In-vitro secretion of ACTH in Nelson's syndrome

Lüdecke, D. K.; Schabet, Martin; Westphal, Manfred; Saeger, Wolfgang

doi:10.1007/bf01405438

Cited by 5 publications

(1 citation statement)

References 26 publications

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“…Electron microscopy permitted a much better morphological analysis and consequently more accurate morpho-functional classification of pituitary microadenomas 5,9,11,[14][15][16][17]19 This study technique is necessary, especially to assert the diagnosis of oncocytoma lo, 1~, la, e0 and to evaluate the morphological degree of secretory activity of microadenomas 11, 17, ,9, 21 …”

Section: O N C L U S I O N Smentioning

confidence: 99%

Pituitary microadenomas: Surgical results and morphological findings

Scanarini¹,

Mingrino²,

Boscaro³

et al. 1983

Acta neurochir

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Section: O N C L U S I O N Smentioning

confidence: 99%

Pituitary microadenomas: Surgical results and morphological findings

Scanarini¹,

Mingrino²,

Boscaro³

et al. 1983

Acta neurochir

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Long-term culture of human corticotropin-secreting adenomas on extracellular matrix and evaluation of serum-free conditions

1986

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Tissues from 12 human corticotropin-secreting adenomas, obtained during surgery for Cushing's disease (CD, ten cases) or Nelson's Syndrome (NS, two cases), were exclusively mechanically dispersed. Single cells and cell aggregates were plated on extracellular matrix derived from bovine corneal endothelia. Functional responses to physiological stimuli were analyzed by measuring human beta-endorphin (beta h-EP) immunoreactivity (IR) by radioimmunoassay in the culture medium. All adenomas responded with stimulated secretory activity to arginine vasopressin (AVP), corticotropin-releasing factor (CRF), or both. Cortisol higher than 10(-8) M suppressed basal secretion and CRF- or AVP-stimulated beta h-EP-IR secretion. There was no consistent difference in response of the cells when cultured in medium containing 10% fetal calf serum (FCS) or in serum-free conditions. A change of cells from serum to serum-free conditions usually resulted in 10%-50% reduction in the basal secretion level that remained stable for at least 2 weeks and, in one case (NS), 10 weeks. In cells maintained in medium supplemented with 5% serum obtained from the respective patients 40 min after adenoma removal, basal secretion was suppressed to 60% of the baseline level in a 10% FCS control. Long-term incubation with CRF (10(-9) M) showed sustained stimulation of hormone secretion. No remarkable cell proliferation was observed under basal conditions or during long-term, low-dose incubation with cholera toxin (10(-12) M) in two cases (CD), or CRF (10(-9) M) in two cases (NS, CD). Parallel beta-EP-IR and adrenocorticotropin secretion was verified in selected cases.

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Long-term culture of human corticotropin-secreting adenomas on extracellular matrix and evaluation of serum-free conditions

1986

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Tissues from 12 human corticotropin-secreting adenomas, obtained during transsphenoidal surgery for Cushing's disease (CD, ten cases) or Nelson's syndrome (NS, two cases), were mechanically dispersed. The resulting single cells and cell aggregates were plated on extracellular matrix derived from bovine corneal endothelia. CD and NS cells showed distinct morphological differences initially, CD cells being much more spherical than the flattened NS cells. By 10 days at the latest after plating, however, CD and NS cells were indistinguishable morphologically. Cultured cells from both entities responded with rounding to cortisol (hydrocortisone, 10(-6) M) within 4-6 h. Synthetic ovine corticotropin-releasing factor (10(-8) M) produced flattening and extension of cytoplasmic processes after as early as 2 h.

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In-vitro secretion of ACTH in Nelson's syndrome

Cited by 5 publications

References 26 publications

Pituitary microadenomas: Surgical results and morphological findings

Pituitary microadenomas: Surgical results and morphological findings

Long-term culture of human corticotropin-secreting adenomas on extracellular matrix and evaluation of serum-free conditions

Long-term culture of human corticotropin-secreting adenomas on extracellular matrix and evaluation of serum-free conditions

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