In Vitro Somatic Embryos Multiplication of Eurycoma longifolia Jack using Temporary Immersion System RITA®

Mohd, Noor Madihah; Hafsah, Ja’afar; Zawawi, Dhiya Dalila; Alias, Nadiawati

doi:10.17576/jsm-2017-4606-08

Cited by 8 publications

(2 citation statements)

References 34 publications

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“…Additionally, Dalila et al [ 74 ] found that the highest percentage of SE was produced using modified MS containing 0.1 mg/L zeatin, 0.2 mg/L IBA, and 0.12 mg/L TDZ. Mohd et al [ 75 ] also utilized the mentioned liquid media and varied the immersion frequencies in RITA ® bioreactors. They found that an immersion frequency of 5 min every 4 h produced the highest SE.…”

Section: Tissue Culture Techniques For Mass Propagation In ...mentioning

confidence: 99%

Trends in the Tissue Culture Techniques and the Synthesis of Bioactive Compounds in Eurycoma longifolia Jack—Current Status and Future Perspectives

Sale,

Subramaniam,

Mad’ Atari

2023

Plants

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Over the last two decades, there has been a concerted effort by researchers to mass propagate Eurycoma longifolia and improve the yield of its very important and sought-after anti-cancer and aphrodisiac bioactive compounds. To achieve this, various techniques have been used to mass propagate and improve the yield of these bioactive compounds in tissue cultures. These techniques include the optimization of media conditions and application of various types and combinations of plant growth regulators (PGRs). In addition, some elicitation techniques have been used to improve the synthesis of these bioactive compounds. However, in comparison with other herbal species with similar economic importance, many techniques have not been applied to E. longifolia. Adopting the most recent methodologies would ensure efficiency and sustainability in the in vitro production of bioactive compounds in E. longifolia. Therefore, in this review, we present an up-to-date record on the success stories in the tissue culture techniques and synthesis of bioactive compounds. In addition, we attempted to identify some of the missing links on the road to the effective and sustainable biotechnological utilization of this super important biological resource.

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Section: Tissue Culture Techniques For Mass Propagation In ...mentioning

confidence: 99%

Trends in the Tissue Culture Techniques and the Synthesis of Bioactive Compounds in Eurycoma longifolia Jack—Current Status and Future Perspectives

Sale,

Subramaniam,

Mad’ Atari

2023

Plants

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“…By using cotyledon as an explant, Rodziah and Madihah (2015) successfully produced a high-frequency somatic embryo directly in a short time. More recently, an alternative system of temporary immersion (RITA) has been reported by Mohd et al (2017) for proliferation somatic embryos derived from cotyledons of E. longifolia seeds. However, the direct formation of shoot buds of E. longifolia plant through the organogenesis is not still investigated well.…”

Section: Introductionmentioning

confidence: 99%

Direct Shoot Organogenesis and Clonal Fidelity Confirmation of Tongkat Ali (Eurycoma longifolia) using Molecular Markers

Alttaher¹

2021

IJAB

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Eurycoma longifolia is a medicinally potent plant found in the tropical forest of South-East Asia. Every part of the plant, especially the root is traditionally used as an aphrodisiac, anticancer and anti-inflammatory. E. longifolia is conventionally propagated by seeds but with low germination rate and efficiency. This has made an in vitro propagation of E. longifolia a desirable alternative. Hence, this study reports an effective method of direct organogenesis of shoot. In vitro seedling’s leaves were cultured on Murashige and Skoog (MS) medium containing 1.0 mg L-1 6-benzylaminopurine (BAP), producing 1.8 ± 0.5 shoots per leaf with a regeneration frequency of 68.2%. The shoot buds were directly formed from leaves without intermediate callus formation. To obtain complete plantlets, the shoots were in vitro rooted with an average number of 4.2 ± 0.4 roots per shoot in half-strength MS (½MS) medium supplemented with 0.5 mg L-1 indole-3-butyric acid (IBA). Regenerated plantlets were successfully acclimatized to field conditions with an 85% survival rate. Genetic fidelity of the micropropagated plantlets was evaluated using Simple Sequence Repeat (SSR) and Inter Simple Sequence Repeat (ISSR) analysis. The results showed that the monomorphic banding patterns of in vitro raised plantlets and their mother plant were similar, confirming its homogeneity and the reliability of the multiplication system. © 2021 Friends Science Publishers

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Biotic elicitation at different feeding time in cell suspension cultures of Eurycoma longifolia Jack, a valuable medicinal plant, for enhancement of cytotoxic activity of bioactive compounds against human colon cancer cell line

Kwan

Tan

Hirata

et al. 2021

In Vitro Cell.Dev.Biol.-Plant

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In Vitro Somatic Embryos Multiplication of Eurycoma longifolia Jack using Temporary Immersion System RITA®

Cited by 8 publications

References 34 publications

Trends in the Tissue Culture Techniques and the Synthesis of Bioactive Compounds in Eurycoma longifolia Jack—Current Status and Future Perspectives

Trends in the Tissue Culture Techniques and the Synthesis of Bioactive Compounds in Eurycoma longifolia Jack—Current Status and Future Perspectives

Direct Shoot Organogenesis and Clonal Fidelity Confirmation of Tongkat Ali (Eurycoma longifolia) using Molecular Markers

Biotic elicitation at different feeding time in cell suspension cultures of Eurycoma longifolia Jack, a valuable medicinal plant, for enhancement of cytotoxic activity of bioactive compounds against human colon cancer cell line

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