In vitro study of anti-ER positive breast cancer effect and mechanism of 1,2,3,4-6-pentyl-O-galloyl-beta-d-glucose (PGG)

Qiu, Xiang; Tang, Juan; Luo, Qin; Xue, Jinfeng; Tao, Yexing; Jiang, Hui; Tian, Jing; Fan, Caiwen

doi:10.1016/j.biopha.2018.12.062

Cited by 13 publications

(10 citation statements)

References 45 publications

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“…[ 1 ] Estrogen receptor (ER) positive breast cancer represents up to 80% of all breast cancers. [ 2 ] Globally, increasing numbers of diagnosed cases have triggered a search for effective treatment for breast cancer. There are several ways for the management of breast cancer for example, surgery, chemotherapy, etc.…”

Section: Introductionmentioning

confidence: 99%

Gallic acid potentiates the apoptotic effect of paclitaxel and carboplatin via overexpression of Bax and P53 on the MCF‐7 human breast cancer cell line

Aborehab

Elnagar

Waly

2020

J Biochem & Molecular Tox

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Despite advances in treatment, breast cancer remains the widest spread disease among females with a high mortality rate. We investigated the potential effects of gallic acid (GA) as supportive therapy in the management of breast cancer. Anti‐cancer activity with GA alone or in combination with paclitaxel and/or carboplatin was assessed by MTT assay and flow cytometry using annexin V/propidium iodide. The mechanism underlying the antiproliferative effects was investigated by measuring the expression of the pro‐apoptotic marker (Bax), CASP‐3, anti‐apoptotic (Bcl‐2), and, tumor suppressor (p53) by real‐time polymerase chain reaction (RT‐PCR) and western blot analysis. Cell cycle analysis was performed for the MCF‐7 breast cancer cell line. GA, paclitaxel, and carboplatin alone or in combination arrested cell cycle progression at the G2/M phase and induced Pre‐G1 apoptosis. RT‐PCR showed that the triplet combination significantly raised P53, Bax, and CASP‐3 mRNA expression (20.1 ± 1.41, 16.6 ± 0.43, and 20.04 ± 1.61, respectively) in MCF‐7 cells when compared to single or combined treatment (p < .0001) while anti‐apoptotic Bcl‐2 mRNA levels were decreased in all treated groups compared to untreated cells. Western blot data of tested apoptotic factors were consistent with RT‐PCR results. For the first time, we show that a minimum non‐toxic concentration of GA increased the efficacy of paclitaxel‐ and carboplatin‐induced MCF‐7 apoptotic cell death.

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Section: Introductionmentioning

confidence: 99%

Gallic acid potentiates the apoptotic effect of paclitaxel and carboplatin via overexpression of Bax and P53 on the MCF‐7 human breast cancer cell line

Aborehab

Elnagar

Waly

2020

J Biochem & Molecular Tox

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“…Tannic acid can also inhibit fatty acid synthetase and bind to lipids to inhibit the proliferation of cancer cells (31). that low concentrations of PGG blocked the cycle of ER + breast cancer T-47D and BT-474 cells in S phase, whereas high concentrations of PGG blocked the cycle of cells in G1 phase (14). Moreover, PGG was found to downregulate the expression of HURP and BCL-2, and increase the expression of BAX to induce apoptosis as an anti-ER breast cancer (14).…”

Section: Discussionmentioning

confidence: 99%

“…It has shown strong biological and pharmacological activities in antiviral ( 9 ), anti-inflammatory ( 10 ), anti-microbial ( 11 ), and anti-diabetic ( 12 ). Previous studies have shown that β-PGG exhibits inhibitory effects on colon cancer ( 13 ), breast cancer ( 14 ), prostate cancer ( 15 ), and pancreatic cancer ( 16 ). However, there is a lack of reports on the anti-hepatocellular carcinoma effects of β-PGG and the possible mechanisms.…”

Section: Popular Scientific Summarymentioning

confidence: 99%

In vitro anti-hepatocellular carcinogenesis of 1,2,3,4,6-Penta-O-galloyl-β-D-glucose

Jiang

et al. 2023

Food & Nutrition Research

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Background: 1,2,3,4,6-Penta-O-galloyl-β-D-glucose (β-PGG) is a polyphenol ellagic compound with a variety of pharmacological effects and has an inhibitory effect on lots of cancers. Objective: To explore the antitumor effects and mechanism of 1,2,3,4,6-Penta-O-galloyl-β-D-glucose on human hepatocellular carcinoma HepG2 cells. Design: A network pharmacology method was first used to predict the possible inhibition of hepatocellular carcinoma growth by 1,2,3,4,6-Penta-O-galloyl-β-D-glucose (β-PGG) through the p53 signaling pathway. Next, the Cell Counting Kit (CCK-8) assay was performed to evaluate changes in the survival rate of human hepatocellular carcinoma HepG2 cells treated with different concentrations of the drug; flow cytometry was used to detect changes in cell cycle, apoptosis, mitochondrial membrane potential (MMP) and intracellular Ca2+ concentration; real-time fluorescence quantification and immunoblotting showed that the expression of P53 genes and proteins associated with the p53 signaling pathway was significantly increased by β-PGG treatment. Reasult: It was found that β-PGG significantly inhibited survival of HepG2 cells, promoted apoptosis, decreased MMP and intracellular Ca2+ concentration, upregulated P53 gene and protein expression, increased CASP3 expression, and induced apoptosis in HepG2 cells. Conclusion: This study has shown that network pharmacology can accurately predict the target of β-PGG’s anti-hepatocellular carcinoma action. Moreover, it was evident that β-PGG can induce apoptosis in HepG2 cells by activating the p53 signaling pathway to achieve its anti-hepatocellular carcinoma effect in vitro.

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“…both ER+ and ER-breast cancer cell lines. Additionally, PGG induced G0/G1 and S phase cell cycle arrest and apoptosis in T-47D and BT-474 cells by inhibiting cyclin D1and by affecting specific apoptosis-related proteins such as Bax and Bcl-2[31]. PGG was also capable of inhibiting triple-negative breast xenograft growth and metastasis via Expression of apoptosis-related genes in MCF-7 cells after MPSE treatment as determined by RT-PCR.…”

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confidence: 94%

Pentagalloyl Glucose‐ and Ethyl Gallate‐Rich Extract from Maprang Seeds Induce Apoptosis in MCF‐7 Breast Cancer Cells through Mitochondria‐Mediated Pathway

Kantapan

Paksee²,

Chawapun

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Bouea macrophylla Griffith, locally known as maprang, has important economic value as a Thai fruit tree. The maprang seed extract (MPSE) has been shown to exhibit antibacterial and anticancer activities. However, the bioactive constituents in MPSE and the molecular mechanisms underlying these anticancer activities remain poorly understood. This study aims to identify the active compounds in MPSE and to investigate the mechanisms involved in MPSE-induced apoptosis in MCF-7 treated cancer cells. The cytotoxic effect was determined by MTT assay. The apoptosis induction of MPSE was evaluated in terms of ROS production, mitochondrial membrane potential depolarization, and apoptosis-related gene expression. The compounds identified by HPLC and LC/MS analysis were pentagalloyl glucose, ethyl gallate, and gallic acid. MPSE treatment decreased cell proliferation in MCF-7 cells, and MPSE was postulated to induce G2/M phase cell cycle arrest. MPSE was found to promote intracellular ROS production in MCF-7 treated cells and to also influence the depolarization of mitochondrial membrane potential. In addition, MPSE treatment can lead to increase in the Bax/Bcl-2 gene expression ratio, suggesting that MPSE-induced apoptosis is mitochondria-dependent pathway. Our results suggest that natural products obtained from maprang seeds have the potential to target the apoptosis pathway in breast cancer treatments.

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In vitro study of anti-ER positive breast cancer effect and mechanism of 1,2,3,4-6-pentyl-O-galloyl-beta-d-glucose (PGG)

Cited by 13 publications

References 45 publications

Gallic acid potentiates the apoptotic effect of paclitaxel and carboplatin via overexpression of Bax and P53 on the MCF‐7 human breast cancer cell line

Gallic acid potentiates the apoptotic effect of paclitaxel and carboplatin via overexpression of Bax and P53 on the MCF‐7 human breast cancer cell line

In vitro anti-hepatocellular carcinogenesis of 1,2,3,4,6-Penta-O-galloyl-β-D-glucose

Pentagalloyl Glucose‐ and Ethyl Gallate‐Rich Extract from Maprang Seeds Induce Apoptosis in MCF‐7 Breast Cancer Cells through Mitochondria‐Mediated Pathway

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