas spp., Listeria innocua CECT 910, Serratia marcescens CECT 854 and Pseudomonas fluorescens CECT 844 (Mohsenzadeh, 2007;Singh et al, 2006;Mahady et al, 2005;Kazemi et al, 2012). It also exhibited antifungal effects against pathogens such as Aspergillus spp., Fusarium spp., Candida spp., Sclerotinia sclerotiorum and Trichophyton mentagrophytes (Roby et al, 2013;Viuda-Martos et al, 2011;Inouye et al, 2006).…”

Achillea millefolium Aqueous Extract does not Impair Recognition Memory in Mice

“…For example, it is reported that a methanol extract of Achillea is active against helicobacter pylori [20]. A lipophilic fraction from A. millefolium reduced pain responses in an acetic acid-induced writhing test.…”