In vitro seed maturation in Brassica rapa L.: Relationship of silique atmosphere to storage reserve deposition

Musgrave, Mary E.; Allen, Joan; Blasiak, J.; Tuominen, Lindsey K.; Kuang, Anxiu

doi:10.1016/j.envexpbot.2007.09.004

Cited by 8 publications

(12 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In particular, the progression of events during the maturation phase of the seed has been portrayed as a shift from starch to lipid storage (e.g. Eastmond & Rawsthorne, 2000;Schwender et al, 2003;Vigeolas et al, 2003Vigeolas et al, , 2004Lin et al, 2006;Musgrave et al, 2008). Our results show that these assumptions are oversimplifications.…”

Section: Spatial and Temporal Patterns Of Starch Accumulation In The mentioning

confidence: 80%

Starch turnover in developing oilseed embryos

et al. 2010

View full text Add to dashboard Cite

Summary• Starch accumulates early during embryo development in Arabidopsis and oilseed rape, then disappears during oil accumulation. Little is known about the nature and importance of starch metabolism in oilseed embryos.• Histochemical and quantitative measures of starch location and content were made on developing seeds and embryos from wild-type Arabidopsis plants, and from mutants lacking enzymes of starch synthesis and degradation with established roles in leaf starch turnover. Feeding experiments with [14 C]sucrose were used to measure the rate of starch synthesis in oilseed rape embryos within intact siliques.• The patterns of starch turnover in the developing embryo are spatially and temporally complex. Accumulation is associated with zones of cell division. Study of mutant plants reveals a major role in starch turnover for glucan, water dikinase (absent from the sex1 mutant) and isoforms of beta-amylase (absent from various bam mutants). Starch is synthesized throughout the period of its accumulation and loss in embryos within intact siliques of oilseed rape.• We suggest that starch turnover is functionally linked to cell division and differentiation rather than to developmental or storage functions specific to embryos. The pathways of embryo starch metabolism are similar in several respects to those in Arabidopsis leaves.

show abstract

Section: Spatial and Temporal Patterns Of Starch Accumulation In The mentioning

confidence: 80%

Starch turnover in developing oilseed embryos

et al. 2010

View full text Add to dashboard Cite

show abstract

“…The ability to manipulate resource partitioning and assimilate transport into the seeds and pods could help maximize overall yield (Wardlaw, 1990). Studies using detached pods have shown that the photosynthetic tissues of the pod wall are capable of generating 60% of seed assimilates (reviewed in Diepenbrock, 2000), although it must be noted that in vitro pod growth results in a decrease in internal pod O 2 concentrations compared with growth in vivo , which can alter the amount of storage compound within a seed (Musgrave et al. , 2008).…”

Section: Resource Transport Into the Seeds Via The Podmentioning

confidence: 99%

The role of the pod in seed development: strategies for manipulating yield

2011

View full text Add to dashboard Cite

SummaryPods play a key role in encapsulating the developing seeds and protecting them from pests and pathogens. In addition to this protective function, it has been shown that the photosynthetically active pod wall contributes assimilates and nutrients to fuel seed growth. Recent work has revealed that signals originating from the pod may also act to coordinate grain filling and regulate the reallocation of reserves from damaged seeds to those that have retained viability. In this review we consider the evidence that pods can regulate seed growth and maturation, particularly in members of the Brassicaceae family, and explore how the timing and duration of pod development might be manipulated to enhance either the quantity of crop yield or its nutritional properties.

show abstract

“…This includes the demonstration of hypoxic, ATPlimited conditions during seed fill (Vigeolas et al, 2003;Musgrave et al, 2008;Borisjuk and Rolletschek, 2009), the high expression of SUS, UGPase, and PFP genes and enzymes during the maturation phase of seed filling (Baud and Graham, 2006;Troncoso-Ponce et al, 2011), and the fact that SUS and PFP genes respond to WRI1 expression (Focks and Benning, 1998;Ruuska et al, 2002). Thus, the use of PPi appears to be part of an ATP-saving, SUS-initiated pathway of cytosolic Suc catabolism characteristic of the maturation phase of seed development with highest rates of seed storage compound accumulation.…”

Section: Role Of Pyrophosphatases In Seed Fillingmentioning

confidence: 99%

Oil and Protein Accumulation in Developing Seeds Is Influenced by the Expression of a Cytosolic Pyrophosphatase in Arabidopsis

et al. 2012

View full text Add to dashboard Cite

This study describes a dominant low-seed-oil mutant (lo15571) of Arabidopsis (Arabidopsis thaliana) generated by enhancer tagging. Compositional analysis of developing siliques and mature seeds indicated reduced conversion of photoassimilates to oil. Immunoblot analysis revealed increased levels of At1g01050 protein in developing siliques of lo15571. At1g01050 encodes a soluble, cytosolic pyrophosphatase and is one of five closely related genes that share predicted cytosolic localization and at least 70% amino acid sequence identity. Expression of At1g01050 using a seed-preferred promoter recreated most features of the lo15571 seed phenotype, including low seed oil content and increased levels of transient starch and soluble sugars in developing siliques. Seed-preferred RNA interference-mediated silencing of At1g01050 and At3g53620, a second cytosolic pyrophosphatase gene that shows expression during seed filling, led to a heritable oil increase of 1% to 4%, mostly at the expense of seed storage protein. These results are consistent with a scenario in which the rate of mobilization of sucrose, for precursor supply of seed storage lipid biosynthesis by cytosolic glycolysis, is strongly influenced by the expression of endogenous pyrophosphatase enzymes. This emphasizes the central role of pyrophosphate-dependent reactions supporting cytosolic glycolysis during seed maturation when ATP supply is low, presumably due to hypoxic conditions. This route is the major route providing precursors for seed oil biosynthesis. ATP-dependent reactions at the entry point of glycolysis in the cytosol or plastid cannot fully compensate for the loss of oil content observed in transgenic events with increased expression of cytosolic pyrophosphatase enzyme in the cytosol. These findings shed new light on the dynamic properties of cytosolic pyrophosphate pools in developing seed and their influence on carbon partitioning during seed filling. Finally, our work uniquely demonstrates that genes encoding cytosolic pyrophosphatase enzymes provide novel targets to improve seed composition for plant biotechnology applications.

show abstract

In vitro seed maturation in Brassica rapa L.: Relationship of silique atmosphere to storage reserve deposition

Cited by 8 publications

References 30 publications

Starch turnover in developing oilseed embryos

Starch turnover in developing oilseed embryos

The role of the pod in seed development: strategies for manipulating yield

Oil and Protein Accumulation in Developing Seeds Is Influenced by the Expression of a Cytosolic Pyrophosphatase in Arabidopsis

Contact Info

Product

Resources

About