In vitro response of hFOB cells to pamidronate modified sodium silicate coated cellulose scaffolds

Zoledronate, ibandronate and clodronate enhance osteoblast differentiation in a dose dependent manner – A quantitative in vitro gene expression analysis of Dlx5, Runx2, OCN, MSX1 and MSX2

“…They applied higher concentration (3.33 Â 10 À3 M) (Ponader et al, 2008). In agreement with our results, bisphosphonates at lower concentrations did not affect or decrease OCN gene expression (Ponader et al, 2008). In vivo studies as well as clinical trials have demonstrated the bone differentiation potential of zoledronate and ibandronate and therefore support our study results (Black et al, 1996;Liberman et al, 1995;Mortensen et al, 1998).…”

“…Similar, Ponader et al, found a positive differentiation stimulus on osteoblast differentiation by quantitation of alkaline phosphatase (AP) and osteocalcin gene expression after 14 days. They applied higher concentration (3.33 Â 10 À3 M) (Ponader et al, 2008). In agreement with our results, bisphosphonates at lower concentrations did not affect or decrease OCN gene expression (Ponader et al, 2008).…”

“…This bone turnover effect seems to depend on the bisphosphonate concentration. A high dosage leads to impaired mineralization, whereas lower levels do not seem to influence mineralization and osteoblast function, leading to a positive bone balance (Chavassieux et al, 1997;Ponader et al, 2008;Storm et al, 1993). The bisphosphonates are a family of pyrophosphate analogues that can further be separated into nitrogen-containing and non-nitrogen-containing bisphosphonates.…”

The cytotoxic effects of three different bisphosphonates in-vitro on human gingival fibroblasts, osteoblasts and osteogenic sarcoma cells

“…The potency level for ZOL is the highest (>10,000Â), followed by FOS (>100e<1000Â) and thereafter PAM (w100Â) with Etidronate being set as one. Interestingly, Ponader and colleagues describe instead of a toxic effect an enhancement on human osteoblasts of further cell attachments, cellular functions, and cell proliferation and vitality with low concentrations of enriched PAM gels (Ponader et al, 2008). They showed that the lower PAM dosage had the best influence on cell survival and functions.…”

Biologically derived scaffolds

“…Smooth muscle cells (SMCs) attach and proliferate on and into the scaffolds (Backdahl et al, 2008). Further, cellulose hybrid materials such as nanocomposites with hydroxyapatite, gelatin, montmorillonite, cellulose acetate and sodium silicate gel, have been investigated to improve mechanical properties, biocompatibility and cellular adhesion (Fang et al, 2009;Fukuhara et al, 2010;Haroun et al, 2009;Kim et al, 2009;Ponader et al, 2008;Xing et al, 2010).…”