In vitro rumen fermentation characteristics and microbes of thin tail sheep given sweet potato (Ipomoea batatas L) biomass

Abstract

ABSTRAKSudarman A, Hayashida M, Miralestari M. 2016. Karakteristik rumen fermentasi secara in vitro dan mikroba domba ekor tipis yang diberikan biomassa ubi jalar. JITV 21(2): 83-87. DOI: http://dx.doi.org/10. 14334/jitv.v21i2.1355 Tanaman ubi jalar (Ipomoea batatas L) diproduksi di Indonesia dalam jumlah besar. Daun dan batang ubi jalar dapat digunakan sebagai sumber protein dan umbinya dapat digunakan sebagai sumber energi. Penelitian ini bertujuan untuk mengkaji pengaruh pemberian biomasa ubi jalar pada karakteristik fermentasi rumen, kecernaan bahan kering dan bahan organic, serta populasi mikroba rumen domba ekor tipis. Penelitian ini menggunakan rancangan acak kelompok dengan menerapkan empat perlakuan pakan, yaitu, T0 (100% rumput gajah), T1 (70% dari rumput gajah + 30% konsentrat), T2 (50% dari rumput gajah + 50% daun ubi jalar), dan T3 (70% daun ubi jalar + 30% umbi ubi jalar) dengan tiga ulangan. Sampel cairan rumen diambil dengan metode stomach tube dengan menggunakan pompa vakum. Hasil penelitian menunjukkan bahwa perlakuan T3 memiliki kecernaan bahan kering dan bahan organik, konsentrasi NH3 dan VFA, dan populasi bakteri rumen lebih tinggi (P<0,05) daripada perlakuan T0 dan T1 tetapi tidak berbeda dengan perlakuan T2. Populasi protozoa dan pH rumen tidak berbeda antar perlakuan. Dapat disimpulkan bahwa penggunaan biomassa ubi jalar dapat meningkatkan kualitas fermentasi rumen domba. Sweet potato plant (Ipomoea batatas L) is produced in Indonesia in large quantities. Sweet potato leaves and stems can be used as a source of protein and the tuber can be used as an energy source. This study was aimed to analyze the effect of feeding of sweet potato biomass on the rumen fermentation characteristics, digestibility of dry matter and organic matter as well as rumen microbial population of thin tail sheep. This study used a randomized block design by applying four feed treatments, i.e., T0 (100% Napier grass), T1 (70% of Napier grass + 30% concentrate), T2 (50% of Napier grass + 50% sweet potato leaves), and T3 (70% sweet potato leaves + 30% sweet potato tuber) with three replicates. Rumen fluid samples were taken with stomach tube method using a vacuum pump. Results showed that the T3 diet has higher (P<0.05) digestibility of dry matter and organic matter, concentration of NH3 and VFA, and rumen bacterial population than those of T0 and T1diets but similar to that of T2 diet. Rumen pH and protozoa population was not different among the treatments. It can be concluded that the use of sweet potato biomass can improve the quality of rumen fermentation of sheep.

Energy Metabolism of Sheep Supplemented with Complete Rumen Modifier (CRM)

“…The Complete Rumen Modifier (CRM) in this study includes multiple sources of flavonoids, namely Morinda citrifolia leaf, Ipomoea batatas leaf, and Camelia sinensis leaf dregs (Irawan et al, 2020;Kurniawan, 2018;Sudarman et al, 2016;Tugiyanti et al, 2017) and growth promotor of rumen bacteria such as like Saccharomyces cereviceae, methionine amino acids, and sulfur minerals, (Puastuti, 2009;Wiyatna and Hernaman, 2016). Balcells et al (2012) reported that supplementing 300 mg/kg DM plant extracts containing flavonoids into feed has successfully reduced the risk of acidosis by decreasing the population of lactate-producing bacteria Streptococcus bovis, and increasing the population of lactic-user bacteria Megasphaera elsdenii, pH value, and propionate production.…”