In Vitro Secondary Metabolite Production Through Fungal Elicitation: An Approach for Sustainability

Shasmita,; Singh, Nihar Ranjan; Rath, Sakti Kanta; Behera, Shashikanta; Naik, Soumendra K.

doi:10.1007/978-981-10-8666-3_9

Cited by 11 publications

(5 citation statements)

References 134 publications

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“…The endophytic fungal elicitor has also been shown to increase the expression of specific genes in medicinal plants, which can result in enhanced accumulation of bioactive molecules by the activation of a series of specific secondary metabolic pathways (Zhai et al, 2017). Various strategies using fungal elicitors for enhancing the production of secondary metabolites have been briefly described by Shasmita et al (2018) and Baldi et al (2009). The fungal elicitor Aspergillus flavus enhanced the total indole alkaloid production of Catharanthusroseus cambial meristematic cell cultures.…”

Section: Discussionmentioning

confidence: 99%

Effect of biotic elicitors on polyphenol production in Cayratia trifolia cell suspension cultures analyzed by HPLC

Arora¹,

Joshi²,

Kanthaliya³

et al. 2020

bta

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Cayratia trifolia Linn. Domin Syn. Vitis trifolia (family: Vitaceae) contains polyphenols as major bioactive compounds. Plant polyphenolics, such as stilbenes, possess a broad spectrum of pharmacological and therapeutic effects, including antioxidative, anticancerous, antiatherosclerotic, cardioprotective, hepatoprotective, and neuroprotective effects. Elicitation is one of the important strategies to enhance secondary metabolite production in cell suspension cultures. In the present study, the effect of two biotic elicitors, namely one fungal (Fusarium sp., and Helminthosporium sp.) extract and another higher plant-originated gum (Anogeissus latifolia), was evaluated for enhanced production of stilbenes in cell suspension culture of C. trifolia. For the fungal elicitor, the cell suspension cultures of C. trifolia were treated with varying concentrations of Fusarium extract (FE) and Helminthosporium extract (HE) on the 7 th day of culture. Increasing concentration of FE in the medium resulted in slightly decreased cell growth but increased the production of stilbenes, with the optimal concentration at 1000 mg/l. This increase in the yield of stilbenes was-5 times (9.30 mg/l) higher than the control value. HE was less effective than FE in increasing stilbene production at optimal concentrations. The addition of 50 mg/l A. latifolia plant gum sieved through a 150 mesh (0.1 mm) to C. trifolia cell culture enhanced stilbene accumulation and yield by 8-to 9-fold over the control value.

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Section: Discussionmentioning

confidence: 99%

Effect of biotic elicitors on polyphenol production in Cayratia trifolia cell suspension cultures analyzed by HPLC

Arora¹,

Joshi²,

Kanthaliya³

et al. 2020

bta

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“…Dependence on medicinal plants for production of significant bioactive compounds for the development of novel drugs has a few drawbacks, including variation in secondary metabolites produced due to seasonal, spatial, and environmental conditions ( Singh et al., 2018 ; Van Wyk and Prinsloo, 2020 ). Additionally, some medicinal plants have long generation time to reach maturity while some are strictly seasonal and thus unavailable during the off-season ( Verma and Shukla, 2015 ; Isah, 2019 ).…”

Section: Endophytes As An Important Source Of Bioactive Compoundsmentioning

confidence: 99%

“…Mass production of bioactive compounds from medicinal plants often requires a high volume of biomass. The quality and quantity of naturally synthesized bioactive compounds fluctuate with varying environmental growth conditions, compounded by the global climate change ( Venugopalan and Srivastava, 2015 ; Singh et al., 2018 ). Thus, there is a growing need for sustainable alternative templates or sources for efficient production of drugs to meet the ever-increasing demands ( Asmita and Sashirekha, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Contribution of endophytes towards improving plant bioactive metabolites: a rescue option against red-taping of medicinal plants

Tsipinana,

Husseiny,

Alayande

et al. 2023

Front. Plant Sci.

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Medicinal plants remain a valuable source for natural drug bioprospecting owing to their multi-target spectrum. However, their use as raw materials for novel drug synthesis has been greatly limited by unsustainable harvesting leading to decimation of their wild populations coupled with inherent low concentrations of constituent secondary metabolites per unit mass. Thus, adding value to the medicinal plants research dynamics calls for adequate attention. In light of this, medicinal plants harbour endophytes which are believed to be contributing towards the host plant survival and bioactive metabolites through series of physiological interference. Stimulating secondary metabolite production in medicinal plants by using endophytes as plant growth regulators has been demonstrated to be one of the most effective methods for increasing metabolite syntheses. Use of endophytes as plant growth promotors could help to ensure continuous supply of medicinal plants, and mitigate issues with fear of extinction. Endophytes minimize heavy metal toxicity in medicinal plants. It has been hypothesized that when medicinal plants are exposed to harsh conditions, associated endophytes are the primary signalling channels that induce defensive reactions. Endophytes go through different biochemical processes which lead to activation of defence mechanisms in the host plants. Thus, through signal transduction pathways, endophytic microorganisms influence genes involved in the generation of secondary metabolites by plant cells. Additionally, elucidating the role of gene clusters in production of secondary metabolites could expose factors associated with low secondary metabolites by medicinal plants. Promising endophyte strains can be manipulated for enhanced production of metabolites, hence, better probability of novel bioactive metabolites through strain improvement, mutagenesis, co-cultivation, and media adjustment.

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“…Birat et al [4] recently reported that the fungus Nigrospora zimmermanii, which is present within the leaves of Catharanthus roseus, also produced vincristine successfully. The strategies frequently used for enriching the levels of alkaloids are the optimization of media, plant growth regulators, and cultural practices; the culture of high-yielding cell lines; the use of precursors; the incorporation of elicitors; and improving the expression of the regulatory enzymes of metabolic pathways [5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Fungal Elicitation Enhances Vincristine and Vinblastine Yield in the Embryogenic Tissues of Catharanthus roseus

Tonk,

Mujib,

Maqsood

et al. 2023

Plants

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Fungal elicitation could improve the secondary metabolite contents of in vitro cultures. Herein, we report the effect of Fusarium oxysporum on vinblastine and vincristine alkaloid yields in Catharanthus roseus embryos. The study revealed increased yields of vinblastine and vincristine in Catharanthus tissues. Different concentrations, i.e., 0.05% (T1), 0.15% (T2), 0.25% (T3), and 0.35% (T4), of an F. oxysporum extract were applied to a solid MS medium in addition to a control (T0). Embryogenic calli were formed from the hypocotyl explants of germinating seedlings, and the tissues were exposed to Fusarium extract elicitation. The administration of the F. oxysporum extract improved the growth of the callus biomass, which later differentiated into embryos, and the maximum induction of somatic embryos was noted T2 concentration (102.69/callus mass). A biochemical analysis revealed extra accumulations of sugar, protein, and proline in the fungus-elicitated cultivating tissues. The somatic embryos germinated into plantlets on full-strength MS medium supplemented with 2.24 µM of BA. The germination rate of the embryos and the shoot and root lengths of the embryos were high at low doses of the Fusarium treatment. The yields of vinblastine and vincristine were measured in different treated tissues via high-pressure thin-layer chromatography (HPTLC). The yield of vinblastine was high in mature (45-day old) embryos (1.229 µg g−1 dry weight), which were further enriched (1.267 µg g−1 dry weight) via the F. oxysporum-elicitated treatment, especially at the T2 concentration. Compared to vinblastine, the vincristine content was low, with a maximum of 0.307 µg g−1 dry weight following the addition of the F. oxysporum treatment. The highest and increased yields of vinblastine and vincristine, 7.88 and 15.50%, were noted in F. oxysporum-amended tissues. The maturated and germinating somatic embryos had high levels of SOD activity, and upon the addition of the fungal extracts, the enzyme’s activity was further elevated, indicating that the tissues experienced cellular stress which yielded increased levels of vinblastine and vincristine following the T2/T1 treatments. The improvement in the yields of these alkaloids could augment cancer healthcare treatments, making them easy, accessible, and inexpensive.

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In Vitro Secondary Metabolite Production Through Fungal Elicitation: An Approach for Sustainability

Cited by 11 publications

References 134 publications

Effect of biotic elicitors on polyphenol production in Cayratia trifolia cell suspension cultures analyzed by HPLC

Effect of biotic elicitors on polyphenol production in Cayratia trifolia cell suspension cultures analyzed by HPLC

Contribution of endophytes towards improving plant bioactive metabolites: a rescue option against red-taping of medicinal plants

Fungal Elicitation Enhances Vincristine and Vinblastine Yield in the Embryogenic Tissues of Catharanthus roseus

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