In vitro studies on mesenchymal stem cells of a dry birch tree bark extract

Dehelean, Cristina; Tatu, Calin A.; Simona, Cîntă-Pînzaru; Peev, Camelia; Şoica, Codruţa; Tanasie, Gabriela; Anghel, S.

doi:10.1016/j.toxlet.2006.07.133

Cited by 4 publications

(4 citation statements)

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“…The separation chromatogram of the BC1 extract is also attached in the Figure 9 . As expected, two major compounds, betulin (58.8%) and lupeol (41%) were dominant in the BC1 extract, as indicated by the mass spectra, whereas the other two extracts, BC2 and B(MeOH) respectively, exhibited less triterpene content [ 25 ] (22.8% and 23.7% betulin, respectively), in agreement with the predicted vibrational data. The NIST library of spectra was used for reference.…”

Section: Resultssupporting

confidence: 84%

“…It was selected after comparation with another two types of extractions, using a Soxhlet (Gerhardt) continuos extraction: BC2 sample resulted from 4.93 g birch bark and 250 ml cloroform/diclormetan/metanol 1:1:1 solvents mixture for 2 hours, continuous extraction and B(MeOH) sample from 4.90 g dried sample with 250 ml methanol using a ultrasonication bath (Falc). According to the literature [ 1 , 9 , 10 , 20 , 26 ] using a mixture of solvents could result in an increased extraction products amount but we proved that the continous methanolic extract assure a good extraction yield for triterpene content. [ 8 ] In order to establish the end of extraction, the depletion of vegetal material was used.…”

Section: Methodsmentioning

confidence: 77%

“…The vegetal tissues contain complex mixtures of substances known to possess synergistic activities. [ 19 , 20 ] Structure-activity relationship studies [ 7 , 21 ] revealed that C-28 carboxylic acid of betulinic acid was very important for its cytotoxicity. A recent study for the simultaneous extraction and determination of betulin and betulinic acid in white birch bark using RP-HPLC has been reported.…”

Section: Resultsmentioning

confidence: 99%

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Evaluation and differentiation of the Betulaceae birch bark species and their bioactive triterpene content using analytical FT-vibrational spectroscopy and GC-MS

Pinzaru

Dehelean

Şoica

et al. 2012

Chemistry Central Journal

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BackgroundAiming to obtain the highest triterpene content in the extraction products, nine bark samples from the forest abundant flora of Apuseni Mountains, Romania were Raman spectroscopically evaluated. Three different natural extracts from Betula pendula Roth birch bark have been obtained and characterized using Fourier transform vibrational spectra.ResultsThis study shows that principal components of the birch tree extract can be rapidly recognized and differentiated based on their vibrational fingerprint band shape and intensity. The vibrational spectroscopy results are supported by the GC-MS data. Based on IR and Raman analysis, one can conclude that all the extracts, independent on the solvent(s) used, revealed dominant betulin species, followed by lupeol.ConclusionsSince Raman measurements could also be performed on fresh plant material, we demonstrated the possibility to apply the present results for the prediction of the highest triterpene content in bark species, for the selection of harvesting time or individual genotypes directly in the field, with appropriate portable Raman equipment.

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Section: Resultssupporting

confidence: 84%

Section: Methodsmentioning

confidence: 77%

Section: Resultsmentioning

confidence: 99%

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Evaluation and differentiation of the Betulaceae birch bark species and their bioactive triterpene content using analytical FT-vibrational spectroscopy and GC-MS

Pinzaru

Dehelean

Şoica

et al. 2012

Chemistry Central Journal

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“…According to the literature data [ 68 , 69 , 70 , 71 ], the use of a mixture of solvents can lead to an increase in the yield of extractive substances from birch bark. It has been shown that prolonged extraction with methanol provides the best yield of triterpenes [ 72 ].…”

Section: Methods Of Betulin Isolationmentioning

confidence: 99%

Methods of Betulin Extraction from Birch Bark

et al. 2022

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Betulin is the most popular of the known triterpenoids of the lupan series. It has valuable pharmacological properties. It exhibits antibacterial, antiviral, antitumor, hypolipidemic and other types of activity. The prospects of using betulin in medicine, pharmacology, and veterinary medicine require the development of effective methods for obtaining it from waste from the woodworking industry. Therefore, the question arises of the need to develop a technology for isolating and purifying triterpenoids from birch bark in large quantities. This review contains a variety of methods for the isolation of betulin. The advantages and disadvantages of the proposed methods are described. The following methods are considered: vacuum sublimation after preliminary alkaline; vacuum pyrolysis; supercritical extraction with carbon dioxide or mixtures of carbon dioxide with various solvents; extraction with organic solvents. Also, the method of microwave is described, it is activation on the example of the Kyrgyz birch (Betula kirghisorum), this is an endemic species that has not been studied before, growing on the territory of the Republic of Kazakhstan.

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