In vitro remineralization of artificial enamel caries with resin composites containing calcium phosphate particles

Abstract

The aim of the study was to evaluate the effect of experimental composites containing dicalcium phosphate dihydrate (DCPD) on remineralization of enamel lesions. Five resin‐based composites containing equal parts (in mols) of bisphenol‐A glycidyl dimethacrylate (BisGMA), triethylene glycol dimethacrylate (TEGDMA), and 60 vol % of fillers were manipulated. Filler phase was constituted by silanized barium glass and 0, 10, or 20 vol % of DPCD particles, either functionalized (F) or nonfunctionalized (NF) with TEGDMA. Artificial subsurface lesions were produced in human enamel fragments and divided according to the resin composite applied on the lesion (no DCPD, 20% NF, 20% F, 10% NF, 10% F) plus a group without composite build‐up (nontreated, NT). Fragments were exposed to 16 days of pH cycling. Specimens were evaluated using transverse microradiography (TMR). Calcium and phosphate concentrations in pH‐cycling solutions were determined by spectrophotometry. TMR and ionic concentrations were analyzed using one‐way ANOVA/Tukey and Kruskal–Wallis/Dunn test, respectively (alpha: 0.05). All composite groups showed enamel remineralization (3%–23%). Higher mineral recovery in the middle (7%–11%) and bottom (2%–7%) thirds of the lesion was observed in groups with DCPD‐containing composites compared to the “no DCPD” group (middle: 1%, bottom: −3%). Lesion depth was significantly reduced in groups using DCPD‐containing composites compared to NT group. No noticeable increase in calcium and phosphate ions was observed in the pH‐cycling solutions due to the presence of DCPD in the composites. In conclusion, composites with DCPD fractions as low as 10%, regardless of functionalization, were able to promote mineral recovery and reduce lesion depth of enamel lesions. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 1542–1550, 2019.

<p>Effect of RSK4 on Biological Characteristics of Gastric Cancer</p>

“…It is the fifth frequently diagnosed cancer and the third leading cause of cancer-related mortality worldwide. 1 In recent years, a large number of investigations have been performed to improve the prognosis of patients with gastric cancer, with neoadjuvant chemotherapy, radiotherapy, and moleculartargeted therapies adopted as efficacious strategies. 19 Despite this, the prognosis of gastric cancer is still dim.…”

“…Gastric cancer is the fifth most common cancer and the third leading cause of cancer-related death in the world, with nearly one million newly diagnosed cases every year. 1 The prognosis of gastric cancer is poor and the average five-year survival rate is less than 20%. 2 Cancer stem cells and metastasis usually cause refractory of gastric cancer.…”

“…3 Furthermore, gastric cancer has caused an estimated 783,000 deaths in 2018. 1 Thus, gastric cancer remains a clinical challenge and finding novel therapeutic targets is imperative.…”

Mechanical properties and surface roughness of polymer-based materials containing DCPD particles

“…Resin composites containing CaP particles remineralize enamel and dentin. 4,7 A significant reduction in dental enamel demineralization has been observed in situ when using composite containing ACP particles. 3 Finally, these materials promote mineral recovery of enamel-dentin affected by caries in vivo.…”

“…However, new development strategies are focusing efforts on regenerative restorative biomaterials 1 capable of reversing the progression of caries lesions due to increasingly prioritized minimal intervention. 2,3,4 The incorporation of particles consisting of different phases of calcium orthophosphates (CaP) in resinous matrices, such as dicalcium phosphate dihydrate (DCPD), amorphous calcium phosphate (ACP), and tetracalcium phosphate (TTCP), are capable of releasing calcium and hydrogen Declaration of Interests: The authors certify that they have no commercial or associative interest that represents a conflict of interest in connection with the manuscript.…”