In vitro skin model for the evaluation of burn healing drug delivery systems

Barbalho, Geisa N.; Matos, Breno N.; Santo, Maria Eduarda L. Espirito; Silva, Vinicius R.C.; Chaves, Sacha Braun; Gelfuso, Guilherme M.; Cunha-Filho, Marcílio

doi:10.1016/j.jddst.2021.102330

Cited by 7 publications

(2 citation statements)

References 25 publications

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“…Briefly, quintuplicates of previously prepared porcine skin samples were used (5.7-cm 2 ). The burnt skin sample simply consisted of porcine skin that had been burnt for 7 sec by a hot plate at 85 • C [28]. Permeation was carried out for 24 h by adding 2 mL of NC-CH and NC onto the skin surface.…”

Section: Skin Penetrationmentioning

confidence: 99%

Skin Regenerative Potential of Cupuaçu Seed Extract (Theobroma grandiflorum), a Native Fruit from the Amazon: Development of a Topical Formulation Based on Chitosan-Coated Nanocapsules

et al. 2022

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Scarless skin regeneration is a challenge in regenerative medicine. Herein, we explore the regenerative potential of a Cupuaçu seed extract (Theobroma grandiflorum) to develop an innovative skin regeneration formulation based on chitosan-coated nanocapsules. Cupuaçu seed extract significantly stimulated cell proliferation and migration. A reparative gene expression profile could be verified following extract treatment, which included high levels of MKI67, a cellular proliferation marker, and extracellular matrix genes, such as ELN and HAS2, which code for elastin and hyaluronic acid synthase 2. Formulations with Cupuaçu seed extract successfully entrapped into nanocapsules (EE% > 94%) were developed. Uncoated or coated nanocapsules with low-molecular-weight chitosan presented unimodal size distribution with hydrodynamic diameters of 278.3 ± 5.0 nm (PDI = 0.18 ± 0.02) and 337.2 ± 2.1 nm (PDI = 0.27 ± 0.01), respectively. Both nanosystems were physically stable for at least 120 days and showed to be non-irritating to reconstructed human epidermis. Chitosan coating promoted active penetration into undamaged skin areas, which were still covered by the stratum corneum. In conclusion, the present study demonstrated for the first time the biotechnological potential of the frequently discarded Cupuaçu seed as a valuable pharmaceutical ingredient to be used in regenerative skin products.

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Section: Skin Penetrationmentioning

confidence: 99%

Skin Regenerative Potential of Cupuaçu Seed Extract (Theobroma grandiflorum), a Native Fruit from the Amazon: Development of a Topical Formulation Based on Chitosan-Coated Nanocapsules

et al. 2022

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“…Optical Coherence Tomography (OCT) is a non-invasive, non-destructive optical imaging technique that uses a low coherence interferometer to obtain real-time crosssectional images and three-dimensional images of a biological tissue sample. It was first developed in 1991 by David Huang (17) and OCT is notably used in biomedical applications including ophthalmology and dermatology (18)(19)(20)(21)(22)(23)(24)(25)(26). It also extends to industrial, pharmaceutical, and poultry applications (27)(28)(29)(30)(31).…”

Section: Introductionmentioning

confidence: 99%

Leaf Structure and Attenuation Coefficients of Citrofortunella Microcarpa Leaves Using a Portable TD-OCT System

Galvez¹,

Lara²,

Mandia³

et al. 2022

ECS Trans.

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A non-invasive and simple point measurement using a portable TD-OCT system was used to measure the leaf structure and attenuation coefficient of healthy and unhealthy leaves of a Citrofortunella Microcarpa plant. Assuming homogeneity within the layers, the attenuation coefficient was obtained by linear fitting the logarithm of the A-scan or depth profile of the leaf. The attenuation coefficients of the epidermal and palisade layers of the healthy leaves were 27.07/mm and 15.36/mm, respectively, and for the unhealthy leaves they were 21.82/mm and 8.01/mm, respectively. Comparing the A-scan profiles obtained for the healthy and unhealthy leaves, a mixing of the epidermal and palisade layers within the unhealthy leaves was observed. These observations and the decrease in the attenuation coefficients of the unhealthy leaves are indications of deterioration in the photosynthetic rate that enhances the growth and development of the plants which can provide early detection of sickness benefitting Filipino farmers.

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Iontophoresis application for drug delivery in high resistivity membranes: nails and teeth

Andrade

Miranda

Cunha-Filho

et al. 2022

Drug Deliv. and Transl. Res.

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In vitro skin model for the evaluation of burn healing drug delivery systems

Cited by 7 publications

References 25 publications

Skin Regenerative Potential of Cupuaçu Seed Extract (Theobroma grandiflorum), a Native Fruit from the Amazon: Development of a Topical Formulation Based on Chitosan-Coated Nanocapsules

Skin Regenerative Potential of Cupuaçu Seed Extract (Theobroma grandiflorum), a Native Fruit from the Amazon: Development of a Topical Formulation Based on Chitosan-Coated Nanocapsules

Leaf Structure and Attenuation Coefficients of Citrofortunella Microcarpa Leaves Using a Portable TD-OCT System

Iontophoresis application for drug delivery in high resistivity membranes: nails and teeth

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