FT-IR and Raman vibrational microspectroscopies used for spectral biodiagnosis of human tissues

Abstract

Fourier transform infrared (FT-IR) and Raman vibrational microspectroscopies used for biomedical diagnosis of human tissues are reviewed from basic principle to biological applications. The advantages and disadvantages of both vibrational microspectroscopies are compared to highlight their efficiency and adaptability for noninvasively investigating the chemical compositions of ultrastructual human tissues at different disease states. Biochemical fingerprints applied to the biological samples by using FT-IR and Raman microspectroscopies are illustrated. The spectral biodiagnoses of several diseased human tissues such as ophthalmic disorders (risk factors-induced cataractous lens capsules and lens, lens and corneal calcifications, opacification and contamination of intraocular lens, vitreous asteroid bodies), alcohol-disordered human gastric mucosa, skin disorders (cancer and calcification), brain tumors (pituitary adenomas and astrocytomas), genetic hair roots disorder (glucose-6-phosphate dehydrogenase deficiency, phenylketonuria and congenital hypothyroidism), benign prostatic hyperplasia, and interstitial cystitis investigated by both vibrational microspectroscopies in our laboratory are introduced.

Application of Fourier Transform Infrared Spectroscopy for Tumor Diagnosis

“…The most significant differences in the spectrums of normal and cancerous tissues and cells have been observed in the wavenumber region between 400 and 4000 cm -1 . thus, the mid-infrared region was reported as a major cancer diagnosis indicator (6,7,13,15,19,21,23,25,26,34,38,39,43,46,51,52,62).…”

First FT-Raman and 1H NMR comparative investigations in ring opening metathesis polymerization

“…Raman has obvious advantages over the IR absorption spectroscopy, a technique which along with Raman accomplishes the full vibrational characterization of a molecule [23][24][25][26]. The scattering FT-Raman is usually less complicated, and the interpretation of data is more straightforward, yet the Raman signal is frequently weaker than in FT-IR.…”

Evaluation of catheter-induced tribological damage to porcine aorta using infra-red spectroscopy

“…However, there are large discrepancies in the literature over the spectral changes in the above-defined regions which occur due to damage [30,[34][35][36][37][38]. This is likely attributable to the fact that this research involves heterogeneous, live biological tissue and samples extracted from different body locations under various conditions which may have introduced spectral differences between the observations reported in the literature.…”