In vitro screening of two Nigerian medicinal plants (Vernonia amygdalina and Annona senegalensis) for anthelmintic activity

Alawa, C. B. I.; Adamu, A. M.; Gefu, JO; Ajanusi, O.J.; Abdu, Paul Ayuba; Chiezey, N. P.; Alawa, Judith Nkiruka; Bowman, Dwight D.

doi:10.1016/s0304-4017(03)00040-2

Cited by 169 publications

(117 citation statements)

References 21 publications

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“…Among various kinds of in vitro methods, the egg hatch assays (Coles et al, 1992;Ketzis et al, 2002;Pessoa et al, 2002;Alawa et al, 2003;Hounzangbe-Adote et al, 2005) and the larval motility tests (Rabel et al, 1994;Lorimer et al, 1996;Khunkitti et al, 2000;HounzangbeAdote et al, 2005) are commonly used to test medicinal plants against nematodes.…”

Section: Introductionmentioning

confidence: 99%

In VitroAnthelmintic Effects of Medicinal Plants Used in Czech Republic

Urban

Kokoška

Langrová

et al. 2008

Pharmaceutical Biology

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The ethanol extracts of 16 Czech medicinal plants, namely, . sativum, A. absinthium, C. carvi, D. carota, and J. regia possessed the strongest anthelmintic effect on the embryonating eggs at all concentrations tested (62.5, 125, 250, 500, 1000, 2000 µg/mL). The best results, showing a higher effect against the infective third-stage larvae in comparison with synthetic anthelmintic Zentel (albendazole), have been obtained for A. sativum, A. absinthium, C. carvi, C. regalis, I. helenium, J. regia, S. hortensis, and V. officinalis.

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Section: Introductionmentioning

confidence: 99%

In VitroAnthelmintic Effects of Medicinal Plants Used in Czech Republic

Urban

Kokoška

Langrová

et al. 2008

Pharmaceutical Biology

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“…According to Leão (2007), such compounds are called secondary metabolites and are related to the chemical plant defense, guaranteeing their survival in their natural habitat. Furthermore, those compounds present a wide diversity and their presence in a given solution will depend on the extraction process and extraction solution used (Alawa et al, 2003).…”

Section: Resultsmentioning

confidence: 99%

“…However, all the concentrations showed inhibition above 93.0%, while in control groups there was no larvae inhibition during the first 24 hours Among the extraction methods evaluated, EAM presented as an efficient and affordable alternative to be used in helminthes control. The preparation of medicinal species in water is a commonly used practice in folk medicine and its use as a natural vermifuge is becoming more popular as an alternative method, sustainable and adaptable for rural communities (Alawa et al, 2003;Fonseca et al, 2005;Bizimenyera et al, 2006). However, aiming to obtain a formulation for large-scale industrial production, lyophilized extract is presented as a more acceptable formulation because it provides greater physical, chemical and microbial stability, and facilitates homogenization and standardization of the active ingredients (Runha et al, 2001;Chaicouski et al, 2014).…”

Section: Resultsmentioning

confidence: 99%

Anthelmintic potential and in vivo acute toxicity study of Piptadenia gonoachanta leaf aqueous extract

2017

J App Pharm Sci

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Piptadenia gonoacantha (Leguminosae) is an arboreal species found in southern and southeastern Brazil. Several compounds have been identified in their leaves, such as anthraquinones, phenolics, tannins, saponins, coumarins and flavonoids. The presence of these metabolites is associated with antioxidant, anti-inflammatory, antibiotic and anthelmintic properties. This study evaluated the phytochemical profile of in vitro anthelmintic activity and in vivo toxicity of the aqueous extract of P. gonoacantha leaves. The extracts were distinctly evaluated by extraction method: maceration (EAM), ultrasonication (EAU) and lyophilization (EAL). Anthelmintic activity analysis was carried out for all preparations at 25, 50 and 100 mg.mL -1 against Panagrellus sp. in an in vitro assay. In vivo toxicity was assessed by biochemical, hematological and histological evaluation of Balb/C mice treated orally with EAU (100, 200 and 400 mg.Kg -1 ) for 10 consecutive days. Phytochemical analysis showed the presence of tannins, flavonoids, coumarins, saponins, glycosides, phenolic compounds and anthraquinone. Anthelmintic potential showed significant results, with inhibition potential ranging from 93.93% and 99.88% among the different concentrations and preparations tested. In absolute values, the EAPG at 50 mg / mL showed a higher percentage of inhibition, reaching the following concentrations: 25, 50 and 100 mg / mL of preparation I (EAM), 25 and 100 mg / mL of preparation II (EAU) and 25 , 50 and 100 mg / mL of preparation III (EAL), had percentage of inhibition of 99.39%, 96.15%, 96.57%, 96.45%, 95.01%, 99.74% and 99% 44% respectively. EAU has proved to be safe for in vivo use because it showed low toxicity, enabling it to future pre-clinical and clinical studies.

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“…The length of time that Sarcoptes scabiei can survive off the host depends on environmental conditions but may be between 2 and 3 weeks [11]. The ideal approach for diagnosis of skin diseases in general is a logical progression from history to an overall clinical examination, to a detailed examination of the skin, and finally to confirmatory testing or diagnosis by response to treatment [22,23].…”

Section: Journal Ofmentioning

confidence: 99%

Evaluation of Acaricidal Effect of Ethnoveteinary Medicinal Plant by in vivo and in vitro against Sarcoptes scabiei var. caprae of Infected Goats in North Shoa, Oromia Regional State, Ethiopia

Kebede¹,

Negese²

2017

J Tradi Med Clin Natur

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This study was conducted to determine parasiticidal efficacy of seven ethnomedicinal plants against Sarcoptes scabiei var. caprae of goats using in vivo and in vitro techniques. In vitro techniques for evaluation of efficacy of medicinal plants essential oils and fixed oils of seven medicinal plant extracts diluted at different concentrations (essential oils from 2.5% to 0.15625% and fixed oils from 160 mg/ml to 5 mg/ml) were added to petridishes containing adult stage of Sarcoptes scabiei var. caprae. After 3 h of contact, all concentrations of essential oil of Eucalyptus globulus and Cymbopogon citractus showed a good in vitro acaricidal efficacy as compared with the non-treated controls (p<0.05). However, Nicotiana tobacum fixed oil had significant (P<0.05) effect at a concentration of 160 mg/ml and 80 mg/ml. Pyrethrum cineraria folium fixed oil showed lower acaricidal efficacy (P<0.05) in all the concentrations of the extract as compared to the reference drugs. In vivo techniques undertaken for evaluation of efficacy essential oil of Eucalyptus globulus and Cymbopogon citractus at a concentration of 0.625% in 2% Tween 80 on two groups of (six animals each) Sarcoptes scabiei var. caprae infested goats were topically treated two times at 14 days interval and its compared with non-treated and treated (diazinone and ivermectin) controls of six goats in each group. The infected goats treated with the essential oils were cured completely. Statistically insignificant (p>0.05) difference was never observed in mite count, Mean Recovery Response (MRR) and degree of Skin Lesion Quality (SLQ) between goats treated with plant extracts and those treated with diazinone and ivermectin. Therefore, Eucalyptus globulus and Cymbopogon citractus extracts should be licensed for the treatment of Sarcoptes scabiei var. caprae.

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In vitro screening of two Nigerian medicinal plants (Vernonia amygdalina and Annona senegalensis) for anthelmintic activity

Cited by 169 publications

References 21 publications

In VitroAnthelmintic Effects of Medicinal Plants Used in Czech Republic

In VitroAnthelmintic Effects of Medicinal Plants Used in Czech Republic

Anthelmintic potential and in vivo acute toxicity study of Piptadenia gonoachanta leaf aqueous extract

Evaluation of Acaricidal Effect of Ethnoveteinary Medicinal Plant by in vivo and in vitro against Sarcoptes scabiei var. caprae of Infected Goats in North Shoa, Oromia Regional State, Ethiopia

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