In vitro shoot organogenesis from excised immature cotyledons and microcuttings production in Stone Pine

Abstract

Adventitious buds were induced on isolated immature cotyledons of Pinus pinea L. in the presence of benzyladenine (BA). The response to different BA concentrations also depended upon the culture medium used (modified MS, SH and GD). A wide range of BA concentrations (5, 25 or 50 txM) can be applied to the GD and SH media, which are the media with the lower nitrogen content, without damaging effects. In the MS medium, which has the highest nitrogen concentration, the range of BA that can be applied was narrower and the highest BA concentration was lethal. The addition of indolebutyric acid (0.05, 0.25 or 0.5 I~M) to the induction medium, decreased the response of cotyledons. The increase in the concentration of sucrose from 3% to 5% did not increase the number of responding cotyledons. The addition of activated charcoal (0.5 and 3 g 1-1) or indolebutyric acid (1.5 or 3 IxM) did not speed up the elongation of explants. Elongation of the buds produced shoots with two different phenotypes, each phenotype having a different multiplication rate.

Micropropagation of Dendrobium densiflorum Lindl. ex Wall. through protocorm-like bodies: effects of plant growth regulators and lanthanoids

“…Data represent means ± SE of roots per shoot in comparison to the basal medium without additives (Table 3). This was similar to the response in stone pine (Garcia-Ferriz et al 1994), however 5.0 mg/l AC treatment could significantly increased frequency of rooting. In D. nobile (Nayak et al 2002), D. aphyllum and D. moschatum (Nayak et al 1997), IBA induced rooting in more than 80% shoots with 2-5 roots per shoot.…”

Shoot organogenesis induction from genetically verified individuals of endangered bog pine (Pinus uncinata subsp. uliginosa)

“…On the full-strength MS medium we found the lowest values of shoot regeneration. Th ese fi ndings agree with results reported by Garcia-Ferriz et al (1994) where the full-strength MS medium was signifi cantly more inhibiting compared to the SH (Schenk, Hildebrandt 1972) medium. In Pinus pinea, the negative eff ect of the MS medium on shoot induction has been attributed to the high nitrogen content, likely to be due to the ammonium presence (Sul, Korban 2004).…”

An improved micropropagation protocol for stone pine (Pinus pinea L.)

“…II), the elongation index (SEC) was significantly lower than on 1 / 2 LPC (81.5) and the multiplication rate was not significantly increased (2.0 ± 0.11). García-Ferriz et al [6] describes this behaviour in P. pinea shoots when BA is applied in shoot development and multiplication stages, besides reporting the appearance of hyperhydric shoots. However, they did not observe any significant increase in the SEC index when shoots are cultured with activated charcoal.…”

“…The production of clonal plants from selected seeds of this coniferous species via organogenesis has been thoroughly studied [4,6,7,14,18]. The propagation system is based on the induction of shoot buds in cotyledonary explants dissected from stone pine embryos and cultured in the presence of a cytokinin (Ck), usually N 6 -benzyladenine (BA).…”