Induction of Ciliated Cells from Avian Embryonic Stem Cells Using Three-Dimensional Matrix

Wang, Yuchi; Wong, L. B.; Mao, Hua

doi:10.1089/ten.tec.2009.0327

Cited by 11 publications

(8 citation statements)

References 43 publications

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“…9,10 Recent studies have also demonstrated that matrix protein composition, 3D scaffolds, and mechanical forces can influence differentiation of embryonic stem cells (ESCs) to lung epithelium. 17,[29][30][31][32] As such, use of different artificial 3D scaffolds is a powerful technique for generating functional lung tissue ex vivo.…”

Section: Discussionmentioning

confidence: 99%

Initial Binding and Recellularization of Decellularized Mouse Lung Scaffolds with Bone Marrow-Derived Mesenchymal Stromal Cells

Daly

Wallis

Borg

et al. 2012

Tissue Engineering Part A

174

253

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Recellularization of whole decellularized lung scaffolds provides a novel approach for generating functional lung tissue ex vivo for subsequent clinical transplantation. To explore the potential utility of stem and progenitor cells in this model, we investigated recellularization of decellularized whole mouse lungs after intratracheal inoculation of bone marrow-derived mesenchymal stromal cells (MSCs). The decellularized lungs maintained structural features of native lungs, including intact vasculature, ability to undergo ventilation, and an extracellular matrix (ECM) scaffold consisting primarily of collagens I and IV, laminin, and fibronectin. However, even in the absence of intact cells or nuclei, a number of cell-associated (non-ECM) proteins were detected using mass spectroscopy, western blots, and immunohistochemistry. MSCs initially homed and engrafted to regions enriched in types I and IV collagen, laminin, and fibronectin, and subsequently proliferated and migrated toward regions enriched in types I and IV collagen and laminin but not provisional matrix (fibronectin). MSCs cultured for up to 1 month in either basal MSC medium or in a small airways growth media (SAGM) localized in both parenchymal and airway regions and demonstrated several different morphologies. However, while MSCs cultured in basal medium increased in number, MSCs cultured in SAGM decreased in number over 1 month. Under both media conditions, the MSCs predominantly expressed genes consistent with mesenchymal and osteoblast phenotype. Despite a transient expression of the lung precursor TTF-1, no other airway or alveolar genes or vascular genes were expressed. These studies highlight the power of whole decellularized lung scaffolds to study functional recellularization with MSCs and other cells.

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Section: Discussionmentioning

confidence: 99%

Initial Binding and Recellularization of Decellularized Mouse Lung Scaffolds with Bone Marrow-Derived Mesenchymal Stromal Cells

Daly

Wallis

Borg

et al. 2012

Tissue Engineering Part A

174

253

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“…We found that the amount of continuous pressure of the media delivered through the vasculature to the re-epithelialized scaffold influenced cell survival, with 20 cm H 2 O being optimal. The fact that the CF and MP protocols were unable to sustain cell viability was a surprising finding, as other investigators have published on cell viability using de-cellularized scaffolds generated by the MP method with A549 cells, fetal pulmonary cells, murine embryonic stem cells, or mesenchymal stem cells [10,16,18–20,23,24,26,27]. Furthermore, other investigators have utilized the MP de-cellularization approach and have been able to support C10 cells for 1 month [18].…”

Section: Discussionmentioning

confidence: 99%

Automated procedure for biomimetic de-cellularized lung scaffold supporting alveolar epithelial transdifferentiation

et al. 2013

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The optimal method for creating a de-cellularized lung scaffold that is devoid of cells and cell debris, immunologically inert, and retains necessary extracellular matrix (ECM) has yet to be identified. Herein, we compare automated detergent-based de-cellularization approaches utilizing either constant pressure (CP) or constant flow (CF), to previously published protocols utilizing manual pressure (MP) to instill and rinse out the de-cellularization agents. De-cellularized lungs resulting from each method were evaluated for presence of remaining ECM proteins and immunostimulatory material such as nucleic acids and intracellular material. Our results demonstrate that the CP and MP approaches more effectively remove cellular materials but differentially retain ECM proteins. The CP method has the added benefit of being a faster, reproducible de-cellularization process. To assess the functional ability of the de-cellularized scaffolds to maintain epithelial cells, intra-tracheal inoculation with GFP expressing C10 alveolar epithelial cells (AEC) was performed. Notably, the CP de-cellularized lungs were able to support growth and spontaneous differentiation of C10-GFP cells from a type II-like phenotype to a type I-like phenotype.

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“…93 However, recent studies have shown that the use of embryonic stem cells can be used to bypass the air-liquid culture step. 94 Nevertheless, this approach requires longer culture periods and does not represent the overall diversity of the cells in the airway epithelium. The cell phenotype can also be affected by changing the substrate to hyaluronan or collagen type IV.…”

Section: Epithelium In Clinical Full Organ/multicellular Tissue-enginmentioning

confidence: 99%

Engineering Functional Epithelium for Regenerative Medicine and In Vitro Organ Models: A Review

Vrana

Lavalle²,

Dokmeci³

et al. 2013

Tissue Engineering Part B: Reviews

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Recent advances in the fields of microfabrication, biomaterials, and tissue engineering have provided new opportunities for developing biomimetic and functional tissues with potential applications in disease modeling, drug discovery, and replacing damaged tissues. An intact epithelium plays an indispensable role in the functionality of several organs such as the trachea, esophagus, and cornea. Furthermore, the integrity of the epithelial barrier and its degree of differentiation would define the level of success in tissue engineering of other organs such as the bladder and the skin. In this review, we focus on the challenges and requirements associated with engineering of epithelial layers in different tissues. Functional epithelial layers can be achieved by methods such as cell sheets, cell homing, and in situ epithelialization. However, for organs composed of several tissues, other important factors such as (1) in vivo epithelial cell migration, (2) multicell-type differentiation within the epithelium, and (3) epithelial cell interactions with the underlying mesenchymal cells should also be considered. Recent successful clinical trials in tissue engineering of the trachea have highlighted the importance of a functional epithelium for long-term success and survival of tissue replacements. Hence, using the trachea as a model tissue in clinical use, we describe the optimal structure of an artificial epithelium as well as challenges of obtaining a fully functional epithelium in macroscale. One of the possible remedies to address such challenges is the use of bottom-up fabrication methods to obtain a functional epithelium. Modular approaches for the generation of functional epithelial layers are reviewed and other emerging applications of microscale epithelial tissue models for studying epithelial/mesenchymal interactions in healthy and diseased (e.g., cancer) tissues are described. These models can elucidate the epithelial/mesenchymal tissue interactions at the microscale and provide the necessary tools for the next generation of multicellular engineered tissues and organ-on-a-chip systems.

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Induction of Ciliated Cells from Avian Embryonic Stem Cells Using Three-Dimensional Matrix

Cited by 11 publications

References 43 publications

Initial Binding and Recellularization of Decellularized Mouse Lung Scaffolds with Bone Marrow-Derived Mesenchymal Stromal Cells

Initial Binding and Recellularization of Decellularized Mouse Lung Scaffolds with Bone Marrow-Derived Mesenchymal Stromal Cells

Automated procedure for biomimetic de-cellularized lung scaffold supporting alveolar epithelial transdifferentiation

Engineering Functional Epithelium for Regenerative Medicine and In Vitro Organ Models: A Review

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