In vitro snake venom detoxifying action of the leaf extract of Guiera senegalensis

Abubakar, M.S.; Sule, MI; Pateh, U. U.; Abdurahman, E. M.; Haruna, Abdurrashid; Jahun, B. M.

doi:10.1016/s0378-8741(99)00128-2

Cited by 78 publications

(76 citation statements)

References 8 publications

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“…These findings corroborate those of Abubakar et al (2000), Kouamé et al (2009), Shettima et al (2012 and Nartey et al (2012) who reported the presence of these chemical compounds in the three plants. The chemicals found in G. senegalensis leaf extracts were alkaloids, saponosides, coumarins, flavonoids, and tannins.…”

Section: Discussionsupporting

confidence: 90%

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Phytochemical analysis and ovicidal activity of Cassia sieberiana, Guiera senegalensis and Excoecaria grahamii extracts

Traore¹,

Ouédraogo²,

Belemlilga³

et al. 2017

Afr. J. Pharm. Pharmacol.

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Section: Discussionsupporting

confidence: 90%

“…These data corroborate the results previously reported. Indeed, the presence of tannins, flavonoids (Abubakar et al, 2000) and alkaloids (Fiot et al, 2005) was revealed in leaves extracts of this plant. Nacoulma (1996) also reported the presence of these constituents in the leafy stems of G. senegalensis.…”

Section: Discussionmentioning

confidence: 96%

Phytochemical analysis and ovicidal activity of Cassia sieberiana, Guiera senegalensis and Excoecaria grahamii extracts

Traore¹,

Ouédraogo²,

Belemlilga³

et al. 2017

Afr. J. Pharm. Pharmacol.

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“…One possibility is the stimulation of the alternative complement pathway in the chick embryo by components of the extract. This hypothesis is strongly supported by the finding that extracts of G. senegalensis could provide protection against Naja nigricollis venom, which is known to induce tissue necrosis due to its phospholipase content and also to deplete complement (Abubakar et al , 2000). Another antiviral mechanism could be the inhibition of cholesterol biosynthesis by the G. senegalensis gall extract, since the envelope of FPV has been shown to contain cholesterol.…”

Section: Discussionmentioning

confidence: 88%

In ovoinhibition of fowlpoxvirus replication by a gall extract fromGuiera senegalensis

et al. 2005

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Several field isolates of fowlpoxvirus (FPV) from Burkina Faso, West Africa, were isolated and partly evaluated by molecular analysis. In addition, the in ovo antiviral activity against FPV of a gall extract from Guiera senegalensis was determined. Three viral isolates were obtained from suspected fowlpox cases after passage in embryonating chicken eggs and their poxviral identity confirmed by electron microscopy. All isolates were found to be pathogenic for chicks and all grew well in cell culture. Polymerase chain reaction and sequencing of amplicons revealed sequences identical with those of other FPV strains. The most studied isolate was then employed for use in an antiviral assay. An aqueous acetone extract from the galls of G. senegalensis was found to inhibit both virus-induced pock formation and to reduce viral titre in embryonating chicken eggs. The suggested mechanism of action is the activation of the alternative complement pathway and the inhibition of FPV-induced cholesterogenesis in ovo by constituents of the gall extract.

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“…Several plant constituents including flavonoids and terpenoids possess protein binding and enzyme inhibiting properties and also inhibits snake venom phospholipase A 2 of both viper and cobra venom [3]. Polyphenols and tannins are attributable to reduction in enzyme activities [1]. Okonogi et al, [17] suggested that tannins in addition to other plant constituents which are known to unspecifically inactivate proteins to be the likely mechanism involve in detoxifying snake venom.…”

Section: Discussionmentioning

confidence: 99%

Effects of Aqueous Stem Extract of Achyranthes aspera on Bitis arietans Venom Protease and Phospholipase A2 Activity

Nwune¹

2017

AJBIO

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Aqueous stem extract of Achyranthes aspera was investigated for inhibitory activity against Bitis arietans venom protease and phospholipase A 2 activity. The elemental analysis and phytochemical screening of the plant extract were carried out. The activities of protease and phospholipase A 2 (V o ) of the crude Bitis arietans venom was determined and the data obtained was used to estimate K M , V max and K cat . Inhibition studies were carried out using the same procedure except that different concentrations of the extracts (5%, 10%, 15% for protease assay and 0.5%, 0.75%, 10%, 1.25% and 1.5% for phospholipase A 2 assay) were added to the reaction mixture. The result showed that the Bitis arietans venom protease had a V max of 0.062 ± 0.013 µmol/min, K M of 0.496 ± 0.095mg/ml and a K cat of 0.125 ± 0.001min -1. The result also indicates that the Bitis arietans phospholipase A 2 had a V max of 3.27 ± 0.030min -1 , K M of 8.358 ± 0.050 mg/ml and K cat of 0.391 ± 0.002min -. The aqueous stem extract produced a statistically significant (P<0.05) decrease in the V max , K M and K cat of the Bitis arietans venom phospholipase A 2 in a dose dependent manner and a statistically significant (P<0.05) increase in the V max , K M and K cat of Bitis arietans protease in a dose dependent manner. The phytochemical screening revealed the presence of flavonoids, tannins, steroids, saponins and terpenoids in the extract while the elemental analysis revealed the presence of Zn, Cr, Ni, Cd, Mn, Fe and Na. The result suggests that aqueous stem extract of Achyranthes aspera inhibited the Bitis arietans venom phospholipase A 2 in an uncompetitive manner while the protease activity was stimulated by the extracts. It was observed that the use of the stem of Achyranthes aspera may be important in the treatment of snake bites.

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In vitro snake venom detoxifying action of the leaf extract of Guiera senegalensis

Cited by 78 publications

References 8 publications

Phytochemical analysis and ovicidal activity of Cassia sieberiana, Guiera senegalensis and Excoecaria grahamii extracts

Phytochemical analysis and ovicidal activity of Cassia sieberiana, Guiera senegalensis and Excoecaria grahamii extracts

In ovoinhibition of fowlpoxvirus replication by a gall extract fromGuiera senegalensis

Effects of Aqueous Stem Extract of <i>Achyranthes aspera</i> on <i>Bitis arietans</i> Venom Protease and Phospholipase A<sub>2</sub> Activity

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In vitro snake venom detoxifying action of the leaf extract of Guiera senegalensis

Cited by 78 publications

References 8 publications

Phytochemical analysis and ovicidal activity of Cassia sieberiana, Guiera senegalensis and Excoecaria grahamii extracts

Phytochemical analysis and ovicidal activity of Cassia sieberiana, Guiera senegalensis and Excoecaria grahamii extracts

In ovoinhibition of fowlpoxvirus replication by a gall extract fromGuiera senegalensis

Effects of Aqueous Stem Extract of &lt;i&gt;Achyranthes aspera&lt;/i&gt; on &lt;i&gt;Bitis arietans&lt;/i&gt; Venom Protease and Phospholipase A&lt;sub&gt;2&lt;/sub&gt; Activity

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Effects of Aqueous Stem Extract of <i>Achyranthes aspera</i> on <i>Bitis arietans</i> Venom Protease and Phospholipase A<sub>2</sub> Activity