In vitro Studies on the Trypanocidal activities of various Phytochemical fractions obtained from Garcinia kola seed

Johnson, Titilayo Omolara; Ijeoma, KO; Ekanem, Ekpenyong E.; Nelson, Elias; Mohammed, B. J.

doi:10.4314/jmt.v13i2.70717

Cited by 6 publications

(11 citation statements)

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“…Initially, preliminary antitrypanosomal activity screening was conducted by an in vitro method, which is relatively simple, cheap and reliable. As reported previously [ 17 , 19 , 24 ], under this in vitro system, trypanosomes could survive for up to 4 h or more in the absence of drugs. Parasites motility constitutes a relatively reliable indicator of viability among most zooflagellate parasites and cessation or drop in motility of the parasites in test substances treated blood compared to that of parasite-loaded control blood without test substances was taken as a measure of trypanocidal activity [ 17 , 20 ].…”

Section: Resultssupporting

confidence: 79%

Antitrypanosomal activity of aloin and its derivatives against Trypanosoma congolensefield isolate

et al. 2014

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BackgroundThere is an urgent need for the development of new, cheap, safe and highly effective drugs against African trypanosomiasis that affects both man and livestock in sub-Saharan Africa including Ethiopia. In the present study the exudate of Aloe gilbertii, an endemic Aloe species of Ethiopia, aloin, aloe-emodin and rhein were tested for their in vitro and in vivo antitrypanosomal activities against Trypanosoma congolense field isolate. Aloin was prepared from the leaf exudate of A. gilbertii by acid catalyzed hydrolysis. Aloe-emodin was obtained by oxidative hydrolysis of aloin, while rhein was subsequently derived from aloe-emodin by oxidation. In vitro trypanocidal activity tests were conducted on parasites obtained from infected mice, while mice infected with T. congolense were used to evaluate in vivo antitrypanosomal activity of the test substances.ResultsResults of the study showed that all the test substances arrested parasites motility at effective concentration of 4.0 mg/ml within an incubation period ranging from 15 to 40 min. Moreover, the same concentration of the test substances caused loss of infectivity of the parasites to mice during 30 days observation period. Among the tested substances, rhein showed superior activity with minimum inhibitory concentration (MIC) of 0.4 mg/ml. No adverse reactions were observed when the test substances were administered at a dose of 2000 mg/kg. Rhein at doses of 200 and 400 mg/kg, and the exudate, aloin and aloe-emodin at a dose of 400 mg/kg reduced the level of parasitaemia significantly (P < 0.05) and improved anaemia.ConclusionThe results obtained in this investigation indicate that aloin and its derivatives particularly rhein have the potential to be used as a scaffold for the development of safe and cost effective antitrypanosomal drugs that can be useful in the continuing fight against African trypanosomiasis.

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Section: Resultssupporting

confidence: 79%

Antitrypanosomal activity of aloin and its derivatives against Trypanosoma congolensefield isolate

et al. 2014

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“…Recent efforts on ethnopharmacology has revealed several medicinal plants as potential trypanocides [25,26,27], and natural products such as alkaloids, terpenes, quinones, and polyphenols have been shown to exhibit trypanocidal activities [28]. The ethanolic crude extract of G. kola seed exhibited in vivo trypanocidal activity in line with the in vitro work earlier reported [19]. The trypanocidal activity of the extract could be linked to the presence of bioactive compounds, some of which were detected in this study.…”

Section: Discussionsupporting

confidence: 87%

“…The study showed that crude ethanolic extract of G. kola seeds and four phytochemical fractions obtained from it (alkaloid, flavonoid, saponin and cardiac glycoside fractions) exhibit varying degrees of trypanocidal activities in vitro. The alkaloid fraction showed the highest trypanocidal activity followed by the saponin fraction, the crude extract, the cardiac glycoside and the flavonoid fractions respectively [19]. In this study, the in vivo trypanocidal activity of the crude ethanolic extract and the alkaloid, flavonoid, saponin and cardiac glycoside fractions of G. kola seeds was determined.…”

Section: Introductionmentioning

confidence: 88%

“…Cardiac glycoside fraction was extracted with 5% KOH in n-butanol, saponin fraction with 10% HCl in n-butanol while alkaloid fraction was extracted in chloroform. Flavonoids were obtained from the remaining aqueous fraction [19,20]. The samples were appropriately dissolved in distilled water to obtain dosages corresponding to 50 and 100mg/kg rat weight.…”

Section: Extraction and Fractionation Of G Kola Seedsmentioning

confidence: 99%

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In vivo Trypanocidal Activity of Ethanolic Crude Extract and Phytochemical Fractions of Garcinia kola Seeds

Johnson

2014

ARRB

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Aim: To determine the trypanocidal activity of the crude ethanolic extract and phytochemical fractions of Garcinia kola seeds in Trypanosoma brucei brucei infected rats. Study Design: Trypanosoma brucei brucei infected rats were treated with the ethanolic crude extract, alkaloid, flavonoid, saponin and cardiac glycoside fractions of Garcinia kola seeds. Parasitaemia and Packed cell volume (PCV) were measured.

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“…The phytochemical analysis of the DCM and MeOH crude extracts of Albizia schimperiana revealed a range of compounds that could be responsible for the trypanosuppresive activity. Most plants with antitrypanosomal properties have been found to contain metabolites such as alkaloids [25][26][27], flavonoids [26,28] and saponins [29].…”

Section: Discussionmentioning

confidence: 99%

Invivo Anti-Trypanosomal Activity of the Leaf Extracts of Albizia Schimperiana (Fabaceae) Against Trypanosoma Congolense Infection in Mice

Shibeshi¹

2015

Clin Exp Pharmacol

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Objectives: The objective of this study was to evaluate effect of dichloromethane (DCM) and methanol (MeOH) extracts of the leaf of Albizia schimperiana against Trypanosoma congolense using in vivo mice models. Methods: The leaf of the plant was extracted by maceration technique using DCM and absolute MeOH to obtain the corresponding crude extracts. The extracts were screened for secondary metabolites and anti-trypanosomal activity of the crude extracts was evaluated at doses of 50, 100, 200 and 400 mg/kg in Swiss albino mice infected with T. congolense isolated from natural infection of cattle. The animals were monitored for test parameters including parasitemia, packed cell volume, rectal temperatures, body weight and survival. Results: The acute toxicity test showed that both solvent extracts were safe at doses of up to 2 g/kg. The methanol extract at 100, 200 and 400 mg/kg showed a statistically significant (p<0.05) trypanosuppresive effect, but was unable to completely clear trypanosomes. Significantly (p<0.05) higher packed cell volume (PCV), weight and survival time were observed in groups treated with higher doses of methanol extract, however, the DCM extract treated mice has not showed statistically significant (p>0.05) reduction in parasitemia except 400 mg/kg dose. Conclusion: It can conclude that MeOH extract has promising activity against T. congolense in mice by reducing the levels of parasitemia and the activities may be due to presence of alkaloid, flavonoid and saponins which are responsible for anti-trypanosomal activity.

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In vitro Studies on the Trypanocidal activities of various Phytochemical fractions obtained from Garcinia kola seed

Cited by 6 publications

References 0 publications

Antitrypanosomal activity of aloin and its derivatives against Trypanosoma congolensefield isolate

Antitrypanosomal activity of aloin and its derivatives against Trypanosoma congolensefield isolate

In vivo Trypanocidal Activity of Ethanolic Crude Extract and Phytochemical Fractions of Garcinia kola Seeds

Invivo Anti-Trypanosomal Activity of the Leaf Extracts of Albizia Schimperiana (Fabaceae) Against Trypanosoma Congolense Infection in Mice

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