In vitro Regeneration of Plantlets of Curcuma longa Linn. A Valuable Spice Plant in Bangladesh

Rahman, Md. Mahabubur; Amin, Muhammad Nurul; Jahan, Hosne; Ahmed, Rais

doi:10.3923/ajps.2004.306.309

Cited by 30 publications

(13 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Probably this may be attributed due to synergistic effect of BAP and IAA at 2.00 and 0.20mg/l, where in, BAP is known to induce cell division and differentiation and IAA induces cell elongation and cell division. Synergistic effect of auxin with cytokinin in shoot multiplication has been reported by several workers (Jyothibhaskar et al 1993; Rehaman et al 2004) [10,20] . MS B supplemented with BAP 2.00mg/l +IAA 0.20mg/l resulted better shoot initiation (94.44%) of explants.…”

Section: Effect Of Growth Regulators On Shoot Growthmentioning

confidence: 68%

“…The highest numbers of aseptic culture (59.99%) was obtained with HgCl 2 at 0.1 percent for 15 minutes. Bharalee et al (2005) [2] got the results using 0.1 percent HgCl 2 for 15 minutes and Rehman et al (2004) [20] used 0.1 percent HgCl 2 for 14 minutes to establish aseptic cultures in turmeric. These findings are in conformity with the results obtained by Nadgauda et al (1983) in cardamom and De Lange et al…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Standardization of tissue culture protocol for turmeric (Curcuma longa L.) Cv. Salem

Ns¹,

Kn²,

Prabhuling³

et al. 2020

Int. J. Chem. Stud.

View full text Add to dashboard Cite

Turmeric Cv. Salem is the popular cultivar of turmeric growing in Tamil Nadu, Karnataka, Andhra Pradesh and Maharashtra. The demand for planting of this cultivar is huge and tissue culture technology ensures the mass production of a large quantity of disease free true-to-type plants from a single parent plant. The present study was carried during the year 2017-18 to standardize the tissue culture protocol for turmeric to obtain aseptic tissue culture plantlets. Surface sterilization of the explants was effectively achieved by HgCl2 treatment with 0.10% for 15 minutes as it resulted in highest culture establishment (59.99%) with minimum bacterial and fungal contamination. Among the different treatments used for shoot proliferation, MS medium fortified with BAP 2mg/l and IAA 0.2mg/l gave significantly maximum response (94.44%). Maximum in vitro rooting (91.17%) were observed with ½ strength MS medium containing IBA 2mg/l. The rooted plantlets were hardened for 25 days under polytunnel with different media.

show abstract

Section: Effect Of Growth Regulators On Shoot Growthmentioning

confidence: 68%

Section: Resultsmentioning

confidence: 99%

Standardization of tissue culture protocol for turmeric (Curcuma longa L.) Cv. Salem

Ns¹,

Kn²,

Prabhuling³

et al. 2020

Int. J. Chem. Stud.

View full text Add to dashboard Cite

show abstract

“…Sterilization of rhizome buds was done using 0.1% HgCl 2 solution for 10 -20 min to establish aseptic cultures in ginger (Suma et al 2008, Kambaska and Santilata 2009, Khatun et al 2016, turmeric (Rahman et al 2004, Bharalee et al 2005) and black thorn (Rahman et al 2005). Between the two explants, rhizome bud was found to be the most responsive in terms of percentage of shoot regeneration as well as the number of shoots per explant in both the varieties (Table 1).…”

Section: Resultsmentioning

confidence: 99%

In vitro Regeneration of Ginger (Zingiber officinale Roscoe)

Sumon

Banu

Mollika

et al. 2019

Plant Tissue Cult. & Biotech.

View full text Add to dashboard Cite

An efficient and reproducible in vitro regeneration protocol was established for two varieties of ginger (Z. officinale Roscoe) namely, BARI Ada-1 and Chinese ginger accession number SG876). In case of BARI Ada-1 best result was obtained on MS supplemented with 2.0 mg/l BAP, 0.5 mg/l Kn and 0.5 mg/l NAA. In this combination, 95% rhizome bud explants responded within 6 - 8 days and mean number of shoots per explant was 8.79 ± 0.42. On the other hand, Chinese ginger showed best (90%) shoot regeneration response from the same explants on the same medium and hormonal combinations but in exchange of 0.25 mg/l NAA. In this hormonal composition shoot initiation started within 7 - 8 days of culture and mean number of shoots/explant was 6.83 ± 0.71 after 24 - 27 days of culture. Maximum root induction (90 and 80%) was found on MS supplemented with 0.5 mg/l IBA and 0.5 mg/l NAA in case of BARI Ada-1 and Chinese ginger, respectively. The in vitro regenerated plantlets were successfully transplanted into the soil after acclimatization.

show abstract

“…The report is in agreement with our results that 2.5 mg•L À1 BAP and 0.1 mg•L À1 NAA were the best combination for shoot multiplication. Salvi et al (2000) used BA (5 or 10 mg•L À1 ) and NAA (0.1 mg•L À1 ) for direct shoot development, while Rahman et al (2004) (treatment E), but the addition of a low concentration of BAP (treatment F) increased root formation significantly, making it the best treatment for whole plant (with roots) regeneration. Therefore, a low concentration of NAA and BAP (0.1 mg•L À1 ) is needed for the highest root formation.…”

Section: Discussionmentioning

confidence: 99%

Optimizing Turmeric Tissue Culture, Testing Different Media and a Plant Growth Regulator Matrix

Taghavi

Rahemi

Rafie

et al. 2021

hortte

View full text Add to dashboard Cite

Rapid multiplication of turmeric (Curcuma longa) by micropropagation is needed to produce a continuous source of uniformly sized, high-quality, and disease-free plantlets. Three in vitro experiments were conducted to optimize the medium by evaluating nine media and a full factorial combination (matrix) of two plant growth regulators for direct organogenesis of ‘Hawaiian Red’ turmeric. Two experiments evaluated the media, and the third studied the plant growth regulator matrix. As a result, Driver and Kuniyuki walnut (DKW), Murashige and Skoog (MS), and broadleaf tree basal (BLT) media performed better than woody plant media [Lloyd & McCown woody plant basal medium (L&M), and McCown’s woody plant basal salt mixture (McCown)] for shoot and root formation. The multiplication rate was 18 plants per explant in DKW with 1 mg⋅L−1 6-benzylaminopurine (BAP) and 0.1 mg⋅L−1 1-naphthaleneacetic acid (NAA). After transferring the plants to an ex vitro environment, the survival rate was 97%, and 30% higher than previously reported. DKW produced the highest number of plantlets (with shoots and roots), and BLT produced fewer plants with higher biomass. In the MS media, higher BAP to NAA ratio (2.5 to 0.1 mg⋅L−1) produced the most significant number of shoots; however, the lowest concentration of BAP and NAA (0.1 mg⋅L−1 of both) produced the highest number of rooted plantlets. There are two recommendations for tissue culture of ‘Hawaiian Red’ turmeric. To produce the highest number of plantlets, one should use the higher BAP to NAA ratio (2.5 mg⋅L−1 BAP and 0.1 mg⋅L−1 NAA) for shoot proliferation and then transfer the explants to the root initiation media. However, to reduce the number of subcultures, the explants can be grown in the lowest concentration of both BAP and NAA (0.1 mg⋅L−1) to induce both shoot and root. Although, the number of plantlets (with roots and shoots) will decrease in this method, there is no need for subsequent subcultures and changing of the plant growth regulator combinations.

show abstract

In vitro Regeneration of Plantlets of Curcuma longa Linn. A Valuable Spice Plant in Bangladesh

Cited by 30 publications

References 6 publications

Standardization of tissue culture protocol for turmeric (Curcuma longa L.) Cv. Salem

Standardization of tissue culture protocol for turmeric (Curcuma longa L.) Cv. Salem

In vitro Regeneration of Ginger (Zingiber officinale Roscoe)

Optimizing Turmeric Tissue Culture, Testing Different Media and a Plant Growth Regulator Matrix

Contact Info

Product

Resources

About