Achillea millefolium Aqueous Extract does not Impair Recognition Memory in Mice

Ayoobi, Fatemeh; Roohbakhsh, Ali; Allahtavakoli, Mohammad; Vazirinejad, Reza; Rajabi, Soodeh; Shamsizadeh, Ali

doi:10.4314/tjpr.v12i2.12

Cited by 3 publications

(2 citation statements)

References 18 publications

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“…(3) Group 3 (DAM): diabetic rats treated with intraperitoneal hydro-alcoholic extract of Achillea millefolium at a concentration of 250 mg/kg for 21 days (Ayoobi et al, 2013). (4) Group 4 (DG): Diabetic rats treated with glibenclamide (5 mg/kg) for 21 days (Abtahi-Evari et al, 2017).…”

Section: Methodsmentioning

confidence: 99%

Protective effect of hydro-alcoholic extract ofachillea millefoliumon renal injury and biochemical factors in streptozotocin-induced diabetic rats

Karimi

Niazkar

Azar

et al. 2021

NFS

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Purpose Herbal medicine has been used for the management of complications of diabetes. The purpose of this paper is to examine the anti-diabetic effects of Achillea millefolium extract on diabetic rats. Design/methodology/approach To this aim, 32 male Wistar rats were randomly assigned into four groups in which each group comprised eight rats. The four experimental groups were as follows: control group, diabetic control (DC) group (STZ; 50 mg/kg), diabetic rats, receiving 250 mg/kg hydro-alcoholic extract of the A. millefolium (DAM) and diabetic rats, receiving 5 mg/kg glibenclamide (DG). After 21 days of the treatment course, tissues of the kidney and blood samples were collected for histopathological, biochemical and molecular analysis. Findings The concentration of malondialdehyde (MDA) and glucose serum were markedly reduced in the DC group while significantly increased in DG and DAM groups (1.11 ± 0.57 to 19.4 ± 3.5 and 17.8 ± 1.2 p = 0.002 and 325 ± 0.18 to 223 ± 0.11 and 211 ± 0.32 p = 0.02, respectively). Also, the activities of glutathione peroxidase (GPx) and superoxide dismutase (SOD) were markedly reduced in the DC group while significantly increased in DAM and DG groups (9.1 ± 2.21 to 18.7 ± 3.81 and 14.9 ± 3.1 p = 0.03 and p = 0.02, respectively). The concentrations of creatinine, blood urea nitrogen (BUN) and urea were substantially decreased in DAM and DG groups as compared with the DC group (0.49 ± 0.02 to 0.27 ± 0.01 and 0.25 ± 0.01 p = 0.01, 15.6 ± 2.1 to 7.2 ± 0.68 and 8.6 ± 1.2 p = 0.02 and 114 ± 9.4 to 59.8 ± 5.2 and 64 ± 5.2 p = 0.03, respectively). Also, Bcl-2-associated X protein (BAX) expression was significantly decreased in DAM and DG groups as compared with the DC group (1.3 ± 0.32 to 0.91 ± 0.03 and 0.93 ± 0.02 p = <0.01) and Bcl-2 expression were significantly increased in DAM and DG groups as compared with the DC group (0.42 ± 0.05 to 0.88 ± 0.07 and 0.85 ± 0.06 p = 0.01). Originality/value Diabetes led to degenerative damages in the kidney of rats and increased the mRNA level of Bax, while treatment with A. millefolium could protect the kidney tissue against diabetes complications and increased the mRNA expression of Bcl-2. This study indicated that A. millefolium extracts not only improves renal function as a result of anti-oxidant activity but also modulates some biochemical factors in diabetic rats.

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Section: Methodsmentioning

confidence: 99%

Protective effect of hydro-alcoholic extract ofachillea millefoliumon renal injury and biochemical factors in streptozotocin-induced diabetic rats

Karimi

Niazkar

Azar

et al. 2021

NFS

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“…After that, the aqueous extract was separated by a rotary machine (20). The selective dose in this study was 200 mg/kg, based on previous studies (19,21). Due to the fact that the most of rat weighed around 250 g, we converted the mentioned dose per rat (3.5, 7 mg/rat) (19,21).…”

Section: Preparation Of Extractsmentioning

confidence: 99%

Effect of Achillea millefolium aqueous extract on memory deficit and anxiety caused by stroke in ovariectomized rats

Jahromi

Imani

Nasehi

et al. 2019

J Herbmed Pharmacol

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Introduction: Some studies indicated that the decrease of estrogen level in menopausal woman results in increasing the risk of stroke. Although estrogen is a neuroprotective factor, high consumption of this compound may develop breast cancer and endometriosis. The present study investigated the effect of Achilles millefolium extract, containing estrogenlike compounds, on memory impairment and anxiogenic-like behaviors caused by cerebral ischemia in ovariectomized rats. Methods: Permanent middle cerebral artery ligation was performed, as a model for studying postmenopausal condition, in 48 female Wistar rats weighing 200-250 g. The aqueous extract of A. millefolium was prepared and gavaged for 4 weeks after inducing cerebral ischemia. Memory and anxiety-like behavior assessments were evaluated by step-through and elevated plus maze apparatus, respectively. Result: According to the results, cerebral ischemia in ovariectomized rats induced amnesia and anxiogenic-like behaviors which were restored by 7 mg/kg of A. millefolium aqueous extract. Furthermore, inactivation of estrogen receptors (ERs) by tamoxifen (100 µg/kg, intraperitoneally) blocked the restoration effect of A. millefolium on behaviors induced by cerebral ischemia. Conclusion: It could be concluded that, oral administration of A. millefolium extract is able to restore memory impairment and anxiogenic-like behaviors induced by ischemia via ERs in ovariectomized rat.

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