Shoot organogenesis induction from genetically verified individuals of endangered bog pine (Pinus uncinata subsp. uliginosa)

Abstract

ABSTRACT:The objective of this work was to apply selected genetic markers to verify the genetic purity of bog pine (Pinus uncinata subsp. uliginosa) individuals from the Borkovická blata population in order to initiate in vitro cultures from them. Key factors of shoot organogenesis induction from seedling shoot apices were examined. The plastid DNA analysis was used as a molecular-genetic tool. The trnL-trnF region of chloroplast DNA was tested by PCR-RFLP method; furthermore, the chloroplast microsatellite region Pt41093 was also analysed. All the tested individuals from the studied population matched the Pinus uncinata subsp. uliginosa haplotype. In bog pine, regeneration and development of axillary shoots were significantly enhanced in the presence of 5 g·l -1 activated charcoal on the WV5 and WPM (Duchefa) media. The highest proliferation (28-30%) of short shoots (brachyblasts) was observed on the WV5 medium in the absence of benzyladenine (BA) or at its low concentration only (0.1 mg·l -1 ).

Bottlenecks in bog pine multiplication by somatic embryogenesis and their visualization with the environmental scanning electron microscope

“…Moreover, the long-term disruption of the groundwater level and transformation of biotopes after the removal of peat supports Norway spruce and white birch and suppress bog pine. The surviving fragment of bog pine forest consists of old forest vegetation, which is constantly under the invasion of insect pests (Lukášová et al 2012;Vejsadová and Lukášová 2010). Due to rapid decline, bog pine is considered greatly endangered and it is included in the red list of vascular plants of the Czech Republic (Grulich 2012).…”