In Vitro Salivary Protein Adsorption Profile on Titanium and Ceramic Surfaces and the Corresponding Putative Immunological Implications

Wei, Chenxuan; Burrow, Michael F.; Botelho, MG; Lam, Henry; Leung, W. Keung

doi:10.3390/ijms21093083

Cited by 10 publications

(13 citation statements)

References 65 publications

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“…Being quite simple, binary protein solutions are still not very much representative of actual biological fluids. Some researchers moved further on in complexity of systems by investigating through proteomic analysis the exact composition of protein layers adsorbed on several titanium surfaces from real and whole biological fluids such as plasma [ 221 , 228 , 229 ] or saliva [ 230 , 231 , 232 ]. Among the thousands of proteins present in human plasma, the most adsorbed was FN, followed by albumin, alipoprotein, and fibrinogen [ 221 ].…”

Section: Protein Co-adsorption and Competition For The Surfacementioning

confidence: 99%

“…Among the thousands of proteins present in human plasma, the most adsorbed was FN, followed by albumin, alipoprotein, and fibrinogen [ 221 ]. From saliva, which contains about 750 different proteins, less than half of them were found on titanium [ 232 ], mainly amylase and lysozyme [ 230 ]. The effect of surface modification on the protein pellicle composition was also evaluated.…”

Section: Protein Co-adsorption and Competition For The Surfacementioning

confidence: 99%

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Titanium and Protein Adsorption: An Overview of Mechanisms and Effects of Surface Features

2021

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Titanium and its alloys, specially Ti6Al4V, are among the most employed materials in orthopedic and dental implants. Cells response and osseointegration of implant devices are strongly dependent on the body–biomaterial interface zone. This interface is mainly defined by proteins: They adsorb immediately after implantation from blood and biological fluids, forming a layer on implant surfaces. Therefore, it is of utmost importance to understand which features of biomaterials surfaces influence formation of the protein layer and how to guide it. In this paper, relevant literature of the last 15 years about protein adsorption on titanium-based materials is reviewed. How the surface characteristics affect protein adsorption is investigated, aiming to provide an as comprehensive a picture as possible of adsorption mechanisms and type of chemical bonding with the surface, as well as of the characterization techniques effectively applied to model and real implant surfaces. Surface free energy, charge, microroughness, and hydroxylation degree have been found to be the main surface parameters to affect the amount of adsorbed proteins. On the other hand, the conformation of adsorbed proteins is mainly dictated by the protein structure, surface topography at the nano-scale, and exposed functional groups. Protein adsorption on titanium surfaces still needs further clarification, in particular concerning adsorption from complex protein solutions. In addition, characterization techniques to investigate and compare the different aspects of protein adsorption on different surfaces (in terms of roughness and chemistry) shall be developed.

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Section: Protein Co-adsorption and Competition For The Surfacementioning

confidence: 99%

Section: Protein Co-adsorption and Competition For The Surfacementioning

confidence: 99%

Titanium and Protein Adsorption: An Overview of Mechanisms and Effects of Surface Features

2021

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“…In another in vitro study, the composition of the salivary pellicle formed on smooth Ti substrates was investigated by MS; the findings showed that 10 salivary proteins had an affinity for the Ti surfaces, 16 among the identified proteins, they found; prolactin‐inducible protein and alpha‐amylase 1, which also were found adsorbed on the Ti substrates tested in the present study. Most recently, 495 different proteins were detected, under in vitro conditions, in the salivary pellicle formed on Ti surfaces exposed to stimulated human saliva, 31 while in another in vitro study, 369 different proteins were detected on the salivary pellicle formed on Ti surfaces 18 . In the present study, the protein extraction protocol and the proteomic analysis allowed the identification of 301 proteins adsorbed on the Ti implant surfaces.…”

Section: Discussionmentioning

confidence: 55%

“…In contrast, less is known regarding the composition of the salivary pellicle formed on marked dental implant surfaces. in vitro studies have frequently reported as principal constituents of the salivary pellicle formed on Ti surfaces proteins like serum albumin, salivary amylase, prolactin‐inducible protein, and cystatin‐SA 16‐18 . However, the salivary pellicle's protein content formed under in situ conditions on Ti implant surfaces has not been thoroughly explored.…”

Section: Introductionmentioning

confidence: 99%

Roughness and wettability of titanium implant surfaces modify the salivary pellicle composition

et al. 2020

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This study reports the differences in the protein composition of salivary pellicles formed under in situ conditions on two Titanium (Ti) surfaces, with different roughness and wettability. Smooth pretreatment Ti surfaces (Ti‐PT) with an average roughness (Ra) of 0.45 μm and a water contact angle (WCA) of 92.4°, as well as a more rough sandblasted, large grit, acid‐etched treatment Ti surfaces (Ti‐SLA) with a Ra of 3.3 μm and WCA of 131.8°, were tested. The salivary pellicles were quantitatively analyzed by bicinchoninic acid assays, and the protein identification was performed by Nano‐LC–MS/MS (nano mass spectrometry). Protein levels of 2.5, and 9.1 μg/ml were quantified from the detached salivary pellicle formed on the Ti‐PT and Ti‐SLA surfaces, respectively. Using Nano‐LC–MS/MS, a total of 597 proteins were identified on all the substrates tested; 43 proteins were identified only on the Ti‐PT, and 226 proteins were adsorbed solely on the Ti‐SLA substrates. The physicochemical characteristics of the Ti implant surfaces modified the amount and the identity of the salivary proteome of the pellicles formed, confirming the high selectivity of the protein pellicle formed on a surface once is exposed in the oral cavity.

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“…Although the peri-implant microbiome is unique, these biofilms establish on the surface of implanted materials in a similar manner to that on the natural dentition. A dental pellicle proteome is formed directly on the implant surface [14,15]. Early colonizing microorganisms then attach to the pellicle via van der Waals forces, electrostatic charges, and specific adhesive proteins [16].…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Prosthetic Surfaces in the Oral Cavity—A Perspective on Creative Approaches

et al. 2020

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Replacement of missing teeth is an essential component of comprehensive dental care for patients suffering of edentulism. A popular option is implant-supported restorations. However, implant surfaces can become colonized with polymicrobial biofilms containing Candida species that may compromise peri-implant health. To prevent this, implant components may be treated with a variety of coatings to create surfaces that either repel the attachment of viable microorganisms or kill microorganisms on contact. These coatings may consist of nanoparticles of pure elements (more commonly silver, copper, and zinc), sanitizing agents and disinfectants (quaternary ammonium ions and chlorhexidine), antibiotics (cefalotin, vancomycin, and gentamicin), or antimicrobial peptides (AMPs). AMPs in bioactive coatings have a number of advantages. They elicit a protective action against pathogens, inhibit the formation of biofilms, are less toxic to host tissues, and do not prompt inflammatory responses. Furthermore, many of these coatings may involve unique delivery systems to direct their antimicrobial capacity against pathogens, but not commensals. Coatings may also contain multiple antimicrobial substances to widen antimicrobial activity across multiple microbial species. Here, we compiled relevant information about a variety of creative approaches used to generate antimicrobial prosthetic surfaces in the oral cavity with the purpose of facilitating implant integration and peri-implant tissue health.

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In Vitro Salivary Protein Adsorption Profile on Titanium and Ceramic Surfaces and the Corresponding Putative Immunological Implications

Cited by 10 publications

References 65 publications

Titanium and Protein Adsorption: An Overview of Mechanisms and Effects of Surface Features

Titanium and Protein Adsorption: An Overview of Mechanisms and Effects of Surface Features

Roughness and wettability of titanium implant surfaces modify the salivary pellicle composition

Antimicrobial Prosthetic Surfaces in the Oral Cavity—A Perspective on Creative Approaches

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