“…The in vitro secondary caries tests and evaluation methods have been developed that assess demineralized lesions and inhibition zones of dentin due to acid challenge. These methods include polarized light microscopy 7,8,9) , a microhardness test 8,10,11) , microradiography 5,12) , confocal laser-scanning microscopy and the x-ray analytical microscope 13,14) . However, the magnification of such methods is limited if more detailed information at the interface between the cavity and restoration is required.…”