In vitro study on the effect of larval excretory/secretory products and crude extracts from Anisakis simplex on blood coagulation

Perteguer, María Jesús; Raposo, Rafaela; Cuéllar, C.

doi:10.1016/0020-7519(95)00111-5

Cited by 31 publications

(27 citation statements)

References 5 publications

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“…Only healthy larvae randomly collected from several infrapopulations were carefully selected under a stereomicroscope and placed in a defined, complex specific culture medium follow Perteguer et al (1996). After 7 d culture, the larvae were removed and fixed in hot 70% ethanol.…”

Section: Methodsmentioning

confidence: 99%

Fitness of the marine parasitic nematode Anisakis simplex s.str. in temperate waters of the NE Atlantic

Abollo¹,

D’Amelio²,

Pascual³

2001

Dis. Aquat. Org.

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In temperate waters of the NE Atlantic, third-stage larvae of Anisakis simplex sensu stricto collected from 3 paratenic host species were identified by restriction fragment length polymorphisms. The condition of wild larval infrapopulations was assessed by examining morphometric and growth characteristics. The differentiation patterns and the excretory/secretory products of larvae grown in vitro were also examined. An extensive morphometric, growth and differentiation variability was found between parasite larvae collected from different paratenic host sources. Nematode infrapopulation larvae from the squid comprise those smaller individuals with the lowest values of survival rates and moult success. It may be then concluded that the fitness of A. simplex s. str. larvae is not the best possible in the squid, which impaired the competitiveness of the parasite and its chances of developing into an adult. This suggests that the microenvironments impaired by the paratenic host may provide larval infrapopulations with unique ecological factors probably influencing its recruitment to the final host populations. KEY WORDS: Anisakis simplex s. str. · Fitness · RecruitmentResale or republication not permitted without written consent of the publisher

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Section: Methodsmentioning

confidence: 99%

Fitness of the marine parasitic nematode Anisakis simplex s.str. in temperate waters of the NE Atlantic

Abollo¹,

D’Amelio²,

Pascual³

2001

Dis. Aquat. Org.

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“…In 1994, a 40-kDa enzyme that degrades chondroitin sulfate-A and hyaluronic acid (114) and a 30-kDa serine protease similar to that present in the bacterium Dichelobacter nodosus (193) were characterized. The invasive capacity of the larvae, together with the presence of anticoagulant substances in the ES products, explains the existence of the multiple, well-defined, erosive, and/or hemorrhagic lesions usually detected near the main lesion within the gastric mucosa of patients suffering from anisakidosis (198,213,217).…”

Section: Invasive Mechanismsmentioning

confidence: 99%

“…The metabolic products released by the larva are also im- (223,224), mutagenic properties by components of 10 kDa (55, 217), anticoagulant activity (213,217), and chemotaxis of eosinophils associated with thermolabile factors from the parasites (84, 259) that do not damage the surface of larvae but are partly responsible for the tissue damage induced in the host.…”

Section: Invasive Mechanismsmentioning

confidence: 99%

Anisakis simplex: from Obscure Infectious Worm to Inducer of Immune Hypersensitivity

2008

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SUMMARY Infection of humans with the nematode worm parasite Anisakis simplex was first described in the 1960s in association with the consumption of raw or undercooked fish. During the 1990s it was realized that even the ingestion of dead worms in food fish can cause severe hypersensitivity reactions, that these may be more prevalent than infection itself, and that this outcome could be associated with food preparations previously considered safe. Not only may allergic symptoms arise from infection by the parasites (“gastroallergic anisakiasis”), but true anaphylactic reactions can also occur following exposure to allergens from dead worms by food-borne, airborne, or skin contact routes. This review discusses A. simplex pathogenesis in humans, covering immune hypersensitivity reactions both in the context of a living infection and in terms of exposure to its allergens by other routes. Over the last 20 years, several studies have concentrated on A. simplex antigen characterization and innate as well as adaptive immune response to this parasite. Molecular characterization of Anisakis allergens and isolation of their encoding cDNAs is now an active field of research that should provide improved diagnostic tools in addition to tools with which to enhance our understanding of pathogenesis and controversial aspects of A. simplex allergy. We also discuss the potential relevance of parasite products such as allergens, proteinases, and proteinase inhibitors and the activation of basophils, eosinophils, and mast cells in the induction of A. simplex-related immune hypersensitivity states induced by exposure to the parasite, dead or alive.

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“…Anisakis simplex L3 crude extract (CE) was prepared according to Perteguer et al (1996). Brie¯y, larvae were harvested manually from the viscera,¯esh, and body cavity of naturally infected blue whiting (Micromesistius poutassou), washed thoroughly in water and stored at 4°C.…”

Section: Parasites and Antigenmentioning

confidence: 99%

Cellular immune responses in mice immunized with Anisakis simplex larval antigens

et al. 2001

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Cellular immune responses to Anisakis simplex L3 antigens were investigated in BALB/c mice injected subcutaneously with a homologous crude extract (CE). Popliteal lymph nodes (PLN) were found to be increased in size and weight after A. simplex CE footpad injection. The effects of A. simplex CE in vitro proliferation were assayed with non-fractionated PLN cells or nylon-wool purified T cells derived from pooled lymph node cells of mice subcutaneously injected with CE. Spleen cells from immunized animals (antigen alone, or larva alone, or antigen plus larva) were studied by flow cytometry. The immunization induced a high proportion of CD4 + and TCRalphabeta + T cells. The number of B cells (CD45 + and TCRalphabeta-) in pre-immunized and infected mice was lower than that observed in animals subjected to infection only. The number of CD4+ T cells increased in the infected and in the pre-immunized and infected mice. In the latter, a decrease of CD8a + T cells was noted. The greatest increase in CD8a+ and TCRalphabeta- T cells was found in mice that had been subjected to infection only. Histological analysis showed that the most prominent lesions were gastric and intestinal in animals infected orally with one larva.

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In vitro study on the effect of larval excretory/secretory products and crude extracts from Anisakis simplex on blood coagulation

Cited by 31 publications

References 5 publications

Fitness of the marine parasitic nematode Anisakis simplex s.str. in temperate waters of the NE Atlantic

Fitness of the marine parasitic nematode Anisakis simplex s.str. in temperate waters of the NE Atlantic

Anisakis simplex: from Obscure Infectious Worm to Inducer of Immune Hypersensitivity

Cellular immune responses in mice immunized with Anisakis simplex larval antigens

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