In vitro shoot formation and enrooted mini-corm production by direct organogenesis in saffron (crocus sativus L.)

Lagram, Khalid; Merzougui, Soumaya El; Boudadi, Imane; Caid, Mohamed Ben El; Boullani, Rachida El; Mousadik, Abdelhamid El; Serghini, Mohammed Amine

doi:10.1007/s42535-023-00639-9

Cited by 4 publications

(9 citation statements)

References 21 publications

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“…Therefore, a thorough sterilization process is indispensable to eliminate contaminants from the geophytic storage organ explants. In the study conducted by Lagram et al [128], a protocol was employed for the preparation of saffron daughter corms (Crocus sativus L.). The corms were subjected to a series of treatments, including a 30 min immersion in tap water, gentle brushing with Tween-20, a 1 min exposure to 80% ethanol, a 20 min treatment with freshly prepared mercuric chloride (HgCl 2 ) at a concentration of 0.2%, and, finally, rinsing four times for 4 min each with sterile distilled water.…”

Section: Stage 1: Establishment Of Aseptic Culturementioning

confidence: 99%

“…Additionally, this study showed that the type of carbohydrate added to the nutrient solution had a significant effect on the shoot proliferation of the tulip cultivar; the highest multiplication of the shoots (number of shoots for one starting clump) was achieved with sucrose (34.33 shoots/per clump) and glucose (35.88 shoots/per clump) as carbohydrates. Lagram et al [128], used excised mother corm buds under different 6-benzyl aminopurine (BAP), 2,4-Dichlorophenoxy acetic acid (2.4-D) and NAA concentrations in MS medium. The highest bud sprouting (96.67%) and shoot growth (8.87 cm) were obtained from 1 mg/L BAP and 1 mg/L NAA.…”

Section: Stage 2: Multiplicationmentioning

confidence: 99%

“…Additionally, soluble sugars in bulbs were stimulated by darkness and blue light. Lagram et al [128] used different sucrose, IBA, and NAA concentrations on corm production and root regeneration in 1 2 MS. Furthermore, they considered the photoperiod effect (16 h/8 h and dark conditions) on the parameters.…”

Section: Stage 3: Bulb Growthmentioning

confidence: 99%

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A Review on Flower Bulb Micropropagation: Challenges and Opportunities

Yasemin,

Beruto

2024

Horticulturae

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This comprehensive review scrutinizes tissue culture and micropropagation methodologies in geophytes, focusing on bulbous plants. The examination encompasses key stages, including somatic embryogenesis, bulb growth, dormancy breaking, and planting. Studies underscore the pivotal role of plant growth regulators (PGRs) in plant regeneration and bulb growth. Bioreactor systems for healthy plant regeneration, rooting methods, acclimatization strategies, and considerations for ex vitro survival are elucidated. The review also delves into somaclonal variation dynamics and acknowledges the burgeoning field of gene editing, particularly Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) studies, as a promising avenue for enhancing valuable compound content in geophytes. In addition to addressing challenges in flower bulb micropropagation, this review briefly highlights emerging opportunities, including the potential integration of artificial intelligence (AI) to optimize culture conditions, predict growth parameters, and enhance efficiency in bulb production. The conclusion emphasizes the necessity of a multifaceted approach integrating biochemistry, physiology, and molecular biology to address existing challenges and improve tissue culture protocols for diverse geophyte species. This review article also intends to highlight how tissue culture techniques could contribute to the development and valorization of flower bulbs in today’s scenario of the ornamental industry.

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Section: Stage 1: Establishment Of Aseptic Culturementioning

confidence: 99%

Section: Stage 2: Multiplicationmentioning

confidence: 99%

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A Review on Flower Bulb Micropropagation: Challenges and Opportunities

Yasemin,

Beruto

2024

Horticulturae

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“…The conventional in vitro propagation using the semisolid medium is proposed as a system that increases the number of corms produced [49][50][51], but it is often a cost-labor method. Consequently, the introduction of semi-automation by plant bioreactors for in vitro propagation has been proposed as a strategy to reduce production costs [52][53][54].…”

Section: In Vitro Propagation Of Saffronmentioning

confidence: 99%

Saffron In Vitro Propagation: An Innovative Method by Temporary Immersion System (TIS), Integrated with Machine Learning Analysis

Tarraf,

İzgü,

Şimşek

et al. 2024

Horticulturae

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The propagation of Crocus sativus L. relies exclusively on corm multiplication. As underground storage organs, corms are susceptible to a wide range of pathogens, environmental stresses, and diseases, making traditional propagation methods often ineffective with the loss of valuable material. In vitro propagation offers an alternative for the saffron culture under controlled conditions. In particular, the innovative application of the Temporary Immersion System (TIS) represents a technological advancement for enhancing biomass production with a reduction in operational costs. The current study utilized the Plantform™ bioreactor to propagate in vitro saffron corms from the ‘Abruzzo’ region (Italy), integrating machine learning models to assess its performance. The evaluation of saffron explants after 30, 60, and 90 days of culture showed a marked improvement in growth and microcorm production compared to conventional in vitro culture on semisolid medium, supported by the machine learning analysis. Indeed, the Random Forest algorithm revealed a predictive accuracy with an R2 value of 0.81 for microcorm number, showcasing the capability of machine learning models to forecast propagation outcomes effectively. These results confirm that applying TIS in saffron culture could lead to economically viable, large biomass production within a controlled environment, irrespective of seasonality. This study represents the first endeavor to use TIS technology to enhance the in vitro propagation of saffron in conjunction with machine learning, suggesting an innovative approach for cultivating high-value crops like saffron.

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“…Morocco is considered the fourth largest producer of saffron spice in the world [12], with an average of 5-6 tons per year. However, climatic changes and stress linked to the soil of Taliouine, which is the main cultivation region in Morocco, and the limited healthy saffron plants are the primary challenging factors for large-scale production [4,13]. Because Moroccan saffron is only produced in open fields, there is a need to improve the rates of propagation with alternative methods.…”

Section: Introductionmentioning

confidence: 99%

Propagation of Saffron (Crocus sativus L.) Using Cross-Cuttings under a Controlled Environment

El Merzougui,

Boudadi,

Lachguer

et al. 2024

IJPB

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Saffron (Crocus sativus L.) is a valuable geophyte plant and one of the most expensive spices in the world. Recently, the demand for saffron spice has increased in worldwide markets owing to its enormous application and value. However, the production of saffron is limited by the vegetative propagation technique and the limited number of high-quality corms planted. Furthermore, climatic changes, notably increasing temperatures, negatively influence saffron multiplication and growth. Thus, it is important to develop alternative cultivation and propagation techniques for saffron under a controlled environment, which could ensure an increase in saffron yield and avoid the negative impact of climatic changes. The present study aimed to develop an alternative method for vegetative propagation of Crocus sativus under controlled conditions. The effect of different cross-cuttings, including basal cuttings (BCs) and top-to-bottom cuttings (CTBs), was evaluated on shoot, leaf, flower, and daughter corm production. All the growth parameters examined were influenced by the cutting treatment applied. The results showed that the highest number of shoots formed was obtained by BCs and CTBs, with an average of 6.68 and 5.47 shoots per corm, respectively, compared to the control with an average of 2.70 shoots per corm. The cutting treatment positively affected the formation of daughter corms in which, the high mean number of corms recorded was obtained by the BC treatment. Meanwhile, the lower size of the daughter corms was obtained after the cross-cutting treatment. This is the first report that provides an alternative propagation for saffron using a controlled environment, which could help to improve the production of saffron.

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In vitro shoot formation and enrooted mini-corm production by direct organogenesis in saffron (crocus sativus L.)

Cited by 4 publications

References 21 publications

A Review on Flower Bulb Micropropagation: Challenges and Opportunities

A Review on Flower Bulb Micropropagation: Challenges and Opportunities

Saffron In Vitro Propagation: An Innovative Method by Temporary Immersion System (TIS), Integrated with Machine Learning Analysis

Propagation of Saffron (Crocus sativus L.) Using Cross-Cuttings under a Controlled Environment

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