In Vitro Selection of Foeniculum vulgare for Salt Tolerance

KHORAMI, Azita; Safarnejad, Abbas

doi:10.15835/nsb325806

Cited by 8 publications

(4 citation statements)

References 37 publications

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“…All these alterations presumably enhanced and produced a balanced basal medium composition that was high suitable for callus formation. Obtaining a balanced medium composition was also necessary to successfully establish an in vitro culture of Gatharanthus roseus (Morard and Henry 1998), callus growth of Prunus domestica L. (Nowak et al 2007), regeneration of Dianthus henteri (Cristea et al 2010), and callus induction and regeneration of Foeniculum vulgare (Khorami and Safarnejad 2011). Specific basal medium for callus initiation in anther culture was also reported on other plants.…”

Section: Discussionmentioning

confidence: 99%

New basal media for half-anther culture of Anthurium andreanum Linden ex André cv. Tropical

Winarto¹,

Rachmawati²,

Silva

2011

Plant Growth Regul

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A successful protocol for high frequency callus induction and plant regeneration from Anthurium andreanum Linden ex André cv. Tropical half-anthers is described. Different variables using Winarto and Teixeira and Murashige and Skoog basal media supplemented with several plant growth regulators [2,4-dichlorophenoxy acetic acid (0.1-1.0 mg/l), a-naphthalene acetic acid (0.01-0.2 mg/l), thidiazuron (0.5-2.0 mg/l), 6-benzylaminopurine (0.5-1.0 mg/l), and kinetin (0.5-1.0 mg/l)] were tested for their ability to induce high frequency callusing in half-anthers, indirect regeneration and rooting of shoots. Basal medium, as well as the combination and concentration of hormones applied, had a significant effect on callus formation, shoot regeneration and adventitious root formation. Winarto and Teixeira-1, an original basal medium containing 0.01 mg/l a-naphthalene acetic acid, 0.5 mg/l thidiazuron and 1.0 mg/l 6-benzylaminopurine was suitable for callus formation while an improved basal medium i.e., New Winarto-Teixeira-3 supplemented with 0.25 mg/l 2,4-dichlorophenoxy acetic acid, 0.02 mg/l a-naphthalene acetic acid, 1.5 mg/l thidiazuron and 0.75 mg/l 6-benzylaminopurine enhanced callus formation. High shoot regeneration and multiplication was also possible on New Winarto-Teixeira-3. Shoots formed a strong adventitious root system on New Winarto-Teixeira-3 containing 0.2 mg/l a-naphthalene acetic acid and 1.0 mg/l kinetin. Plantlets that varied in size and performance were successfully acclimatized and adapted to ex vitro conditions. Cytological analysis of 180 acclimatized-plantlets ex vitro revealed that 34 were haploid (n = 14-18), 15 aneuploid (n = 20-26), 126 diploid (n = 28-34) and 5 triploid (n = 45-57). The potential use of this protocol for developing half-anther culture of other Anthurium species or cultivars is discussed.

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Section: Discussionmentioning

confidence: 99%

New basal media for half-anther culture of Anthurium andreanum Linden ex André cv. Tropical

Winarto¹,

Rachmawati²,

Silva

2011

Plant Growth Regul

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“…La versatilidad de estas técnicas permite su aplicación en la obtención de material potencialmente resistente a hongos patógenos (Rugini, Gutiérrez-Pesce, Silvestri, Lamancusa, Chiavaioli, Vittori & De Pace, 2014); Valencia, Pascual y Delfín, 2014), metales pesados (Priyadarsini, Sahoo & Rout, 2015), tolerancia a la sal (Khorami & Safarnejad, 2011) y a la sequía (Mahmood, Razzaq, Rasheed, Qayyum & Ahmad, 2014) y su combinación con la mutagénesis (Koch, Ramgareeb, Stuart, Snyman & Watt, 2012). Empero, tienen una importante debilidad: no todos los caracteres deseables que se expresan in vivo lo hacen también en las células, tejidos y plantas cultivados in vitro.…”

Section: Figura 2 Esquema General De Selección In Vitro En Tejidos Vunclassified

Perspectivas futuras e impacto social de las biotecnologías vegetales

et al. 2017

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RESUMENLas investigaciones en biotecnología vegetal han conducido a la introducción en la agricultura de numerosos productos científicos, entre los que se destacan el mejoramiento genético y la multiplicación acelerada de especies vegetales, la conservación de germoplasma y las plantas transgénicas. Al mismo tiempo, se ha mantenido un constante debate sobre los beneficios y costos sociales de las aplicaciones biotecnológicas, principalmente dirigido hacia la inserción de los organismos genéticamente modificados en la agricultura. Este artículo tiene como objetivo hacer un balance de las perspectivas actuales y futuras de la aplicación de las biotecnologías vegetales en la agricultura, y de las implicaciones de su empleo para la sociedad. Se discuten los resultados más recientes obtenidos en esta área del conocimiento. Se concluye que la biotecnología vegetal ha demostrado su valor científico y aplicación práctica, aunque subsisten desigualdades en la propiedad sobre los avances y las ganancias obtenidas, las cuales tienden a mantenerse en el futuro.PALABRAS CLAVE: biotecnología vegetal, cultivo de tejidos, conservación de germoplasma, transgénesis. ABSTRACTResearch in plant biotechnology has led to the introduction of numerous scientific agriculture products, among which breeding and rapid multiplication of plant species, conservation of germplasm and transgenic plants are included. At the same time, it has remained a constant debate about the benefits and social costs of biotechnological applications, mainly directed towards the insertion of genetically modified organisms in agriculture. This article aims to take stock of current and future perspectives on application of plant biotechnologies in agriculture and their implications of use to society. The most recent results in this area of knowledge are discussed. It is concluded that plant biotechnology has demonstrated its scientific value and practical application, although there are still inequalities in the ownership of progress and profit obtained, which tend to remain in the future.

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“…Genetic manipulation and selection of desirable breeds are additional tools in traditional breeding to produce plants that are resistant to stress [9] in vitro conditions. In vitro selection of salttolerant cell cells has been reported in several varieties such as wheat [10], sugarcane [11] and fennel [12]. Safflower (Carthamus tinctorius L.) is a well quality oilseed crop [13,16].…”

Section: Introductionmentioning

confidence: 99%

Improvement of Safflower (Carthamus tinctorius L.) for Salinity Tolerance under in vitro Condition

Surbhaiyya¹,

Dhumale²,

Dudhare

et al. 2023

IJECC

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Aim: The primary aim of the present study was to screen the salt tolerant calli and optimization of in-vitro regeneration protocol from selected screen calli. Methodology: The cotyledonary leaf explants was sterilized by using 1% Bavistin, 0.1% Mercuric chloride and 70% ethanol followed by washing with distilled water. Sterilized explants were cultured on Murashige and Skoog (MS) medium supplemented with different concentration of NaCl (i.e. 0, 50mM, 100mM, 200mM, 300 mM and 400 mM) to check the salinity tolerance ability of PKV pink genotype. In vitro screening of callus was studied by morphological characters like colour and texture of callus, callus growth percentage, relative water contained, cell survivility and proline content from saline stress and unstress calli to confirm the saline tolerance pressure to regenerate the PKV Pink safflower genotype. Results: MS medium supplemented with 150 mM NaCl showed 50% survival of calli, whereas no growth was obtained in high concentration of NaCl. Moreover, biochemical assay like proline estimation was done for its confirmation from different NaCl stress and unstress calli. The proline accumulation found to be highest callus grown on MS media supplemented with 150 mM NaCl as compared to control. Also studied the morphological observation i.e. colour and texture of calli, callus growth percentage, relative water content and cell viability under different NaCl stress to select the saline tolerance pressure to regenerate the tolerance line in PKV Pink. The saline tolerance shoots failed to produce in vitro rooting on the standardized rooting medium. So, the different approach like higher auxin shock and grafting experiment were attempted to overcome the rooting problem. Higher auxin shock experiment failed but grafting approach found satisfactory to overcome the rooting in saline tolerance shoot and for development of saline tolerance line in PKV Pink safflower genotype. Conclusion: The development of abiotic stress tolerance plants found the better understanding of physiological and biochemical changes in plants under in vitro stress conditions.

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In Vitro Selection of Foeniculum vulgare for Salt Tolerance

Cited by 8 publications

References 37 publications

New basal media for half-anther culture of Anthurium andreanum Linden ex André cv. Tropical

New basal media for half-anther culture of Anthurium andreanum Linden ex André cv. Tropical

Perspectivas futuras e impacto social de las biotecnologías vegetales

Improvement of Safflower (Carthamus tinctorius L.) for Salinity Tolerance under in vitro Condition

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