In Vitro Studies on the Role of Biotin in the Metabolism of Rumen Microorganisms

Milligan, L. P.; Asplund, J. M.; Robblee, A. R.

doi:10.4141/cjas67-008

Cited by 12 publications

(13 citation statements)

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“…Average milk yields increased quadratically (Po0.05, Table 2) with the increasing level of biotin supplementation. Supplemental biotin improved fiber digestion in vitro (Bentley, 1954), and increased production of propionic acid (Milligan et al, 1967). All the major cellulolytic bacteria in the rumen require biotin for growth (Baldwin and Allison, 1983).…”

Section: Feed Intake and Milk Yieldmentioning

confidence: 98%

Effects of dietary biotin supplementation on performance and hoof quality of Chinese Holstein dairy cows

Chen¹,

Liu²

2012

Livestock Science

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Section: Feed Intake and Milk Yieldmentioning

confidence: 98%

Effects of dietary biotin supplementation on performance and hoof quality of Chinese Holstein dairy cows

Chen¹,

Liu²

2012

Livestock Science

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“…Diets containing more than 50% of DM as grain decreased ruminal synthesis of biotin in vitro compared with diets containing more forage (Abel et al, 2001). Bentley et al (1954) and Milligan et al (1967) showed that biotin supplementation increased fiber digestion in vitro, and that ruminal bacteria also require biotin for propionate production. Propionate production by mixed ruminal bacteria was reduced in vitro when biotin was not included in the culture media (Bentley et al, 1954;Milligan et al, 1967).…”

Section: Introductionmentioning

confidence: 97%

Effect of feeding biotin on milk production and hoof health in lactating dairy cows: A quantitative assessment

Lean

Rabiee

2011

Journal of Dairy Science

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Objectives of this study were to critically review randomized controlled trials, evaluate the effectiveness of supplementation with biotin on milk yield and composition and hoof health in lactating dairy cows, explore sources of heterogeneity among studies, and evaluate publication bias. Quantitative assessments can increase the statistical power with which we study the effect of treatments, such as biotin, on outcomes. A total of 9 papers, with 6 production and 3 hoof health studies, met the eligibility criteria for meta-analysis. Eight studies evaluated various hoof lesions in biotin-supplemented cows that did not meet the inclusion criteria. Eleven comparisons were made of milk production responses to biotin treatment. Data extracted included the number of cows in control and treatment groups, measures of variance of responses (standard error or standard deviation) and P-values. Other data obtained included the duration of treatment before and after calving, parity, breed of cow, type and dose of biotin, delivery method of supplementation, and types of diets. Biotin increased milk production by 1.29 kg per head per day (95% confidence interval=0.35 to 2.18 kg) with no evidence of heterogeneity (I(2)=0.0%). Treatment did not affect milk fat or protein percentages, and a trend to increase fat and protein yields was observed. Milk production and composition results were not influenced by duration of treatment before calving, parity, or diet type. Assessment of biotin supplementation on hoof health indicated that more studies had improved rather than negative or neutral outcomes. The effect of biotin treatment on milk production was relatively large and the effects on fat and protein yields, although not significant, were consistent in direction and magnitude with the milk response. The hoof health responses to biotin should encourage further studies to more effectively define the nature of these responses using consistent criteria for examination of hoof conditions and lameness.

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“…Biotin-dependent transcarboxylations are particularly involved in the succinate, or randomisation, pathway for propionate production associated with the activity of many ruminal cellulolytic species. When starch or soluble carbohydrates serve as the dominant substrates, there is a shift to the direct formation of propionate via the biotin-independent acrylate pathway (Milligan et al 1967;Scheifinger & Wolin, 1973).…”

mentioning

confidence: 99%

Effects of defaunation on fermentation characteristics and biotin balance in an artificial rumen-simulation system (RUSITEC) receiving diets with different amounts and types of cereal

Abel

Schröder

Lebzien³

et al. 2006

Br J Nutr

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Biotin is required by rumen microbes for efficient fermentation. To evaluate the role of protozoa in ruminal biotin metabolism, five diets composed of grass hay or of grass hay/cereal grain mixtures were supplied to faunated or defaunated RUSITEC fermenters. In the mixed diets, hay was replaced to 33:67 or 67:33 w/w on an air-dried basis by either wheat or maize grain in order to simulate different cellulolytic and amylolytic fermentation conditions. Defaunation increased SCFA production, whereas NH 4 concentration and the release of CH 4 were reduced. Biotin input declined when cereal grain was used to replace the hay. With the exception of the high-wheat treatment, defaunated fermenters yielded higher biotin outputs than faunated fermenters. The biotin balance, calculated as the difference between the total biotin output (biotin in the solid residue contained in the nylon bags after fermentation plus the biotin in the effluent) and the biotin input with the feed, was negative for all the dietary treatments apart from fermenters supplied with the high-maize diet. It was less negative or, in the case of the high-maize diets, more positive for defaunated compared with faunated fermenters. It was concluded that, under normal faunated conditions, protozoa directly utilise or indirectly affect the bacterial synthesis and/or utilisation of biotin. With diets of a high fermentation potential, as realised with the high-wheat diet, protozoa prevent the development of a bacterial population that would utilise high or synthesise low amounts of biotin.

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In Vitro Studies on the Role of Biotin in the Metabolism of Rumen Microorganisms

Abstract: For personal use only.

Cited by 12 publications

References 0 publications

Effects of dietary biotin supplementation on performance and hoof quality of Chinese Holstein dairy cows

Effects of dietary biotin supplementation on performance and hoof quality of Chinese Holstein dairy cows

Effect of feeding biotin on milk production and hoof health in lactating dairy cows: A quantitative assessment

Effects of defaunation on fermentation characteristics and biotin balance in an artificial rumen-simulation system (RUSITEC) receiving diets with different amounts and types of cereal

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