Evaluation and differentiation of the Betulaceae birch bark species and their bioactive triterpene content using analytical FT-vibrational spectroscopy and GC-MS

Abstract

BackgroundAiming to obtain the highest triterpene content in the extraction products, nine bark samples from the forest abundant flora of Apuseni Mountains, Romania were Raman spectroscopically evaluated. Three different natural extracts from Betula pendula Roth birch bark have been obtained and characterized using Fourier transform vibrational spectra.ResultsThis study shows that principal components of the birch tree extract can be rapidly recognized and differentiated based on their vibrational fingerprint band shape and intensity. The vibrational spectroscopy results are supported by the GC-MS data. Based on IR and Raman analysis, one can conclude that all the extracts, independent on the solvent(s) used, revealed dominant betulin species, followed by lupeol.ConclusionsSince Raman measurements could also be performed on fresh plant material, we demonstrated the possibility to apply the present results for the prediction of the highest triterpene content in bark species, for the selection of harvesting time or individual genotypes directly in the field, with appropriate portable Raman equipment.

Identification of Quercus agrifolia (coast live oak) resistant to the invasive pathogen Phytophthora ramorum in native stands using Fourier-transform infrared (FT-IR) spectroscopy

“…While spectroscopy has been used to determine the water status of CLO foliage (Hunt and Rock, 1989 ; Pu et al, 2003 ; Cheng et al, 2011 ) there are no reports of its use for chemically fingerprinting CLO phloem tissue. However, FT-IR spectroscopy has been used to monitor specialized metabolite production in grapevine (Schmidtke et al, 2012 ) and for qualitative and semi-quantitative analysis of birch bark extracts (Cîntă-Pînzaru et al, 2012 ). The technique was also used successfully to identify markers of potato resistance to late blight disease caused by Phytophthora infestans (Mont.)…”

Characterization and in vitro studies of the anticancer effect of oxidized carbon nanotubes functionalized with betulinic acid

“…The bands at 2,915 and 1,383 cm −1 in the spectra of MWCNT-BA could be due to the asymmetric and symmetric C–H stretching vibrations from the methyl and methylene functional groups of BA. 25 Another absorption peak observed at 1,630 cm −1 corresponding to the absorption peak of BA at 1,640 cm −1 could possibly be attributed to the typical stretching band of C=O vibrations of carboxylates. The MWCNT-BA nanocomposite was subjected to three cycles of washing after loading with BA, which means that these bands are from BA, indicating successful interaction of BA in the nanotubes.…”

Sustained Release and Cytotoxicity Evaluation of Carbon Nanotube‐Mediated Drug Delivery System for Betulinic Acid

“…These peaks are attributed to the typical stretching vibrations of C=O and O-H, suggesting the presence of -COOH functional group either from BA molecule or from f-SWCNTs. The peaks at 2916 and 1387 cm −1 imply both the asymmetric and symmetric C-H stretching vibrations which arise from the methyl and methylene group of BA [40], respectively, while the absorption peak at 545 cm −1 could be due to the characteristic absorption of aromatic compound from the benzene rings of BA. Wavenumber (cm −1 ) Figure 3: FTIR transmission spectra of f-SWCNTs-BA and BA.…”