In vitro Simulation of Thermotherapy for Benign Prostatic Hyperplasia by Fourier Transform Infrared Microspectroscopy Combined with Differential Scanning Calorimetry

Lin, Shan‐Yang; Hsu, Hueih-Shing; Lin, Alex Tong‐Long; Liang, Run-Chu

doi:10.1159/000283053

Cited by 3 publications

(4 citation statements)

References 18 publications

(21 reference statements)

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“…This suggested that the higher content of collagen was observed in the stromal tissue than in the epithelial tissue of the proliferous BPH. [154,155] The thermotherapy has been successfully used to treat the BPH patients by using a transurethral prostatic heating device. We had used a novel system by combining the microscopic FT-IR spectroscopy and differential scanning calorimetry (DSC) to non-isothermally (from 25 to 60 • C) or isothermally (47 • C, 3 h) simulate the clinical BPH thermotherapy, in vitro.…”

Section: Detection Of Benign Prostatic Hyperplasiamentioning

confidence: 99%

FT‐IR and Raman vibrational microspectroscopies used for spectral biodiagnosis of human tissues

Lin¹,

Li²,

Cheng³

2007

Journal of Spectroscopy

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106

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Fourier transform infrared (FT-IR) and Raman vibrational microspectroscopies used for biomedical diagnosis of human tissues are reviewed from basic principle to biological applications. The advantages and disadvantages of both vibrational microspectroscopies are compared to highlight their efficiency and adaptability for noninvasively investigating the chemical compositions of ultrastructual human tissues at different disease states. Biochemical fingerprints applied to the biological samples by using FT-IR and Raman microspectroscopies are illustrated. The spectral biodiagnoses of several diseased human tissues such as ophthalmic disorders (risk factors-induced cataractous lens capsules and lens, lens and corneal calcifications, opacification and contamination of intraocular lens, vitreous asteroid bodies), alcohol-disordered human gastric mucosa, skin disorders (cancer and calcification), brain tumors (pituitary adenomas and astrocytomas), genetic hair roots disorder (glucose-6-phosphate dehydrogenase deficiency, phenylketonuria and congenital hypothyroidism), benign prostatic hyperplasia, and interstitial cystitis investigated by both vibrational microspectroscopies in our laboratory are introduced.

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Section: Detection Of Benign Prostatic Hyperplasiamentioning

confidence: 99%

FT‐IR and Raman vibrational microspectroscopies used for spectral biodiagnosis of human tissues

Lin¹,

Li²,

Cheng³

2007

Journal of Spectroscopy

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106

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“…In our previous study (9), a thermally induced conversion from a -helix to b -structure in the epithelium or stroma of BPH tissue was found by heating the sample from 25 to 60°C with this microscopic FTIR and DSC combined system. In our hospital, however, a few hundred BPH patients have been successfully treated using a transurethral prostatic heating device controlling the heating element to 47°C for a single 3 h section.…”

mentioning

confidence: 87%

“…There was no significant difference before or after light pressing. The disc was then placed on the DSC microscopy cell (FP 84, Mettler, Switzerland), which was set in the FTIR microscopic spectrometer (Micro FTIR-200, Jasco, Japan) equipped with an MCT detector (7)(8)(9)11). The temperature of the DSC microscopy cell was monitored with a central processor (FT80HT, Mettler, Switzerland).…”

Section: Microscopic Ftir and Dsc Studymentioning

confidence: 99%

Integrality of Benign Prostatic Hyperplasia Tissues After Transurethral Thermotherapy Evidenced by Transmittance Fourier Transform Infrared Spectroscopy Combined with Thermal Analyzer

Lin¹,

Hsu²,

Liang³

et al. 1998

Scandinavian Journal of Urology and Nephrology

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Transmittance Fourier transform infrared microspectroscopy combined with differential scanning calorimetry was used to simulate the clinically transurethral thermotherapy of benign prostatic hyperplasia (BPH) by in vitro isothermal determination. The simulative study to the thermotherapy of epithelium or stroma in BPH tissue was performed at 47 degrees C for 3 h, like clinical therapy. Whether thermal treatment can induce changes in the secondary structure of epithelium or stroma in BPH tissue was investigated. The results indicate that the epithelium and stroma in BPH tissue had different protein secondary structures, due to the different compositions of the epithelium and stroma. No significant change was evidenced in secondary structure for each sample either before or after isothermal study, suggesting the integrality and safety of BPH thermotherapy in a 47 degrees C for 3 h treatment course.

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“…The histological, morphological, and biochemical properties of both compartments in BPH tissue have been considerably investigated [1, 8^10]. We have used a microscopic Fourier transform infrared spectroscopy combined with differential scanning calorimetry (microscopic FT-IR/DSC system) to in vitro and isothermally simulate the thermotherapy (47¯C, 3 h) for BPH, and found no significant change in secondary structure for each isolated compartment [11]. On the other hand, the secondary conformation of isolated epithelium and stroma of BPH tissue could change slightly from a-helix to b-structure with the nonisothermal treatment [12].…”

mentioning

confidence: 99%

Ultrastructural and Biophysical Studies on Protein Conformations of Epithelium and Stroma in Benign Prostatic Hyperplasia Before and After Transurethral Resection of the Prostate

Hsu¹,

Lin²,

Li³

et al. 2002

Ultrastructural Pathology

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The alterations in protein conformation of epithelium and stroma in human benign prostatic hyperplasia (BPH) undergoing prostatic needle biopsy (PNB) and transurethral resection of the prostate (TURP) were isolated in vitro and investigated using attenuated total reflectance (ATR)/Fourier transform infrared (FT-IR) microspectroscopy. Second-derivative spectral analysis and a curve-fitting program were used to verify and obtain the protein secondary conformation and composition of the samples. TURP uses an electric cutting heat but PNB is a nonheating process. The results indicate a predominantly higher proportion of beta-sheet conformation of the epithelium or stroma in BPH tissue at 1633 or 1630 cm(-1) in the amide I band, but after treatment with TURP, both maximum peaks shifted to 1644 or 1646 cm(-1) due to random coil structure, respectively. This conversion suggests that the heat generated by the cutting loop could increase the temperature in the tissue surrounding the prostate, thereby modifying the protein conformational structure of epithelium and stroma in BPH tissue.

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In vitro Simulation of Thermotherapy for Benign Prostatic Hyperplasia by Fourier Transform Infrared Microspectroscopy Combined with Differential Scanning Calorimetry

Cited by 3 publications

References 18 publications

FT‐IR and Raman vibrational microspectroscopies used for spectral biodiagnosis of human tissues

FT‐IR and Raman vibrational microspectroscopies used for spectral biodiagnosis of human tissues

Integrality of Benign Prostatic Hyperplasia Tissues After Transurethral Thermotherapy Evidenced by Transmittance Fourier Transform Infrared Spectroscopy Combined with Thermal Analyzer

Ultrastructural and Biophysical Studies on Protein Conformations of Epithelium and Stroma in Benign Prostatic Hyperplasia Before and After Transurethral Resection of the Prostate

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