In Vitro Study on Anti-Hepatitis C Virus Activity of Spatholobus suberectus Dunn

Abstract

Hepatitis C virus (HCV) infects 200 million people worldwide, and 75% of HCV cases progress into chronic infections, which consequently cause cirrhosis and hepatocellular carcinoma. HCV infection is treated with currently considered standard drugs, including direct anti-viral agents (DAAs), alone or in combination with peginterferon-α plus ribavirin. However, sustained viral responses vary in different cohorts, and high costs limit the broad use of DAAs. In this study, the ethanol and water extracts of 12 herbs from Lingnan in China were examined in terms of their inhibitory effect on HCV replication. Among the examined extracts, Spatholobus suberectus ethanol extracts suppressed HCV replication. By comparison, Extracts from Fructus lycii, Radix astragali (root), Rubus chingii Hu (fruit), Flos chrysanthemi Indici (flower), Cassia obtusifolia (seed), Lonicera japonica Thunb (flower), Forsythia suspense Thunb (fruit), Poria cocos (sclerotia), Carthamus tinctorius L. (flower), Crataegus pinnatifida Bge. (fruit), and Leonurus japonicas Houtt. (leaf) extracts failed to show a similar activity. Active S. suberectus fractions containing tannins as the major component also inhibited the in vitro translation of HCV RNA. The combination treatments of single compounds, such as epigallocatechin gallate and epicatechin gallate, were not as potent as crude S. suberectus fractions; therefore, crude S. suberectus extract may be a potential alternative treatment against HCV either alone or in combination with other agents.

Comparative Genomics of Spatholobus suberectus and Insight Into Flavonoid Biosynthesis

“…Determination of total flavonoid content (TFC): The TFC was carried out according to Chen et al. (2016) .…”

“…Determination of total flavonoid content (TFC): The TFC was carried out according to Chen et al (2016). Dry samples (0.3 g) and sonicated in 25 ml of 50% ethanol at a ratio of 1:20 (w/v) for 1 h using a SB-800 DTD sonicator (Ningbo Xinzhi Biotechnology Co., Ltd, Ningbo, China; power: 100 W; frequency: 40 kHz).…”

Spatholobus suberectus extract suppresses proliferation and EMT, and promotes apoptosis in palmitic acid induced vascular endothelial cells by inhibiting LncRNA MALAT1 via VEGF signaling pathway

“…Spatholobus suberectus (SS) Dunn is dried vine widely used in traditional medicine because it possesses pharmacological activities that include improvement of blood circulation, antiplatelet, anti-inflammation, anti-bacterial, neuroprotection, and anti-cancer effects [6,17]. The plant is known to contain many secondary compounds and flavonoids are the major bioactive substances, including 3′,4′,7-trihydroxyglavone, formononetin, calycosin, prunetin, eriodictyol, butin, liquiritigenin, plathymenin, dihydroquercetin and dihydrokaempferol as well as sterols, lignans, anthraquinones and glycosides [7,8,18].…”

Spatholobus suberectus Ameliorates Diabetes-Induced Renal Damage by Suppressing Advanced Glycation End Products in db/db Mice

“…Component analysis of S . suberectus showed that the herb contains various types of phenolic compounds, including flavones, isoflavonoids, flavanones, flavanonols, and chalcones [ 16 ]. The herb has been shown to have diverse benefits including anti-inflammatory, antioxidant, and antirheumatic effects [ 17 ].…”