In vitro studies of embryo growth, callus formation and multiple bud induction of Pinus pinea L.

Diamantoglou, S.; Panagopoulos, Ioannis; Munoz-Ferriz, Ana; Rhizopoulou, Sophia

doi:10.1016/s0176-1617(11)80012-x

Cited by 10 publications

(9 citation statements)

References 14 publications

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“…This favorable response may have been due to the fact that the nitrogen content of the modified von Arnold & Eriksson medium is lower than that of MS. The differences between the responses found by Diamantoglou et al (1990) and us with respect to the concentration of the salts and B A could mean that the complete embryos that they used tolerate a greater salt concentration than the isolated cotyledons that we used.…”

Section: Discussionmentioning

confidence: 74%

“…Diamantoglou et al (1990) found that in whole embryo explants the bud production rate was 70% with 5 IxM of BA, falling to 11% with a concentration of 30 ~M of BA. The high ionic concentration ( l l l .…”

Section: Discussionmentioning

confidence: 93%

“…The MS medium, which has the highest nitrogen content of the three basal media used, was the most toxic, presumably because of an excess of ammonium (Boulay 1984;Perez Bermudez & Sommer 1987;Sommer et al 1975). Diamantoglou et al (1990) obtained a higher production of buds with the von Arnold & Eriksson (1981) than with MS medium, in spite of the fact that its total ionic content is higher than that of MS. This favorable response may have been due to the fact that the nitrogen content of the modified von Arnold & Eriksson medium is lower than that of MS.…”

Section: Discussionmentioning

confidence: 99%

“…In vitro culture could overcome these limitations and thus would permit propagation on a large scale. Although there are several reports describing micropropagation methods for different species of Pinus, (David et al 1982;Martinez Pulido et al 1990;Jelaska 1987;Toribio & Pardos 1989), only one report refers to tissue culture of Pinus pinea L. (Diamantoglou et al 1990). …”

Section: Introductionmentioning

confidence: 99%

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In vitro shoot organogenesis from excised immature cotyledons and microcuttings production in Stone Pine

García-Férriz

Serrano

Pardos

1994

Plant Cell Tiss Organ Cult

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Adventitious buds were induced on isolated immature cotyledons of Pinus pinea L. in the presence of benzyladenine (BA). The response to different BA concentrations also depended upon the culture medium used (modified MS, SH and GD). A wide range of BA concentrations (5, 25 or 50 txM) can be applied to the GD and SH media, which are the media with the lower nitrogen content, without damaging effects. In the MS medium, which has the highest nitrogen concentration, the range of BA that can be applied was narrower and the highest BA concentration was lethal. The addition of indolebutyric acid (0.05, 0.25 or 0.5 I~M) to the induction medium, decreased the response of cotyledons. The increase in the concentration of sucrose from 3% to 5% did not increase the number of responding cotyledons. The addition of activated charcoal (0.5 and 3 g 1-1) or indolebutyric acid (1.5 or 3 IxM) did not speed up the elongation of explants. Elongation of the buds produced shoots with two different phenotypes, each phenotype having a different multiplication rate.

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Section: Discussionmentioning

confidence: 74%

Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

In vitro shoot organogenesis from excised immature cotyledons and microcuttings production in Stone Pine

García-Férriz

Serrano

Pardos

1994

Plant Cell Tiss Organ Cult

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“…Regarding micropropagation, there are several reports describing the induction of adventitious shoots from embryonic explants, and even rooting of these microcuttings (Capuana and Giannini 1995;Diamantoglou et al 1990;García-Férriz et al 1994;González et al 1998;Oliveira et al 2003;Valdés et al 2001). However, the advantages of SE-particularly maintenance of regeneration potential by cryopreservation while the testing of clones is in progress (Park 2002)-justify the development of SE protocols for this species.…”

Section: Introductionmentioning

confidence: 99%

Plant regeneration in Stone pine (Pinus pinea L.) by somatic embryogenesis

Carneros¹,

Celestino²,

Klimaszewska

et al. 2009

Plant Cell Tiss Organ Cult

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Regeneration of plants by somatic embryogenesis (SE) was achieved in Stone pine (Pinus pinea), one of the most characteristic tree species of the Mediterranean ecosystem. The initial explants were megagametophytes containing zygotic embryos from five selected half-sib families collected at different dates over 2 consecutive years. Rates of extrusion and initiation of SE differed in both years. However, qualitative patterns were very similar: for most families, the responsive developmental window was from late cleavage polyembryony to early cotyledonary stage. The highest overall mean frequencies of extrusion and SE initiation (7 and 0.9%, respectively, for the five families and the eight 2006 collections) were obtained on a modified Litvay's medium with 9 lM 2,4-D and 4.5 lM BAP, supplemented with L-glutamine and casein hydrolysate. Families showed large differences in frequencies of SE initiation from year to year. Only seven embryogenic lines were induced in 2005, representing three of the five families tested, whereas 34 lines from all the families were obtained in 2006. Proliferation of embryonal masses (EM) was significantly improved when they were subcultured after dispersing in liquid medium and collected on filter paper disks, instead of being subcultured as small clumps. This effect showed a significant interaction with genotype. Several preconditioning treatments and culture media combinations were tested for embryo development and maturation. The high proliferation rate of EM hampered somatic embryo development. However, up to 42 mature embryos from different lines of three of the five families were obtained, 23 of them germinated and seven converted into somatic seedlings.

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Pinus pinea L. (Stone Pine) and Pinus halepensis Mill. (Aleppo Pine)

Diamantoglou¹,

Banilas

1996

Biotechnology in Agriculture and Forestry

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In vitro studies of embryo growth, callus formation and multiple bud induction of Pinus pinea L.

Cited by 10 publications

References 14 publications

In vitro shoot organogenesis from excised immature cotyledons and microcuttings production in Stone Pine

In vitro shoot organogenesis from excised immature cotyledons and microcuttings production in Stone Pine

Plant regeneration in Stone pine (Pinus pinea L.) by somatic embryogenesis

Pinus pinea L. (Stone Pine) and Pinus halepensis Mill. (Aleppo Pine)

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