In vitro spermatogenesis by three-dimensional culture of rat testicular cells in collagen gel matrix

Lee, Jae Ho; Kim, Hyun Joo; Kim, Haekwon; Lee, Sang Jin; Gye, Myung Chan

doi:10.1016/j.biomaterials.2005.12.028

Cited by 114 publications

(78 citation statements)

References 39 publications

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“…However, the mechanisms leading to the development of asthenospermia and abnormal sperm are not yet fully understood. Spermatogenesis is a long and complex process that occurs in the seminiferous tubule of the testis [29], which is regulated by multiple interactions between developing germ cells and the surrounding environment [29]. Spermiogenesis is the final stage of spermatogenesis, during which spermatids mature into motile spermatozoa.…”

Section: Discussionmentioning

confidence: 99%

“…All steps were followed as previously described [29][30][31][32]. Briefly, for the formation embryoid bodies (EBs), the iPS cells were dissociated with 0.125% trypsin-EDTA solution and suspended onto Petri dishes with full medium for 6 days.…”

Section: Methodsmentioning

confidence: 99%

“…All steps were according to the previously described [29][30][31][32]. Briefly, each group cells was washed by PBS on three times, and fixed in 70% ice-cold ethanol, and kept in a freezer more than 48 h. Before flow cytometric analysis, the fixed cells were centrifuged, washed twice with PBS, and resuspended in PI staining solution (Sigma Chemicals) containing 25 mg/mL PI and 40 mg/mL RNase A (Sigma Chemicals) and 0.3% Tween-20 (Sigma-Aldrich).…”

Section: Flow Cytometric Analysis Of Dna Content By Pi Stainingmentioning

confidence: 99%

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MicroRNA-122 Influences the Development of Sperm Abnormalities from Human Induced Pluripotent Stem Cells by Regulating TNP2 Expression

Liu

Huang²,

Liu

et al. 2013

Stem Cells and Development

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Sperm abnormalities are one of the main factors responsible for male infertility; however, their pathogenesis remains unclear. The role of microRNAs in the development of sperm abnormalities in infertile men has not yet been investigated. Here, we used human induced pluripotent stem cells to investigate the influence of miR-122 expression on the differentiation of these cells into spermatozoa-like cells in vitro. After induction, mutant miR-122-transfected cells formed spermatozoa-like cells. Flow cytometry of DNA content revealed a significant increase in the haploid cell population in spermatozoa-like cells derived from mutant miR-122-transfected cells as compared to those derived from miR-122-transfected cells. During induction, TNP2 and protamine mRNA and protein levels were significantly higher in mutant miR-122-transfected cells than in miR-122-transfected cells. High-throughput isobaric tags for relative and absolute quantification were used to identify and quantify the different protein expression levels in miR-122-and mutant miR-122-transfected cells. Among all the proteins analyzed, the expression of lipoproteins, for example, APOB and APOA1, showed the most significant difference between the two groups. This study illustrates that miR-122 expression is associated with abnormal sperm development. MiR-122 may influence spermatozoa-like cells by suppressing TNP2 expression and inhibiting the expression of proteins associated with sperm development.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Flow Cytometric Analysis Of Dna Content By Pi Stainingmentioning

confidence: 99%

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MicroRNA-122 Influences the Development of Sperm Abnormalities from Human Induced Pluripotent Stem Cells by Regulating TNP2 Expression

Liu

Huang²,

Liu

et al. 2013

Stem Cells and Development

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“…Certaines anomalies ont toutefois é té observé es, telle qu'une accé lé ration de la ciné tique de diffé renciation [16], ou bien, l'obtention d'embryons pré sentant de nombreuses anomalies chromosomiques aprè s micro-injection de cellules germinales humaines maturé es in vitro. Enfin, une autre é tude [17] a permis de montrer qu'il é tait possible d'obtenir une prolifé ration et une diffé renciation in vitro des spermatogonies humaines. Aprè s prolifé ration, les spermatogonies ont é té encapsulé es avec des cellules de Sertoli des patients dans de l'alginate de calcium.…”

Section: Maturation In Vitro Des Spermatogonies Souchesunclassified

Gamète mâle… un spermatozoïde ou une spermatide ?

Rives

Milazzo

Arkoun

et al. 2012

Gynécologie Obstétrique & Fertilité

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“…16 In addition, they appear to be capable of being transformed into many different forms, including thin films and porous and gel structures. [17][18][19][20] In this study, we used these biopolymers to form a gel structure as a model system to support cell growth and development. The gel model is transparent; thus, it is readily applicable for most optical-based cellular function assays.…”

mentioning

confidence: 99%

Induction of Ciliated Cells from Avian Embryonic Stem Cells Using Three-Dimensional Matrix

Wang

Wong

Mao

2010

Tissue Engineering Part C: Methods

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We have devised a simple three-dimensional (3D) tissue-culturing method to induce ciliogenesis from avian embryonic stem (ES) cells by using avian fertilized eggs. Unlike the previous reported techniques, this method does not require trypsinization, which would reduce the viability of the cells; it also does not require an airliquid interface to induce ciliogenesis and to maintain the growth of the induced ciliated cells. ES cells seeded and attached on this collagen-coated chitosan 3D gel grew spontaneously and robustly. Following 2 weeks in culture with inhibition of embryoid body formation, cells with noticeable and vigorous beating cilia were observed. We measured the ciliary beat frequencies of these ES-differentiated ciliated cells for 40 days. These results were consistent with all reported measurements made for other species of ciliated cells, including human, from our previous study. These data imply that the cilia of these ES-derived ciliated cells, beating at their intrinsic basal autorhythmic rate, preserve the integrity of the regulatory mechanisms of ciliary beat frequency. In conclusion, we have shown that ES cells cultured in a 3D tissue-engineered scaffold is a promising approach for developing an in vitro cell model that closely mimics the in vivo ciliated cell natural milieu. This cell model can potentially be the source of ciliated cells for cell-based high-throughput screening and discovery of pulmonary drugs.

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In vitro spermatogenesis by three-dimensional culture of rat testicular cells in collagen gel matrix

Cited by 114 publications

References 39 publications

MicroRNA-122 Influences the Development of Sperm Abnormalities from Human Induced Pluripotent Stem Cells by Regulating TNP2 Expression

MicroRNA-122 Influences the Development of Sperm Abnormalities from Human Induced Pluripotent Stem Cells by Regulating TNP2 Expression

Gamète mâle… un spermatozoïde ou une spermatide ?

Induction of Ciliated Cells from Avian Embryonic Stem Cells Using Three-Dimensional Matrix

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