In vitro study of soil arsenic release by human gut microbiota and its intestinal absorption by Caco-2 cells

Yin, Naiyi; Cai, Xiaolin; Du, Hongwang; Zhang, Zhennan; Li, Zejiao; Chen, Xiaochen; Sun, Guoxin

doi:10.1016/j.chemosphere.2016.10.091

Cited by 27 publications

(15 citation statements)

References 44 publications

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“…To our knowledge, this was the first ever report of the bioaccessibility of Cu, Fe, Mn, and Zn in the market vegetables in the colon, which suggested that the previous in vitro studies without simulating the colon phase could have overestimated the bioaccessibility and bioavailability of Cu while underestimated those of Fe, Mn, and Zn. Under the anaerobic environment of the colon, human gut microbiota could induce reductive dissolution of Fe and Mn 22 , although the secondary Fe/Mn (hydr)oxides formation could inhibit their dissolution 18 , 28 . Besides, these trace metals present as organometallic salts in vegetables could be nutritional supplementation to the gut microbiota, resulting in their further release in the colon.…”

Section: Resultsmentioning

confidence: 99%

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Investigation of bioaccessibility of Cu, Fe, Mn, and Zn in market vegetables in the colon using PBET combined with SHIME

Yin

Cai

Chen

et al. 2017

Sci Rep

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The in vitro bioaccessibility of trace metals associated with oral ingestion of market vegetables (lettuce, pak choi, cole, and leaf lettuce) of Beijing, China was studied. The physiologically based extraction test (PBET) combined with the Simulator of Human Intestinal Microbial Ecosystem (SHIME) was applied to simulate stomach, small intestine, and colon of human. In the gastro-intestinal phases, the bioaccessibility of Cu, Fe, Mn, and Zn varied within 5.7–75.5%, 17.3–50.4%, 13.3–49.1%, and 19.9–63.7%, respectively. There was no significant difference in the metal bioaccessibility between the gastric and small intestinal phases, except for higher Cu bioaccessibility in the small intestine. Besides, the bioaccessibility of the four trace metals in the colon phase was first ever reported. A significant decline in Cu bioaccessibility (1.8–63.7%) and slight increases in the bioaccessibility of Fe (16.7–56.4%), Mn (21.2–71.6%), and Zn (15.7–69.7%) were revealed, which could mainly be attributed to the effect of colon microbiota. In addition, the estimated daily intakes (EDIs) of Cu, Fe, Mn, and Zn were worked out to be 0.7, 8.8, 2.7, and 4.5 μg kg−1 body weight d−1, based on which the potential influences of these trace metals in vegetables on the health of the local consumers was demonstrated.

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Section: Resultsmentioning

confidence: 99%

“… 12 and Yin et al . 22 . Briefly, the fresh vegetable sample (3 g) in triplicate was added to a polypropylene conical centrifuge tube (50 mL) with the digest (30 mL) at a solid/solution (s/s) ratio of 1:10 in the gastric and small intestinal phases.…”

Section: Methodsmentioning

confidence: 99%

Investigation of bioaccessibility of Cu, Fe, Mn, and Zn in market vegetables in the colon using PBET combined with SHIME

Yin

Cai

Chen

et al. 2017

Sci Rep

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“…The same authors using SHIME, analyzed the interactions between four soils with different As concentrations and fecal microorganisms, demonstrating that human GM can release soil-bound As. In humans subjected to soil As exposure, an precise risk assessment could be carried out by concurrently defining As transformation and intestinal absorption (46).…”

Section: Arsenicmentioning

confidence: 99%

Influence of toxic metal exposure on the gut microbiota (Review)

et al. 2021

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The gut microbiota (GM) is composed of >100 trillion different organisms, including bacteria, viruses, fungi, archaea and protists, coexisting in a complex system. The GM can be very sensitive to drugs, diet or even environmental pollutants. In the present review, recent data related to the interaction between the GM and heavy/toxic metals are discussed, focusing on the compounds most widely distributed in the environment or considered biopersistent. There are data to suggest that exposure to metals can alter the composition, diversity, homogeneity and structure of the GM. The specific modifications reported are not homogeneous, and a number of factors may explain this variability, including differences in metal compound, exposure modalities (e.g., food, water, in vitro), exposure time, qualitative and quantitative diversity of bacterial species in basal microbiota and analytical issues. As regards metal nanoparticles, some authors foresee the premises for a safe preventive and therapeutic use, while others have revealed harmful effects on both the gut microbiome and health. These findings, which would benefit from the application of modern approaches such as metagenomic sequencing and metabolomics, seem to indicate structural and functional analysis of gut microbiota as an early biomarker of detrimental effects from exposure to metals.

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“…Furthermore, As metabolism by human gut microbiota and intestinal absorption of these As metabolites should be concurrent processes. Intestinal absorption of different As standard species or metabolites is examined using in vitro studies with the Caco-2 human cell line of human colon carcinoma (Hinrichsen et al, 2015;Yin et al, 2017). In short, methylated trivalent arsenicals display a higher transport across epithelial cells in comparison with inorganic arsenic and the methylated pentavalent species.…”

Section: Introductionmentioning

confidence: 99%

Interindividual variability of soil arsenic metabolism by human gut microbiota using SHIME model

Yin

Wang

et al. 2017

Chemosphere

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Arsenic (As) metabolism by human gut microbiota has been evidenced with in vitro experiments from contaminated soils. In this study, the variability in the metabolic potency toward As-contaminated soils and gut microbial diversity were investigated between healthy individuals (Adult versus Child). Arsenic bioaccessibility in the colon phase increased by 1.4-6.8 and 1.2-8.7 folds for adult and child, respectively. We found a high degree of As methylation for the colon digests of the adult (mean 2 μg methylarsenicals/hr/g biomass), 3-folds higher than that of the child. Besides, arsenite [As(III)] concentration (1.5-391.3 μg/L) for the child was 2-18 times for the adult. 16S rRNA gene sequencing revealed that human gut microbiota from 20 various genera potentially had resistance genes to reduce and methylate As under conservative statistics. Our results indicated that As metabolism by gut microbiota from adult and child was significantly different. The adult gut microbiota had a great ability of As methylation; the child gut microbiota exhibited high As(III) level, which could encounter high health risk. The identity and activity of arsenic-metabolizing bacteria isolated from human gut and its homologous role in As metabolism need be further explored. This study provides a better understanding of health risk assessment to adults and children upon soil As exposures.

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In vitro study of soil arsenic release by human gut microbiota and its intestinal absorption by Caco-2 cells

Cited by 27 publications

References 44 publications

Investigation of bioaccessibility of Cu, Fe, Mn, and Zn in market vegetables in the colon using PBET combined with SHIME

Investigation of bioaccessibility of Cu, Fe, Mn, and Zn in market vegetables in the colon using PBET combined with SHIME

Influence of toxic metal exposure on the gut microbiota (Review)

Interindividual variability of soil arsenic metabolism by human gut microbiota using SHIME model

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