In-vitro singlet oxygen threshold dose at PDT with Radachlorin photosensitizer

Клименко, В. В.; Shmakov, Stanislav V.; Kaydanov, N. E.; Knyazev, Nickolay A.; Kazakov, Nikita; Rusanov, A. A.; Bøgdanov, A.; Dubina, Michael

doi:10.1117/12.2286457

Cited by 3 publications

(7 citation statements)

References 10 publications

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“…As shown by S-formulas, Eq. ( 5 We have demonstrated that our formulas predict very well the measured results of Klimenko [3]. However, the accuracy of our formulas will require accurate measurement of the parameters involved, such as the rate constant (K), the quantum yield (q), the molar extinction coefficient of the initiator (a') and the photolysis product etc.…”

Section: Efficacy Improvementmentioning

confidence: 65%

“…We note that Eq. (1) was also presented by Gkigkitzis et al [6], Zhu et al [7,8] and Klimenko et al [3] for the anti-cancer kinetics. However, they have assumed a constant light intensity, i.e., A'(z,t) is a constant in Eq.…”

Section: Kinetcis and Modelingmentioning

confidence: 67%

“…Therefore, the threshold light dose and singlet oxygen dose play the important role in PDT for anticancer. Recently, the singlet oxygen threshold dose, and the dosedependence cell viability curves of human cancer cells of K562 and Hela after red-light irradiation of Radachlorin were reported in vivo [3].…”

Section: Introductionmentioning

confidence: 99%

“…This study will theorize the role of concentration of PS and oxygen, rate constant, oxygen external source term, light dose, intensity and exposure time in the efficacy and threshold dose of anti-cancer via oxygen-mediated type-II mechanism. We will also analyze the measured in vitro singlet oxygen threshold dose reported by Klimenko et al [3]. Both analytic formulas (for the first time) and numerical calculations will be presented.…”

Section: Introductionmentioning

confidence: 99%

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Analysis the Role of Oxygen, Light Intensity, Threshold Dose and Efficacy Improvement of Anti-Cancer via Type-II Photodynamic Therapy

Lin¹,

Chen

Liu³

2018

NACS

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The role of oxygen, cell killing threshold dose and efficacy improvement of anti-cancer via type-II phototherapy are analyzed by both analytic formulas and numerical data. Higher initial photoinitiator (PS) concentration (C 0 ) and/or light intensity (I 0 ) has a faster efficacy, due to faster depletion of PS and oxygen concentration. Without the external oxygen supply, higher C 0 or I 0 produces more efficient singlet oxygen, resulting a higher transient efficacy, but they reach the same steady-state value when oxygen is completely depleted. With the external oxygen supply, higher efficacy (with no steady-state limitation), or lower cell viability are achieved. The overall efficacy may be enhanced by resupply of PS or oxygen during the light exposure.

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Section: Efficacy Improvementmentioning

confidence: 65%

Section: Kinetcis and Modelingmentioning

confidence: 67%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Analysis the Role of Oxygen, Light Intensity, Threshold Dose and Efficacy Improvement of Anti-Cancer via Type-II Photodynamic Therapy

Lin¹,

Chen

Liu³

2018

NACS

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“…predicted feature has been clinically demonstrated in ophthalmic system for CXL [27]. Anti-cancer via oxygen-mediated type-II mechanism has been clinically studied [32,33], where the cytotoxic effect of photodynamic therapy (PDT) to tumor tissue is resulted by the generation of singlet oxygen. Efficacy of PDT is mainly influenced by: the concentration of PS drug accumulated into the cells, molecular oxygen in tissue, the light dose, intensity and dose (fluence) [34].…”

Section: Analysis Of Experimentsmentioning

confidence: 99%

Modeling the Dynamic Concentration Profiles and Efficacy of Type-I and Type-II Photopolymerization

J¹,

Cheng²,

Chen³

et al. 2018

Preprint

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The kinetics and efficacy profiles of photoinitiated polymerization are theoretically presented. For the same dose, lower light intensity achieves a higher steady-state-efficacy (SSE) in type-I; in contrast, type-II has an equal SSE. Higher light intensity has a faster rising efficacy, due to faster depletion of photoinitiator (PS) concentration. However, type-II process is also affected by the available oxygen. Higher light intensity produces more efficient singlet oxygen, resulting a higher transient efficacy, in which all intensities reach the same SSE when oxygen is completely depleted. With external oxygen, type-II efficacy increases with time, otherwise, it is governed only by the light dose, i.e., same dose achieves same efficacy. Moreover, type-II has an efficacy follows Bunsen Roscoe law (BRL), whereas type-I follows non-BRL. The measured type-I efficacy and gelation profile are analyzed by our analytic formulas. Schematics of the photocrosslinking stage defined by the availability of oxygen is developed, where both type-I and –II coexist until the oxygen is depleted. The overall efficacy may be enhanced by resupply of PS or oxygen during the light exposure. The roles of light dose and PS concentration on the efficacy of photoinitiated polymerization should be are governed a new concept of a volume efficacy (Ve), defined by the product of the crosslink (or gelation) depth (CD) and local [efficacy].

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Empirical Modeling of Cancer Cell Viability in Oxygen-Mediated Photodynamic Therapy

Chen¹,

Lin²,

Liu³

2019

J Cancer Sci Clin Ther

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The dynamic roles of photosensitizer (PS) concentration and light intensity were measured and analyzed empirically. The efficacy of photodynamic therapy (PDT) and cell viability (CV) are measured (in vitro) and analyzed by analytic and numerical modeling. For a fixed PS concentration, CV is a nonlinear deceasing function of light intensity and exposure time; for a fixed light intensity, higher PS concentration achieves higher efficacy or smaller CV (at steady-state), in consistent to our analytic formulas. The anticancer efficacy may be improved by various strategies such as resupply of Ce6, external oxygen, or stabilizing the singlet oxygen (with increased lifetime).

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In-vitro singlet oxygen threshold dose at PDT with Radachlorin photosensitizer

Cited by 3 publications

References 10 publications

Analysis the Role of Oxygen, Light Intensity, Threshold Dose and Efficacy Improvement of Anti-Cancer via Type-II Photodynamic Therapy

Analysis the Role of Oxygen, Light Intensity, Threshold Dose and Efficacy Improvement of Anti-Cancer via Type-II Photodynamic Therapy

Modeling the Dynamic Concentration Profiles and Efficacy of Type-I and Type-II Photopolymerization

Empirical Modeling of Cancer Cell Viability in Oxygen-Mediated Photodynamic Therapy

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