In vitro selection of DNA aptamers recognizing drug-resistant ovarian cancer by cell-SELEX

He, Junqing; Wang, Junyan; Zhang, Nan; Shen, Liwei; Wang, Linlin; Xiao, Xiao; Wang, Yan; Bing, Tao; Liu, Xiangjun; Li, Songqing; Shangguan, Dihua

doi:10.1016/j.talanta.2018.10.028

Cited by 39 publications

(21 citation statements)

References 37 publications

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“…Cell-SELEX is considered to have practical applications particularly in oncology, as this approach can select aptamers specific for the cancer cell targets. Employing Cell-SELEX, aptamer-based probes have been developed for a number of cancers, including cervical cancer [27,28], ovarian cancer [29,30], liver cancer [31], prostate cancer [32], breast cancer [33,34], glioma, [35], colorectal carcinoma [36], and lung carcinoma [37]. A number of cell-SELEX variants have been developed in the last years.…”

Section: Generation Of Aptamersmentioning

confidence: 99%

Recent Advances in Aptamer Discovery and Applications

2019

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Aptamers are short, single-stranded DNA, RNA, or synthetic XNA molecules that can be developed with high affinity and specificity to interact with any desired targets. They have been widely used in facilitating discoveries in basic research, ensuring food safety and monitoring the environment. Furthermore, aptamers play promising roles as clinical diagnostics and therapeutic agents. This review provides update on the recent advances in this rapidly progressing field of research with particular emphasis on generation of aptamers and their applications in biosensing, biotechnology and medicine. The limitations and future directions of aptamers in target specific delivery and real-time detection are also discussed.

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Section: Generation Of Aptamersmentioning

confidence: 99%

Recent Advances in Aptamer Discovery and Applications

2019

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“…The binding rate of aptamer pool in the SELEX experiment library, with approximately 10 14 -10 16 random ssDNA, was applied to generate aptamers against SEA in our previous study successfully. CELL-SELEX technology, which is used for developing aptamers specific to whole cells, has been performed to screen aptamers binding to diverse tumor cells, such as prostate cancer, 10 breast cancer, 11,12 ovarian cancer cell, 13 gastric cancer, 14 and liver cancer. 15 Cell-SELEX is carried out in the native state of the target cell without any prior knowledge of the membrane protein, which not only enhances the success of screening, but also has the potential to discover new cell biomarkers.…”

Section: Discussionmentioning

confidence: 99%

“…The selection processes were performed similarly as described before 1319 . The initial ssDNA pool (2000 pmol) was dissolved in 1 ml of the selection buffer, denatured by heating at 95℃ for 5 min, and placed on ice for 10 min immediately.…”

Section: Methodsmentioning

confidence: 99%

Selection and identification of an ssDNA aptamer to NB4 cell

Zhang

Wang²,

Chen

2021

Clinical Laboratory Analysis

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“…Some of them, for various types of cancers, are described in Table 2. TTG GAG CAG CGT GGA GGA TAT GCT TTC CGA CCG TGT TCG TTT GTT ATA ACG CTG CTC C TTA AGG AGC AGC GTG GAG GAT ATC GGT GTT TAT GGT GTC TGT CTT CCT CCA GTT TCC TTC TGC GCC TT 0.30 ± 0.24 4.5 ± 1.6 [176] ALL-acute lymphoblastic leukemia; CLL-Chronic lymphocytic leukemia; AML-Acute myeloid leukemia; BL-Burkitt's lymphoma; VEGF-vascular endothelial growth factor. Z*-gemcitabine is a first-line chemotherapy agent for the treatment of pancreatic cancer.…”

Section: Aptamers For Cancer Markersmentioning

confidence: 99%

Polymer Nanoparticles and Nanomotors Modified by DNA/RNA Aptamers and Antibodies in Targeted Therapy of Cancer

2021

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Polymer nanoparticles and nano/micromotors are novel nanostructures that are of increased interest especially in the diagnosis and therapy of cancer. These structures are modified by antibodies or nucleic acid aptamers and can recognize the cancer markers at the membrane of the cancer cells or in the intracellular side. They can serve as a cargo for targeted transport of drugs or nucleic acids in chemo- immuno- or gene therapy. The various mechanisms, such as enzyme, ultrasound, magnetic, electrical, or light, served as a driving force for nano/micromotors, allowing their transport into the cells. This review is focused on the recent achievements in the development of polymer nanoparticles and nano/micromotors modified by antibodies and nucleic acid aptamers. The methods of preparation of polymer nanoparticles, their structure and properties are provided together with those for synthesis and the application of nano/micromotors. The various mechanisms of the driving of nano/micromotors such as chemical, light, ultrasound, electric and magnetic fields are explained. The targeting drug delivery is based on the modification of nanostructures by receptors such as nucleic acid aptamers and antibodies. Special focus is therefore on the method of selection aptamers for recognition cancer markers as well as on the comparison of the properties of nucleic acid aptamers and antibodies. The methods of immobilization of aptamers at the nanoparticles and nano/micromotors are provided. Examples of applications of polymer nanoparticles and nano/micromotors in targeted delivery and in controlled drug release are presented. The future perspectives of biomimetic nanostructures in personalized nanomedicine are also discussed.

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In vitro selection of DNA aptamers recognizing drug-resistant ovarian cancer by cell-SELEX

Cited by 39 publications

References 37 publications

Recent Advances in Aptamer Discovery and Applications

Recent Advances in Aptamer Discovery and Applications

Selection and identification of an ssDNA aptamer to NB4 cell

Polymer Nanoparticles and Nanomotors Modified by DNA/RNA Aptamers and Antibodies in Targeted Therapy of Cancer

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