In vitro anti-hepatocellular carcinogenesis of 1,2,3,4,6-Penta-O-galloyl-β-D-glucose

Abstract

Background: 1,2,3,4,6-Penta-O-galloyl-β-D-glucose (β-PGG) is a polyphenol ellagic compound with a variety of pharmacological effects and has an inhibitory effect on lots of cancers.
 Objective: To explore the antitumor effects and mechanism of 1,2,3,4,6-Penta-O-galloyl-β-D-glucose on human hepatocellular carcinoma HepG2 cells.
 Design: A network pharmacology method was first used to predict the possible inhibition of hepatocellular carcinoma growth by 1,2,3,4,6-Penta-O-galloyl-β-D-glucose (β-PGG) through the p53 signaling pathway. Next, the Cell Counting Kit (CCK-8) assay was performed to evaluate changes in the survival rate of human hepatocellular carcinoma HepG2 cells treated with different concentrations of the drug; flow cytometry was used to detect changes in cell cycle, apoptosis, mitochondrial membrane potential (MMP) and intracellular Ca2+ concentration; real-time fluorescence quantification and immunoblotting showed that the expression of P53 genes and proteins associated with the p53 signaling pathway was significantly increased by β-PGG treatment.
 Reasult: It was found that β-PGG significantly inhibited survival of HepG2 cells, promoted apoptosis, decreased MMP and intracellular Ca2+ concentration, upregulated P53 gene and protein expression, increased CASP3 expression, and induced apoptosis in HepG2 cells.
 Conclusion: This study has shown that network pharmacology can accurately predict the target of β-PGG’s anti-hepatocellular carcinoma action. Moreover, it was evident that β-PGG can induce apoptosis in HepG2 cells by activating the p53 signaling pathway to achieve its anti-hepatocellular carcinoma effect in vitro.

Chemical Fingerprint Analysis and Content Determination of Horned Gallnut and Bellied Gallnut in Galla Chinensis

“…Peaks 8 and 2 could not be identified because of experimental limitations, which also was the shortcoming of this experiment, and subsequent studies will be combined with mass spectrometry to help identify more compounds in HG and BG. PGG has shown strong biological and pharmacological activities in antiviral, anticancer, anti-inflammatory, antimicrobial, and antidiabetic [ 27 ]. There are diverse scientific reports on the biological and pharmacological activities of GA, focused on antioxidant, antimicrobial, anti-inflammatory, anticancer, cardioprotective, gastroprotective, and neuroprotective effects [ 28 ].…”

Amino Acid Derivatives of Ginsenoside AD-2 Induce HepG2 Cell Apoptosis by Affecting the Cytoskeleton

“…A duration of 24 h after transfection, the supernatant and washing liquid of each group of cells were collected, the cells were digested with 0.25% trypsin (without EDTA), centrifuged together with the above supernatant and culture medium, and were washed twice with PBS. Preprepared 1× Annexin V Binding solution was used to prepare a cell suspension with a final concentration of 1 × 106 cells/mL [46,47]. The apoptosis rate was measured by flow cytometry (FACSVerse, Becton, Dickinson and Company, Franklin Lakes, NJ, USA).…”